Source: AGRICULTURAL RESEARCH SERVICE submitted to
INCIDENCE & ANTIMICROBIAL SUSCEPTIBILITY OF FOODBORNE (SALMONELLA & CAMPYLOBACTER) & COMMENSAL BACTERIA FROM THE NAHMS FEEDLOT CATTLE STUDY
Sponsoring Institution
Agricultural Research Service/USDA
Project Status
TERMINATED
Funding Source
USDA INHOUSE
Reporting Frequency
Annual
Accession No.
0422166
Grant No.
(N/A)
Project No.
6612-32000-005-04R
Proposal No.
(N/A)
Multistate No.
(N/A)
Program Code
(N/A)
Project Start Date
Sep 9, 2011
Project End Date
Sep 30, 2013
Grant Year
(N/A)
Project Director
CRAY P J
Recipient Organization
AGRICULTURAL RESEARCH SERVICE
(N/A)
ATHENS,GA 30613
Performing Department
(N/A)
Non Technical Summary
(N/A)
Animal Health Component
(N/A)
Research Effort Categories
Basic
70%
Applied
20%
Developmental
10%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
71233201100100%
Knowledge Area
712 - Protect Food from Contamination by Pathogenic Microorganisms, Parasites, and Naturally Occurring Toxins;

Subject Of Investigation
3320 - Meat, beef cattle;

Field Of Science
1100 - Bacteriology;
Goals / Objectives
Determine the incidence and antimicrobial resistance of Salmonella, Campylobacter, E. coli, enterococcus, and C. difficile from feedlot cattle in the United States.
Project Methods
Fecal samples will be sampled by cultural microbiological techniques to determine bacterial presence. Sensititre microdilution plates will be used to determine antimicroibial resistance.

Progress 09/09/11 to 09/30/13

Outputs
Progress Report Objectives (from AD-416): Determine the incidence and antimicrobial resistance of Salmonella, Campylobacter, E. coli, enterococcus, and C. difficile from feedlot cattle in the United States. Approach (from AD-416): Fecal samples will be sampled by cultural microbiological techniques to determine bacterial presence. Sensititre microdilution plates will be used to determine antimicroibial resistance. This project is related to a portion of objective 1 of this in-house project: To elucidate and provide descriptive data, such as prevalence and/or trends, including antimicrobial susceptibilities, and molecular subtyping for food borne pathogens in food animals through the animal sampling arm of the National Antimicrobial Resistance Monitoring System - Enteric Bacteria (NARMS) program. It is also related to objective 2 of this in-house project: Be a national resource of enteric bacterial isolates and resistance data for food animals from NARMS and USDA-VetNet. We participated in the USDA-APHIS National Animal Health Monitoring System Beef Cattle Feedlot study. Approximately 6000 fecal samples were collected from a nationally representative sampling of beef cattle in feedlots throughout the United States and shipped to the laboratory for isolation of Salmonella, Campylobacter, E. coli, enterococci and Clostridium difficile. Isolates will be characterized for their susceptibility to a panel of antimicrobials.

Impacts
(N/A)

Publications