Progress 09/16/10 to 03/31/13
Outputs
Progress Report Objectives (from AD-416): To determine the population genetic structure of Pythium irregulare, P. sylvaticum, and P. ultimum. Approach (from AD-416): Survey three forest tree nurseries for P. irregulare, P. sylvaticum, and P. ultimum; and investigate the population genetic structure of the three Pythium species by SSR and ISSR analyses for similarities. This research was conducted in support of NP303 objective 2 "Identify plant germplasm and cultivars of small fruits resistant to economically- important soilborne diseases" of the parent project. Pythium species cause conifer seedlings in forest nurseries to become sick and die. We are trying to determine whether Pythium species have been accidentally transferred from nursery to nursery. This is being evaluated by examining the genetics of Pythium species isolated at three forest nurseries. If the genetics of the Pythium isolates are similar at each of the three nurseries, this will provide evidence that movement from nursery to nursery has occurred. However, if the genetics of the Pythium isolates are different at each of the three nurseries, this will provide evidence that movement from nursery to nursery has not occurred. Analyses on three Pythium species (P. irregulare, P. sylvaticum, and P. ultimum) have been nearly completed and suggest that there has been frequent movement of Pythium species between the three nurseries. This information is important to develop disease control measures that minimize the movement of important nursery pathogens from nursery to nursery.
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Progress 10/01/11 to 09/30/12
Outputs
Progress Report Objectives (from AD-416): To determine the population genetic structure of Pythium irregulare, P. sylvaticum, and P. ultimum. Approach (from AD-416): Survey three forest tree nurseries for P. irregulare, P. sylvaticum, and P. ultimum; and investigate the population genetic structure of the three Pythium species by SSR and ISSR analyses for similarities. Completed population analyses of Pythium irregulare and Pythium sylvaticum from three forest nurseries. Analyses of Pythium ultimum are in progress and near completion. There was no evidence that Pythium isolates from one nursery were significantly genetically different from those at the other nurseries, which may indicate that there has been movement of the three pathogens among nurseries. Information regarding the genetic diversity and potential movement of Pythium species among nurseries is useful for the management of Pythium diseases. This research was conducted in support of objective 303 2C Ecology and Epidemiology of Plant Diseases of the parent project.
Impacts
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