Progress 10/01/10 to 09/30/11
Outputs
Progress Report Objectives (from AD-416) 1. Identify and prioritize valuable sources of citrus germplasm in Florida which threatened by HLB or citrus canker. 2. Establish the selected germplasm at USDA ARS Ft. Pierce and subject to standardized thermotherapy procedures. Following thermotherapy of germplasm test for diseases which should be eliminated, including HLB and citrus canker. 3. Once thermotherapied germplasm has tested free HLB and citrus canker and other pathogens which should be eliminated, budwood would be shipped to NCGRCD, Riverside where it would be subjected to shoot tip grafting, followed by biological and laboratory indexing to ensure elimination of all citrus graft transmissible pathogens as required for release from quarantine in NCGRCD by CDFA and USDA APHIS. 4. Germplasm released from quarantine at NCGRCD would be sent to FL Citrus Introduction Program which will test the repatriated germplasm via protocols established by the �Citrus Passport� protocol, and release to the industry, keeping a clean source in the DPI facility in Chiefland. Approach (from AD-416) A committee composed of members from the citrus breeding teams from University of Florida, USDA ARS, Florida Budwood Program, NCGRCD, and members of the Florida Citrus Production Managers Association, Florida Nurserymans� Association, and Florida Citrus Mutual would identify and prioritize elite germplasm vital for the short and long term survival of the Florida citrus industry and which is threatened by presence of exotic diseases. A technician, under the supervision of E. Stover, would establish the plants and conduct the thermotherapy at ARS, Ft. Pierce, FL. Personnel from ARS, Riverside and Florida CIP would conduct laboratory testing on plants at Ft. Pierce following the growout after thermotherapy. Once freedom from HLB and citrus canker is confirmed, budwood would be shipped under quarantine to the Repository, Riverside and some to Florida CIP, Gainesville, where shoot tip grafting followed by full biological indexing would be performed. Following release from quarantine, pathogen- tested budwood would be returned to Florida with a plant maintained in the Protected Collection at the Repository. The purpose of this project is to preserve citrus germplasm in Florida that is threatened by loss due to huanglongbing (HLB) and citrus canker. This research is in cooperation with the USDA ARS U.S. Horticultural Research Laboratory, Ft. Pierce, FL and the Florida Citrus Germplasm Introduction Program and relates to objective 3 of the parent project. Fifty unique accessions from Florida have been propagated in a secure greenhouse, repropagated with buds treated with antibiotics, and appear to be free of HLB. These accessions are being tested for HLB, further therapies as needed and tested for other citrus pathogens. The Repository, Riverside has received 22 accessions from Florida, and 11 are nearly completion of therapy and indexing for release from quarantine status.
Impacts
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Progress 10/01/09 to 09/30/10
Outputs
Progress Report Objectives (from AD-416) 1. Identify and prioritize valuable sources of citrus germplasm in Florida which threatened by HLB or citrus canker. 2. Establish the selected germplasm at USDA ARS Ft. Pierce and subject to standardized thermotherapy procedures. Following thermotherapy of germplasm test for diseases which should be eliminated, including HLB and citrus canker. 3. Once thermotherapied germplasm has tested free HLB and citrus canker and other pathogens which should be eliminated, budwood would be shipped to NCGRCD, Riverside where it would be subjected to shoot tip grafting, followed by biological and laboratory indexing to ensure elimination of all citrus graft transmissible pathogens as required for release from quarantine in NCGRCD by CDFA and USDA APHIS. 4. Germplasm released from quarantine at NCGRCD would be sent to FL Citrus Introduction Program which will test the repatriated germplasm via protocols established by the �Citrus Passport� protocol, and release to the industry, keeping a clean source in the DPI facility in Chiefland. Approach (from AD-416) A committee composed of members from the citrus breeding teams from University of Florida, USDA ARS, Florida Budwood Program, NCGRCD, and members of the Florida Citrus Production Managers Association, Florida Nurserymans� Association, and Florida Citrus Mutual would identify and prioritize elite germplasm vital for the short and long term survival of the Florida citrus industry and which is threatened by presence of exotic diseases. A technician, under the supervision of E. Stover, would establish the plants and conduct the thermotherapy at ARS, Ft. Pierce, FL. Personnel from ARS, Riverside and Florida CIP would conduct laboratory testing on plants at Ft. Pierce following the growout after thermotherapy. Once freedom from HLB and citrus canker is confirmed, budwood would be shipped under quarantine to the Repository, Riverside and some to Florida CIP, Gainesville, where shoot tip grafting followed by full biological indexing would be performed. Following release from quarantine, pathogen- tested budwood would be returned to Florida with a plant maintained in the Protected Collection at the Repository. Documents Trust with National Academy of Science (FL Citrus Production Research Council). Log 37339. The purpose of this project is to preserve citrus germplasm in Florida that is threatened by loss due to huanglongbing (HLB) and citrus canker. Research is being performed to determine if antibiotic and/or heat treatment of budwood will remove HLB from budwood. This research is in cooperation with the USDA ARS U.S. Horticultural Research Laboratory (USHRL), Ft. Pierce, FL. In California, because HLB is not present, experiments are being performed using citrus stubborn, caused by Spiroplasma citri, and using tomatoes and the bacterium associated with tomato psyllid yellows. The National Clonal Germplasm Repository for Citrus and Dates, Riverside, has received 17 accessions from the USHRL, Ft. Pierce, and four from Division of Plant Industry (DPI), Winter Haven. These accessions are currently going through therapy and indexing.
