Progress 01/01/09 to 12/31/11
Outputs
Progress Report Objectives (from AD-416): The discovery of genes or quantitative trait loci (QTL) that control resistance in soybean to the soybean rust pathogen. Approach (from AD-416): Segregating populations being developed using 81 Plant Introductions (PIs) with putative resistance to soybean rust will be screened by the various collaborators to identify a set of the most resistant and a set of the most susceptible lines from each population in order to apply bulked segregant analysis (BSA). The resistant and susceptible bulks from each population, along with the parents will be screened using the Universal 1536 Soy Linkage Panel 1.1 (USLP 1.1) and analyzed on the Illumina BeadStation 500G. In those instances where resistance appears to be quantitatively inherited (slow rusting), the entire population will be tested with the USLP 1.1 in order to map the quantitative trait loci (QTL) controlling resistance. In some instances fine mapping and haplotyping of resistance loci will also be completed. Funds from the University of Illinois, Urbana, IL are provided by the United Soybean Board, as part of Project USB Project #9235 entitled Identification and Utilization of Resistance to Soybean Rust. The specific goal of the collaborative research is to determine the genome locations of soybean rust resistance genes to allow selection of resistance in the absence of the disease and to facilitate the combining of different rust resistance genes in a single soybean variety. This research uses the 1536 single nucleotide polymorphism (SNP) DNA markers of the Universal Soybean Linkage Panel 1.0 (USLP 1.0) to analyze breeding populations in which genes for resistance to soybean rust are present. This will allow the determination of the genome positions of resistance genes and will identify DNA markers that can be used to select soybean breeding lines that carry the resistance genes. To this end a total of 70 samples from collaborators at the University of Georgia were analyzed with the USLP 1.0 SNPs. The samples included the rust susceptible adapted parental genotypes Boggs, G00-3880, LD00-3309, R00-1194F and Williams 82. A total of 17 Plant Introductions with resistance to soybean rust that had been crossed with one of the susceptible parents were among the 70 genotypes. In addition, 48 bulk populations derived from 17 different crosses of the adapted parents with the rust resistant parents were analyzed with the 1536 SNPs. Both rust susceptible and rust resistance bulks were included among the 48 DNA bulk samples. The resulting genotypic data were forwarded to the University of Georgia for further analysis.
Impacts
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Progress 10/01/10 to 09/30/11
Outputs
Progress Report Objectives (from AD-416) The discovery of genes or quantitative trait loci (QTL) that control resistance in soybean to the soybean rust pathogen. Approach (from AD-416) Segregating populations being developed using 81 Plant Introductions (PIs) with putative resistance to soybean rust will be screened by the various collaborators to identify a set of the most resistant and a set of the most susceptible lines from each population in order to apply bulked segregant analysis (BSA). The resistant and susceptible bulks from each population, along with the parents will be screened using the Universal 1536 Soy Linkage Panel 1.1 (USLP 1.1) and analyzed on the Illumina BeadStation 500G. In those instances where resistance appears to be quantitatively inherited (slow rusting), the entire population will be tested with the USLP 1.1 in order to map the quantitative trait loci (QTL) controlling resistance. In some instances fine mapping and haplotyping of resistance loci will also be completed. Funds originated from the United Soybean Board. The specific goal of the collaborative research is to determine the genome locations of soybean rust resistance genes to allow selection of resistance in the absence of the disease and to facilitate the combining of different rust resistance genes in a single soybean variety. This research uses the 1536 single nucleotide polymorphism (SNP) DNA markers of the Universal Soybean Linkage Panel 1.0 (USLP 1.0) to analyze breeding populations in which genes for resistance to soybean rust are present. This will allow the determination of the genome positions of resistance genes and will identify DNA markers that can be used to select soybean breeding lines that carry the resistance genes. To this end, a total of 196 DNA samples from collaborators at the University of Illinois and the University of Georgia were analyzed using the USLP 1.0. The DNA samples were derived from progeny of crosses of rust susceptible soybean varieties with 23 different Plant Introductions from the USDA ARS Soybean Germplasm Collection known to carry resistance to soybean rust. Based upon these analyses, it was determined that a number of the resistance genes appear to be located at the same positions on the soybean chromosomes as previously identified resistance genes. However, a number appear to be located at new positions and thus may represent new resistance genes.
Impacts
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Progress 10/01/09 to 09/30/10
Outputs
Progress Report Objectives (from AD-416) The discovery of genes or quantitative trait loci (QTL) that control resistance in soybean to the soybean rust pathogen. Approach (from AD-416) Segregating populations being developed using 81 Plant Introductions (PIs) with putative resistance to soybean rust will be screened by the various collaborators to identify a set of the most resistant and a set of the most susceptible lines from each population in order to apply bulked segregant analysis (BSA). The resistant and susceptible bulks from each population, along with the parents will be screened using the Universal 1536 Soy Linkage Panel 1.1 (USLP 1.1) and analyzed on the Illumina BeadStation 500G. In those instances where resistance appears to be quantitatively inherited (slow rusting), the entire population will be tested with the USLP 1.1 in order to map the quantitative trait loci (QTL) controlling resistance. In some instances fine mapping and haplotyping of resistance loci will also be completed. Funds from the University of Illinois, Urbana, IL are provided by the United Soybean Board (USB), as part of USB Project #9235 entitled �Identification and Utilization of Resistance to Soybean Rust�. The specific goal of the collaborative research is to determine the genome locations of soybean rust resistance genes to allow selection of resistance in the absence of the disease and to facilitate the combining of different rust resistance genes in a single soybean variety. This research uses the 1536 single nucleotide polymorphism (SNP) DNA markers of the Universal Soybean Linkage Panel 1.0 (USLP 1.0) to analyze breeding populations segregating for soybean rust resistance in order to discover the genome positions of resistance genes. To this end a total of 480 DNA samples were analyzed using the USLP 1.0. The DNA included the parents and recombinant inbred lines of two crosses segregating for quantitative resistance to soybean rust. The two crosses were Williams 82 x DT2000 and S01-8401 x DT2000. DT2000 is a late maturing line with soybean rust resistance. All the lines from each of the two crosses had been screened for rust resistance in the field in Viet Nam by a project collaborator at the University of Missouri. Progress is monitored via quarterly written reports and by e-mail and phone communication with collaborators and the project coordinator at the University of Illinois. In addition, periodic meetings including the annual Soybean Breeder�s Workshop serve to facilitate communication between investigators and the coordinator of the project.
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