IMPROVED DIAGNOSIS OF BOVINE AND EQUINE BABESIOSIS IN EGYPT

• Sponsoring Institution: Agricultural Research Service/USDA
• Project Status: COMPLETE
• Funding Source: USDA INHOUSE
• Reporting Frequency: Annual
• Accession No.: 0422912
• Project Start Date: Nov 3, 2011
• Project End Date: Sep 30, 2012
• Program Code: [(N/A)]- (N/A)

Recipient Organization
AGRICULTURAL RESEARCH SERVICE
(N/A)
PULLMAN,WA 99164

Performing Department
(N/A)

Non Technical Summary
(N/A)

Animal Health Component

30%

Research Effort Categories

Basic

40%

Applied

30%

Developmental

30%

Classification

Knowledge Area (KA)	Subject of Investigation (SOI)	Field of Science (FOS)	Percent
311	3120	1090	20%
311	3310	1110	60%
311	3810	1090	20%

Knowledge Area
311 - Animal Diseases;

Subject Of Investigation
3120 - Spiders, mites, ticks, and other arthropods; 3310 - Beef cattle, live animal; 3810 - Horses, ponies, and mules;

Field Of Science
1090 - Immunology; 1110 - Parasitology;

Keywords

piroplasmosis

babesia

serodiagnosis

horses

bovine

Goals / Objectives
Improve methods for serological diagnosis of bovine and equine babesiosis.

Project Methods
1. An Egyptian researcher will be trained in the techniques to be used for manipulating the samples in the U.S. and in Egypt; 2. Field blood samples from bovines and equines in distinct areas of Egypt will be obtained; 3. The field blood samples will be tested for the presence of Babesia DNA using PCR techniques. 4. The field serum samples will be tested for presence of anti-Babesia antibodies using a competive commercial ELISA test; 5. The data will be analyzed using statistical tests; 6. A manuscript will be prepared for publication in a peer reviewed Journal.

Progress 11/03/11 to 09/30/12

Outputs
Progress Report Objectives (from AD-416): Improve methods for serological diagnosis of bovine and equine babesiosis. Approach (from AD-416): 1. An Egyptian researcher will be trained in the techniques to be used for manipulating the samples in the U.S. and in Egypt; 2. Field blood samples from bovines and equines in distinct areas of Egypt will be obtained; 3. The field blood samples will be tested for the presence of Babesia DNA using PCR techniques. 4. The field serum samples will be tested for presence of anti-Babesia antibodies using a competive commercial ELISA test; 5. The data will be analyzed using statistical tests; 6. A manuscript will be prepared for publication in a peer reviewed Journal. This work relates to objective 1 of parent project by provision of data supporting improved control methods for babesia bovis. An Egyptian Scientist from the Department of Parasitology and Animal Diseases, Veterinary Division, National Research Center, Dokki, Giza, Egypt visited the ARS-USDA labs for a training period in the context of the US-Egypt USAID Grant entitled �Improved diagnosis of bovine and equine Babesiosis in Egypt�. (Project No: 5348-32000-034-04R; Accession No: 0422912. Novel bovine and horse Babesia diagnostic methodologies were transferred to the Egyptian counterpart during this period, including nested PCR, cELISA and immunofluorescence methods.

Impacts
(N/A)

Publications