Progress 10/01/11 to 09/30/12
Outputs
Progress Report Objectives (from AD-416): The main objective is to significantly speed the analytical detection process for hundreds of pesticide and veterinary drug residues in different types of foods. The proposed research is designed to further develop supersonic molecular beam mass spectrometry (SMB-MS) technology with its related sample preparation and operation methods to provide ultra-fast analysis of pesticides, drugs, and environmental contaminants in foods with full analysis cycle time of < 1 min. We shall explore: a) unique high flow rate universal SMB-MS for gas chromatography; b) novel open probe SMB- MS/MS as an even faster alternative; c) liquid flow injection SMB-MS/MS to eliminate ion suppression effects; and d) fast SMB-MS for liquid chromatography. Appropriate sampling protocols and sample preparation methods will be devised for the ultra-fast detection approaches to ensure meaningful results. Emphasis will be given to address the needs of fast pesticide and veterinary drug analysis prior to food distribution and to meet the ultra-fast analytical demands of possible food terrorism scenarios. Approach (from AD-416): We shall explore new and novel sample preparation approaches for the multiclass, multiresidue analysis of drug residues in foods. Prof. Amirav will further develop supersonic molecular beam mass spectrometry, particularly using liquid chromatography for the polar drug analytes rather than gas chromatography as was the case for pesticides. The main objective of this project is to significantly speed the analytical detection process for hundreds of pesticide and veterinary drug residues in different types of foods. The research is predominantly designed to further develop supersonic molecular beam mass spectrometry (SMB-MS) technology with its related sample preparation and operation methods to provide ultra-fast analysis of pesticides, drugs, and environmental contaminants in foods. Prof. Aviv Amirav, the collaborator in Israel, has made excellent progress on the ultra-fast SMB-MS analysis component of the project with the goal of less than 1 min analysis time. During the third year in the ARS lab, we extended the �Goldilocks� method to twice as many veterinary drug residues in food animal tissues as in the previous year. More than 120 veterinary drugs have now been included in the method, which was developed, validated, and transferred to the USDA Food Safety Inspection Service. Further studies designed to optimize the extraction, cleanup, and detection of the veterinary drug residues in animal tissues were conducted, including a rigorous assessment of matrix effects on qualitative and quantitative analysis. A single analyst can process 60 samples per 8 hour day using this method followed by sequential analyses of 10 min each using ultra-performance liquid chromatography � triple quadrupole tandem mass spectrometry. This analytical method is capable of simultaneous identification and quantification of >100 veterinary drugs at regulatory tolerance levels in beef kidney, muscle, and other tissue types. The project is on schedule for completion at the end of the fiscal year.
Impacts
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Publications
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Progress 10/01/10 to 09/30/11
Outputs
Progress Report Objectives (from AD-416) The main objective is to significantly speed the analytical detection process for hundreds of pesticide and veterinary drug residues in different types of foods. The proposed research is designed to further develop supersonic molecular beam mass spectrometry (SMB-MS) technology with its related sample preparation and operation methods to provide ultra-fast analysis of pesticides, drugs, and environmental contaminants in foods with full analysis cycle time of < 1 min. We shall explore: a) unique high flow rate universal SMB-MS for gas chromatography; b) novel open probe SMB- MS/MS as an even faster alternative; c) liquid flow injection SMB-MS/MS to eliminate ion suppression effects; and d) fast SMB-MS for liquid chromatography. Appropriate sampling protocols and sample preparation methods will be devised for the ultra-fast detection approaches to ensure meaningful results. Emphasis will be given to address the needs of fast pesticide and veterinary drug analysis prior to food distribution and to meet the ultra-fast analytical demands of possible food terrorism scenarios. Approach (from AD-416) We shall explore new and novel sample preparation approaches for the multiclass, multiresidue analysis of drug residues in foods. Prof. Amirav will further develop supersonic molecular beam mass spectrometry, particularly using liquid chromatography for the polar drug analytes rather than gas chromatography as was the case for pesticides. The main objective of this project is to significantly speed the analytical detection process for hundreds of pesticide and veterinary drug residues in different types of foods. The research is predominantly designed to further develop supersonic molecular beam mass spectrometry (SMB-MS) technology with its related sample preparation and operation methods to provide ultra-fast analysis of pesticides, drugs, and environmental contaminants in foods. Prof. Aviv Amirav, the collaborator in Israel, has made excellent progress on the ultra-fast SMB-MS analysis component of the project with the goal of <1 min analysis time. During the second year in the ARS lab, we developed, validated, and transferred to the USDA Food Safety Inspection Service a rapid sample preparation and analysis procedure that we dub �Goldilocks� because it is designed to be �just right� for the extraction, cleanup, and detection of >60 veterinary drug residues in animal tissues. A single analyst can process 60 samples per 8 hour day using this method followed by sequential analyses of 10 min each using ultra-performance liquid chromatography � triple quadrupole tandem mass spectrometry. This analytical method is capable of simultaneous identification and quantification of >60 veterinary drugs at regulatory tolerance levels in beef kidney, muscle, and other tissue types. The project is progressing well and is on schedule.
