Source: Henan Academy of Agricultural Sciences submitted to NRP
DETECTION OF MYCOTOXINS WITH FLUORESCENCE POLARIZATION IMMUNOASSAY AND AMBIENT IONIZATION MASS SPECTROMETRY
Sponsoring Institution
Agricultural Research Service/USDA
Project Status
ACTIVE
Funding Source
Reporting Frequency
Annual
Accession No.
0421854
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
Jul 1, 2011
Project End Date
Jun 30, 2016
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
Henan Academy of Agricultural Sciences
No. 116 Hua Yaun Road
Zheng Zhou, Henan,null 450002
Performing Department
(N/A)
Non Technical Summary
(N/A)
Animal Health Component
50%
Research Effort Categories
Basic
30%
Applied
50%
Developmental
20%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
7121510110270%
7121549110230%
Goals / Objectives
Specific objectives are: (1) Transfer a published fluorescence polarization immunoassay (FPIA) method for detecting the fumonisin mycotoxins to Henan Academy of Agricultural Sciences, Agricultural Quality Standards and Testing Technology (HAAS-AQSTT) and introduce HAAS scientists to scientists at American companies interested in FPIA; (2) Transfer an HPLC-MS/MS method for detecting fumonisins to HAAS-AQSTT; and (3) Jointly develop new materials for use in mycotoxin detection.
Project Methods
HAAS-AQSTT and Agricultural Research Service, National Center for Agricultural Utilization Research (NCAUR), Bacterial Foodborne Pathogens & Mycology Research Unit (BFP) will exchange faculty members for research, lectures, and discussions. Specifically, HAAS-AQSTT will send to NCAUR, researchers who will learn the FPIA and HPLC-MS/MS methods in the laboratories of ARS scientists. Visits are anticipated to be of a length from 1 to 3 months and would include at least two scientists from AQSTT. The first scientist would learn the two methods (FPIA and HPLC-MS/MS, objectives 1 & 2). This project would involve developing improved reagents for use in FPIA for fumonisins. Specifically this would involve the synthesis and evaluation of novel fluorescent tracers of fumonisins that might be applied in FPIA. AQSTT will bear the expenses incurred in sending scientists to NCAUR, and their living expenses during the project. NCAUR-BFP would bear the laboratory related expenses (materials, reagents, etc.). It is hoped that this first, initial project will provide a mechanism for future joint research projects involving the development of novel mycotoxin antibodies and the development of ambient ionization mass spectrometry.