Progress 08/01/08 to 07/31/13
Outputs
Progress Report Objectives (from AD-416): The objectives of this cooperative research are: 1) develop and test wheat lines with resistance to KB; and 2) develop and test molecular markers for resistance to KB. Approach (from AD-416): Wheat experimental lines have been developed by crossing adapted varieties with different sources of resistance to KB. Wheat lines will be tested for resistance to KB in the field in India. Genetic mapping populations and near isogenic lines will be developed to identify and validate molecular markers for KB resistance genes. Molecular marker genotyping will be done in India and the USA. Data will be analyzed cooperatively. This is the final report of the project entitled �Karnal Bunt Cooperative Research� with Punjab Agricultural University. Resistance to KB was identified in several experimental hard red winter wheat lines. One line with moderate resistance to KB was released as cultivar �TAM 401� by Texas A&M University. A doubled haploid mapping population between a source of very high resistance (KRL22) and a susceptible line (WH542) was evaluated over four years at Ludhiana, Punjab, India and for three years at Obregon, Sonora, Mexico. Resistance segregated as a quantitative trait and heritability of resistance was moderately high. This population was also genotyped using the 90K wheat genotyping array. Data are being analyzed to find the locations of resistance genes for Karnal bunt as well as suitable linked molecular markers. A resistance backcrossing program using cultivar �Jagger� as the recurrent parent was carried to the fourth backcross generation. A derivative of Jagger with very good agronomic type and very good resistance to Karnal bunt was developed.
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Progress 10/01/11 to 09/30/12
Outputs
Progress Report Objectives (from AD-416): The objectives of this cooperative research are: 1) develop and test wheat lines with resistance to KB; and 2) develop and test molecular markers for resistance to KB. Approach (from AD-416): Wheat experimental lines have been developed by crossing adapted varieties with different sources of resistance to KB. