POLYVALENT VACCINES TO PROTECT POULTRY FROM AVIAN INFLUENZA

• Sponsoring Institution: Agricultural Research Service/USDA
• Project Status: ACTIVE
• Funding Source: USDA INHOUSE
• Reporting Frequency: Annual
• Accession No.: 0426206
• Project Start Date: Jan 1, 2014
• Project End Date: Sep 30, 2016
• Program Code: [(N/A)]- (N/A)

Recipient Organization
AGRICULTURAL RESEARCH SERVICE
(N/A)
ATHENS,GA 30613

Performing Department
(N/A)

Non Technical Summary
(N/A)

Animal Health Component

20%

Research Effort Categories

Basic

50%

Applied

20%

Developmental

30%

Classification

Knowledge Area (KA)	Subject of Investigation (SOI)	Field of Science (FOS)	Percent
311	3210	1090	50%
311	3220	1090	50%

Knowledge Area
311 - Animal Diseases;

Subject Of Investigation
3210 - Egg-type chicken, live animal; 3220 - Meat-type chicken, live animal;

Field Of Science
1090 - Immunology;

Keywords

avian

influenza

vaccine

poultry

virus-like

particle

Goals / Objectives
To evaluate the immunogenicity and efficacy of triple-hemagglutinin (HA) virus-like particles (VLPs) in poultry species as a broadly protective Avian Influenza (AI) vaccine.

Project Methods
Triple-hemagglutinin (HA) virus-like particles (VLPs) will be prepared in Sf9 insect cells using baculovirus expression system, including multi-subtype H5/H7/H9 VLPs, and multi-clade H5N1 vaccine. Characteristics of multi-HA VLPs will be determined including expression levels in Sf9 cell culture, genetic stability and the ratio of HA incorporation into VLPs. Immunogenicity and efficacy of multi-HA VLP vaccines will be evaluated in chicken and turkey species. Protection against homologous and heterologous challenges will be tested including multiple strains of HPAI H5N1 virus.