Source: MONTCROFT FARMS submitted to NRP
POTATO FARMING
Sponsoring Institution
Agricultural Research Service/USDA
Project Status
COMPLETE
Funding Source
USDA CONTRACT
Reporting Frequency
Annual
Accession No.
0418153
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
Aug 1, 2009
Project End Date
Oct 31, 2009
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
MONTCROFT FARMS
797 POTATO FARM ROAD
TERRA ALTA,WV 26764-7205
Performing Department
(N/A)
Non Technical Summary
(N/A)
Animal Health Component
10%
Research Effort Categories
Basic
90%
Applied
10%
Developmental
0%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
21213101160100%
Knowledge Area
212 - Pathogens and Nematodes Affecting Plants;

Subject Of Investigation
1310 - Potato;

Field Of Science
1160 - Pathology;
Keywords
Goals / Objectives
Document the current population structure of P. infestans in North America using genotypic and phenotypic data; Isolates of P. infestans will be collected from this major potato growing regions in the Northeast. We will collect phenotypic data such as mating type, metalaxyl sensitivity, aggressiveness, virulence, and genotypic data including mitochondrial haplotypes, AFLP genotype, allozyme genotype, and SSR genotypes. A standard set of SSR markers will be critical to the success of the project. A single multiplexed set of 8-10 SSR markers will be standardized at the Scottish Crops Research Institute (SCRI, David Cooke) and made available for this project that represent the best compromise in terms of allelic diversity, ease of scoring and suitability for the North American populations.
Project Methods
This 1.5 acre trap plot of various cultivars of tomato and potatoes with known genetic backgrounds will be planted and cultivated without chemical protective fungicide sprays. Data will be taken on growth and health of plants. Samples of disease tissues will be collected and sent to the GIFVL lab for processing and disease analysis.

Progress 08/01/09 to 10/31/09

Outputs
Progress Report Objectives (from AD-416) Document the current population structure of P. infestans in North America using genotypic and phenotypic data; Isolates of P. infestans will be collected from this major potato growing regions in the Northeast. We will collect phenotypic data such as mating type, metalaxyl sensitivity, aggressiveness, virulence, and genotypic data including mitochondrial haplotypes, AFLP genotype, allozyme genotype, and SSR genotypes. A standard set of SSR markers will be critical to the success of the project. A single multiplexed set of 8-10 SSR markers will be standardized at the Scottish Crops Research Institute (SCRI, David Cooke) and made available for this project that represent the best compromise in terms of allelic diversity, ease of scoring and suitability for the North American populations. Approach (from AD-416) This 1.5 acre trap plot of various cultivars of tomato and potatoes with known genetic backgrounds will be planted and cultivated without chemical protective fungicide sprays. Data will be taken on growth and health of plants. Samples of disease tissues will be collected and sent to the GIFVL lab for processing and disease analysis. This 1.5 acre sentinel plot of various cultivars of tomato and potatoes with known genetic backgrounds were planted and cultivated without chemical protective fungicide sprays. Data was taken on growth and health of plants. Samples of disease tissues was collected and sent to the GIFVL lab for processing and disease analysis. Progress was monitored by telephone calls and site visits.

Impacts
(N/A)

Publications