DEVELOPING USER-FRIENDLY MOLECULAR MARKERS FOR PRIMOCANE FRUITING IN BLACKBERRY

• Sponsoring Institution: Agricultural Research Service/USDA
• Project Status: ACTIVE
• Funding Source: USDA INHOUSE
• Reporting Frequency: Annual
• Accession No.: 0425461
• Project Start Date: Jun 1, 2014
• Project End Date: Jun 20, 2017
• Program Code: [(N/A)]- (N/A)

Recipient Organization
Driscoll Strawberry Associates, Inc.
404 San Juan Road
Watsonville,CA 95076-5399

Performing Department
(N/A)

Non Technical Summary
(N/A)

Animal Health Component

50%

Research Effort Categories

Basic

50%

Applied

50%

Developmental

0%

Classification

Knowledge Area (KA)	Subject of Investigation (SOI)	Field of Science (FOS)	Percent
201	1129	1080	100%

Knowledge Area
201 - Plant Genome, Genetics, and Genetic Mechanisms;

Subject Of Investigation
1129 - Berries and cane fruits, general/other;

Field Of Science
1080 - Genetics;

Keywords

marker-assisted

breeding

marker

development

primocane

fruiting

blackberry

Goals / Objectives
To develop and test blackberry molecular markers to discover new markers that are user-friendly, to permit identification of primocane-fruiting seedlings without having to use expensive equipment.

Project Methods
ARS published blackberry sequences near a genomic region controlling primocane fruiting. The Cooperator will compare those sequences to their proprietary raspberry genome sequences to identify other potential raspberry sequences that could be used to develop additional new blackberry markers near primocane fruiting. ARS will test those markers on the parents and a few selected progeny used to find the published markers. The first testing will be done using the ABI Genetic Analyzer. Markers found to segregate with primocane fruiting will be tested using gel electrophoresis to identify those that are user-friendly,(meaning that they can identify primocane-fruiting progeny by using gel electrophoresis) and can be identifided without the expensive ABI Genetic Analyzer.