DEVELOPMENT OF RAPID DETECTION METHODS USING NANOBIOSENSORS FOR SALMONELLA AND VACCINE AGAINST SE AND ST FOR POULTRY

• Sponsoring Institution: Agricultural Research Service/USDA
• Project Status: ACTIVE
• Funding Source: USDA INHOUSE
• Reporting Frequency: Annual
• Accession No.: 0420863
• Project Start Date: Oct 1, 2010
• Project End Date: Sep 30, 2015
• Program Code: [(N/A)]- (N/A)

Recipient Organization
AGRICULTURAL RESEARCH SERVICE
(N/A)
ATHENS,GA 30613

Performing Department
(N/A)

Non Technical Summary
(N/A)

Animal Health Component

33%

Research Effort Categories

Basic

33%

Applied

33%

Developmental

34%

Classification

Knowledge Area (KA)	Subject of Investigation (SOI)	Field of Science (FOS)	Percent
711	3270	2020	10%
712	3260	2020	90%

Knowledge Area
711 - Ensure Food Products Free of Harmful Chemicals, Including Residues from Agricultural and Other Sources; 712 - Protect Food from Contamination by Pathogenic Microorganisms, Parasites, and Naturally Occurring Toxins;

Subject Of Investigation
3260 - Poultry meat; 3270 - Eggs;

Field Of Science
2020 - Engineering;

Keywords

nanotechnology

biosensor

surface-enhanced

raman

scattering

(sers)

bacteria

pathogen

food

safety

biosecurity

Goals / Objectives
To develop a nanobiosensor to detect Salmonella using surface-enhanced Raman scattering (SERS) technique: (1) determine SERS characteristics of Salmonella; (2) develop chemometric models to differentiate Salmonella from other bacteria; (3) classify different Salmonella strains; and (4) evaluate detection limits of nanobiosensor.

Project Methods
We will develop methodologies to test and validate the SERS techniques. A series of experiments will be conducted based on the following methods: (1) design and fabricate SERS substrate to optimize for lower detection limits; (2) Salmonella sample preparation; (3) SERS measurement; (4) test and validate classification models for different serotypes; and (5) test detectability from different background flora.

Progress 10/01/12 to 09/30/13

Outputs
Progress Report Objectives (from AD-416): To develop a nanobiosensor to detect Salmonella using surface-enhanced Raman scattering (SERS) technique: 1) determine SERS characteristics of Salmonella; 2) develop chemometric models to differentiate Salmonella from other bacteria; 3) classify different Salmonella strains; and 4) evaluate detection limits of nanobiosensor. Approach (from AD-416): We will develop methodologies to test and validate the SERS techniques. A series of experiments will be conducted based on the following methods: 1) Design, fabricate SERS substrate to optimize for lower detection limits; 2) Salmonella sample preparation; 3) SERS measurement; 4) Test and validate classification models for different serotypes; 4) Test detectability from different background flora. This research relates to inhouse objective 1. Develop methods and instruments to identify food safety hazards throughout various stages of poultry and egg production and processing. The protocol for aptamer-based nanobiosensor for specific detection of Salmonella Typhimurium (ST) from food matrix was developed using surface enhanced Raman scattering (SERS) with modified silver nanorod array (AgNR) substrates. Aptamers were immobilized on AgNR substrates followed by immobilization of spacer molecules on AgNR. SERS spectral signatures were obtained from ST cells bound to aptamer-modified SERS substrate. This method improved specificity of nanobiosensor for foodborne pathogen detection.

Impacts
(N/A)

Publications

Progress 10/01/11 to 09/30/12

Outputs
Progress Report Objectives (from AD-416): To develop a nanobiosensor to detect Salmonella using surface-enhanced Raman scattering (SERS) technique: 1) determine SERS characteristics of Salmonella; 2) develop chemometric models to differentiate Salmonella from other bacteria; 3) classify different Salmonella strains; and 4) evaluate detection limits of nanobiosensor. Approach (from AD-416): We will develop methodologies to test and validate the SERS techniques. A series of experiments will be conducted based on the following methods: 1) Design, fabricate SERS substrate to optimize for lower detection limits; 2) Salmonella sample preparation; 3) SERS measurement; 4) Test and validate classification models for different serotypes; 4) Test detectability from different background flora. This research relates to inhouse project objective 1. Develop methods and instruments to identify food safety hazards throughout various stages of poultry and egg production and processing. The protocol for biopolymer encapsulated by silver nanoparticles was developed as surface enhanced Raman scattering (SERS) substrate. The characteristics of silver nano colloidal particle were investigated with morphological analysis, ultraviolet/visible plasma absorption analysis, and plasmonic resonance analysis to examine reproducibility and uniformity of the substrate. The plasmonic absorption peak between 400 and 480 nm was observed from the substrate. SERS with biopolymer silver nano-colloid substrate has potential to detect and classify Salmonella serotypes Typhimurium and Enteritidis.

Impacts
(N/A)

Publications