Progress 03/26/10 to 09/30/10
Outputs
Progress Report Objectives (from AD-416) To accelerate and enhance the formulation of high performance insect diets that improve the fitness of mass reared insects used in research and insects used in the biological control of insect and weed pests by: 1) improving the formulation of artificial diets and diet-delivery systems; 2) determining the impact of nutrient substitutions on the efficiency of diet utilization; and 3) developing genomic biomarkers to monitor fitness traits related to nutrition. Approach (from AD-416) Formulation changes will be made to micronutrient levels and substitutions will be made for the fibrous, temperature sensitive and antibiotic materials in artificial diets as part of an empirical-based effort to improve artificial diets. Life history measurements will be used to assess the impact those dietary changes on the health of the insect. The impact of dietary component changes on the biochemical fate of nutrients will be monitored. Enzymatic activity and metabolism of nutrients will be used to assess the impact of dietary changes on the efficiency of nutrient absorbance and nutrient utilization. The use of genomic biomarkers as indicators of fitness for insects reared on diets varying in nutritional quality will be investigated. Differentially- expressed genes will be identified using suppressive subtractive hybridization and microarray analyses. This project ended on 9/30/10 and was replaced with 3622-22000-035-00D. We fully met and exceeded the intent of objective 1 which focused on the biology of natural enemies. The research treated beneficial and pest insects, including a beneficial predatory insect species, a pest fruit fly species used in sterile-insect-technology programs and a new invasive insect that vectors a disease of grapes. We substantially met the intent of objective 2, which was to determine the impact of nutrient substitutions on the efficiency of diet utilization. Knowledge of the biochemical fate of several dietary components has been significantly increased and that knowledge is being used by several research groups. We fully met and exceeded the intent of objective 3, which was to develop genomic biomarkers to monitor fitness traits related to nutrition. We documented the potential use of the technology, indentified genes that may be developed into useful biomarkers and transferred the methods to other researchers.
Impacts
(N/A)
Publications
- Alhaddad, H., Coudron, T.A., Backus, E.A., Schreiber, F. 2011. Comparative behavioral and protein study of salivary secretions in Homalodisca spp. sharpshooters (Hemiptera: Cicadellidae: Cicadellinae). Annals of the Entomological Society of America. 104:543-552.
- Coudron, T.A., Shelby, K., Ellersieck, M.R., Winston, B.R., Popham, H.J. 2011. Developmental response of the beneficial predator Podisus maculiventris to change in dietary ascorbic acid concentration. Entomologia Experimentalis et Applicata. 139:235-241.
- Buyukguzel, E., Tunaz, H., Stanley, D.W., Buyukguzel, K. 2011. Influence of chronic eicosanoid biosynthesis inhibition on life history of the greater waxmoth, Galaleria mellonella and its ectoparasitoid, Bracon hebetor. Journal of Insect Physiology. 57:501-507.
- Lim, E., Roh, H., Coudron, T.A., Park, G. 2011. Temperature-dependent fumigant toxicity of some essential oils and their main constituents against two-spotted spider mite, Tetranychus urticae. Journal of Economic Entomology. 104(2):414-419.
- Tian, L., Ji, B., Liu, S., He, C., Jin, F., Gao, J., Stanley, D.W., Li, S. 2010. JH biosynthesis by reproductive tissues and corpora allata in adult longhorned beetles, Apriona germari. Archives of Insect Biochemistry and Physiology. 75(4):275-286.
- Ladurantaye, Y., Khelifi, M., Cloutier, C., Coudron, T.A. 2010. Short term storage conditions for transport and farm delivery of the stink bug Perillus bioculatus for the biological control of the Colorado potato beetle. Biological Engineering (ASABE). 52:1-7.
- Coudron, T.A., Chang, C.L., Goodman, C.L., Stanley, D.W. 2011. Dietary wheat germ oil influences gene expression in larvae and eggs of the oriental fruit fly. Archives of Insect Biochemistry and Physiology. 76:67- 82.
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