Source: PIONEER HI-BRED INTERNATIONAL, INC. submitted to NRP
CORN SEED CHIPPING FOR RAPID DNA EXTRACTION
Sponsoring Institution
Agricultural Research Service/USDA
Project Status
ACTIVE
Funding Source
Reporting Frequency
Annual
Accession No.
0416620
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
May 1, 2009
Project End Date
Apr 30, 2014
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
PIONEER HI-BRED INTERNATIONAL, INC.
7300 N.W. 62ND AVENUE, P.O. BOX 1004
JOHNSTON,IA 50131
Performing Department
(N/A)
Non Technical Summary
(N/A)
Animal Health Component
10%
Research Effort Categories
Basic
90%
Applied
10%
Developmental
0%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
20315101080100%
Goals / Objectives
Identify recombinant progeny for fine-mapping of genome regions for identifying flowering time genes.
Project Methods
Transfer kernel samples from 200 ears of ARS corn lines NIL-0028 x E73 F2 to cooperator for kernel chipping utilizing specialized equipment for automated seed chipping. Cooperator will provide resulting chipped kernels and seed chips back to ARS for extracting DNA, genotyping with SNP markers and identifying recombinant progeny.

Progress 10/01/12 to 09/30/13

Outputs
Progress Report Objectives (from AD-416): Identify recombinant progeny for fine-mapping of genome regions for identifying flowering time genes. Approach (from AD-416): Transfer kernel samples from 200 ears of ARS corn lines NIL-0028 x E73 F2 to cooperator for kernel chipping utilizing specialized equipment for automated seed chipping. Cooperator will provide resulting chipped kernels and seed chips back to ARS for extracting DNA, genotyping with SNP markers and identifying recombinant progeny. This report serves to document research conducted under a Specific Cooperative Agreement between ARS and Pioneer Hi-Bred International, Inc. Additional details of research can be found in the report for the parent project 6645-21220-015-00 �Genetic Analysis of Complex Traits in Maize�. This project is related to Objective 1A of the parent project to create new genetic stocks to identify favorable alleles for yield improvement derived from exotic maize germplasm.. The objective of this experiment is to obtain small seed chips from many corn kernels arrayed in a plate suitable for rapid DNA extraction along with the remaining parental seeds arrayed in a separate plate in the same order. We will use the seed chips to extract DNA, genotype the DNA samples with DNA markers, identify seeds with genotypes of interest, and select the appropriate seeds for planting. To date, 220 ears were chipped and seeds were genotyped to permit selection for desired genetic groups for planting.

Impacts
(N/A)

Publications


    Progress 10/01/11 to 09/30/12

    Outputs
    Progress Report Objectives (from AD-416): Identify recombinant progeny for fine-mapping of genome regions for identifying flowering time genes. Approach (from AD-416): Transfer kernel samples from 200 ears of ARS corn lines NIL-0028 x E73 F2 to cooperator for kernel chipping utilizing specialized equipment for automated seed chipping. Cooperator will provide resulting chipped kernels and seed chips back to ARS for extracting DNA, genotyping with SNP markers and identifying recombinant progeny. This project is related to Objective 1 of the parent project to identify genes and genome regions controlling key traits in diverse maize germplasm using multiple populations and novel genetic mapping methods. The objective of this experiment is to obtain small seed chips from many corn kernels arrayed in a plate suitable for rapid DNA extraction along with the remaining parental seeds arrayed in a separate plate in the same order. We will use the seed chips to extract DNA, genotype the DNA samples with DNA markers, identify seeds with genotypes of interest, and select the appropriate seeds for planting. To date, 20 ears were chipped and seeds were genotyped to permit selection for desired genetic groups for planting.

    Impacts
    (N/A)

    Publications


      Progress 10/01/10 to 09/30/11

      Outputs
      Progress Report Objectives (from AD-416) Identify recombinant progeny for fine-mapping of genome regions for identifying flowering time genes. Approach (from AD-416) Transfer kernel samples from 200 ears of ARS corn lines NIL-0028 x E73 F2 to cooperator for kernel chipping utilizing specialized equipment for automated seed chipping. Cooperator will provide resulting chipped kernels and seed chips back to ARS for extracting DNA, genotyping with SNP markers and identifying recombinant progeny. Related to inhouse objective 1A: Identifying genome regions and specific genes controlling the response to photoperiod in multiple tropical maize inbreds. The objective of this experiment is to obtain small seed chips from many corn kernels arrayed in a plate suitable for rapid deoxyribonucleic acid (DNA) extraction along with the remaining parental seeds arrayed in a separate plate in the same order. We will use the seed chips to extract DNA, genotype the DNA samples with DNA markers, identify seeds with genotypes of interest, and select the appropriate seeds for planting. To date, 20 ears were chipped and seeds were genotyped to permit selection for desired genetic groups for planting. The project was monitored by progress updates exchanged by email and telephone calls between Pioneer Hi-Bred scientists and ARS scientists.

      Impacts
      (N/A)

      Publications


        Progress 10/01/09 to 09/30/10

        Outputs
        Progress Report Objectives (from AD-416) Identify recombinant progeny for fine-mapping of genome regions for identifying flowering time genes. Approach (from AD-416) Transfer kernel samples from 200 ears of ARS corn lines NIL-0028 x E73 F2 to cooperator for kernel chipping utilizing specialized equipment for automated seed chipping. Cooperator will provide resulting chipped kernels and seed chips back to ARS for extracting DNA, genotyping with SNP markers and identifying recombinant progeny. This research relates to inhouse objective 1: Identify genes and genome regions controlling key traits in diverse maize germplasm using multiple populations and novel genetic mapping methods. The objective of this experiment is to obtain small seed chips from many corn kernels arrayed in a plate suitable for rapid DNA extraction along with the remaining parental seeds arrayed in a separate plate in the same order. We will use the seed chips to extract DNA, genotype the DNA samples with DNA markers, identify seeds with genotypes of interest, and select the appropriate seeds for planting. To date, 20 ears were chipped and seeds were genotyped to permit selection for desired genetic groups for planting. The project was monitored by progress updates exchanged by email and telephone calls between Pioneer Hi-Bred scientists and ARS scientists.

        Impacts
        (N/A)

        Publications


          Progress 10/01/08 to 09/30/09

          Outputs
          Progress Report Objectives (from AD-416) Identify recombinant progeny for fine-mapping of genome regions for identifying flowering time genes. Approach (from AD-416) Transfer kernel samples from 200 ears of ARS corn lines NIL-0028 x E73 F2 to cooperator for kernel chipping utilizing specialized equipment for automated seed chipping. Cooperator will provide resulting chipped kernels and seed chips back to ARS for extracting DNA, genotyping with SNP markers and identifying recombinant progeny. Significant Activities that Support Special Target Populations This project relates to inhouse objective 1: Identify genes and genome regions controlling key traits in diverse maize germplasm using multiple populations and novel genetic mapping methods. The objective of this experiment is to obtain small seed chips from many corn kernels arrayed in a plate suitable for rapid DNA extraction along with the remaining parental seeds arrayed in a separate plate in the same order. We will use the seed chips to extract DNA, genotype the DNA samples with DNA markers, identify seeds with genotypes of interest, and select the appropriate seeds for planting. To date, no seeds have been sent to Pioneer for chipping. Progress was monitored by updates exchanged by email and telephone calls between Pioneer Hi-Bred scientists and ARS scientists.

          Impacts
          (N/A)

          Publications