Impacts
(N/A)
Publications
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Progress 10/01/09 to 09/30/10
Outputs
Progress Report Objectives (from AD-416) The main objective is to significantly speed the analytical detection process for hundreds of pesticide and veterinary drug residues in different types of foods. The proposed research is designed to further develop supersonic molecular beam mass spectrometry (SMB-MS) technology with its related sample preparation and operation methods to provide ultra-fast analysis of pesticides, drugs, and environmental contaminants in foods with full analysis cycle time of < 1 min. We shall explore: a) unique high flow rate universal SMB-MS for gas chromatography; b) novel open probe SMB- MS/MS as an even faster alternative; c) liquid flow injection SMB-MS/MS to eliminate ion suppression effects; and d) fast SMB-MS for liquid chromatography. Appropriate sampling protocols and sample preparation methods will be devised for the ultra-fast detection approaches to ensure meaningful results. Emphasis will be given to address the needs of fast pesticide and veterinary drug analysis prior to food distribution and to meet the ultra-fast analytical demands of possible food terrorism scenarios. Approach (from AD-416) We shall explore new and novel sample preparation approaches for the multiclass, multiresidue analysis of drug residues in foods. Prof. Amirav will further develop supersonic molecular beam mass spectrometry, particularly using liquid chromatography for the polar drug analytes rather than gas chromatography as was the case for pesticides. The main objective of this project is to significantly speed the analytical detection process for hundreds of pesticide and veterinary drug residues in different types of foods. The research is predominantly designed to further develop supersonic molecular beam mass spectrometry (SMB-MS) technology with its related sample preparation and operation methods to provide ultra-fast analysis of pesticides, drugs, and environmental contaminants in foods with full analysis cycle time of < 1 min. We are exploring a unique high flow rate universal SMB-MS for gas chromatography (GC), a novel open probe SMB-MS/MS as an even faster alternative, a liquid flow injection SMB-MS/MS to eliminate ion suppression effects, and a fast SMB-MS for liquid chromatography (LC). Sampling protocols and sample preparation methods are being devised for the ultra-fast detection approaches to ensure meaningful results. Emphasis is given to address the needs of fast pesticide and veterinary drug analysis prior to food distribution and to meet the ultra-fast analytical demands of possible food terrorism scenarios. In the first year of the project, we have improved upon the analysis of notoriously difficult-to-analyze fungicides, captan, folpet, and captafol, using GC- SMB/MS. This is critical in the overall analysis scheme to include as many residues as possible. We also devised low-pressure GC-MS/MS conditions for 150 pesticides in a 10 minute analysis. Validation of the method using the harmonized QuEChERS version for widely different food matrices were completed, plus qualitative identification of the randomly added pesticides as low as 10 ng/g in blind samples was conducted. The project is progressing well and is on schedule. Communication with the collaborators was through email and conference calls.
Impacts
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Publications
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