Impacts
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Progress 10/01/08 to 09/30/09
Outputs
Progress Report Objectives (from AD-416) 1. Identify and prioritize valuable sources of citrus germplasm in Florida which threatened by HLB or citrus canker. 2. Establish the selected germplasm at USDA ARS Ft. Pierce and subject to standardized thermotherapy procedures. Following thermotherapy of germplasm test for diseases which should be eliminated, including HLB and citrus canker. 3. Once thermotherapied germplasm has tested free HLB and citrus canker and other pathogens which should be eliminated, budwood would be shipped to NCGRCD, Riverside where it would be subjected to shoot tip grafting, followed by biological and laboratory indexing to ensure elimination of all citrus graft transmissible pathogens as required for release from quarantine in NCGRCD by CDFA and USDA APHIS. 4. Germplasm released from quarantine at NCGRCD would be sent to FL Citrus Introduction Program which will test the repatriated germplasm via protocols established by the �Citrus Passport� protocol, and release to the industry, keeping a clean source in the DPI facility in Chiefland. Approach (from AD-416) A committee composed of members from the citrus breeding teams from University of Florida, USDA ARS, Florida Budwood Program, NCGRCD, and members of the Florida Citrus Production Managers Association, Florida Nurserymans� Association, and Florida Citrus Mutual would identify and prioritize elite germplasm vital for the short and long term survival of the Florida citrus industry and which is threatened by presence of exotic diseases. A technician, under the supervision of E. Stover, would establish the plants and conduct the thermotherapy at ARS, Ft. Pierce, FL. Personnel from ARS, Riverside and Florida CIP would conduct laboratory testing on plants at Ft. Pierce following the growout after thermotherapy. Once freedom from HLB and citrus canker is confirmed, budwood would be shipped under quarantine to the Repository, Riverside and some to Florida CIP, Gainesville, where shoot tip grafting followed by full biological indexing would be performed. Following release from quarantine, pathogen- tested budwood would be returned to Florida with a plant maintained in the Protected Collection at the Repository. Documents Trust with National Academy of Science (FL Citrus Production Research Council). Log 37339. Significant Activities that Support Special Target Populations The purpose of this project is to preserve citrus germplasm in Florida that is threatened by loss due to huanglongbing (HLB) and citrus canker. A meeting was held at the University of Florida (UF)�s Citrus Research and Education Center, Lake Alfred on June 30, 2009 with the purpose of discussing and identifying what citrus germplasm needs to be rescued from Florida and to prioritize the order of rescue. There were 25 participants at the meeting which included members of the Crop Germplasm Committee for the USDA ARS National Clonal Germplasm Repository for Citrus and Dates, Riverside with additional participants invited to provide better representation of Florida needs and information as to current status of Florida germplasm. The protocol to be used for recovery, therapy, and release from quarantine of the selected germplasm was discussed, as well as the differing needs of the USDA ARS and the University of Florida citrus breeding groups. For this project, the investigators have been developing alterative approaches to therapy by use of heat and antibiotics, and the group encouraged further research in this direction. Criteria were established for inclusion when submitting candidate varieties for recovery. The Repository, Riverside, has received 17 accessions from the USHRL, Ft. Pierce, and four from DPI, Winter Haven. These accessions are currently going through therapy and indexing.
Impacts
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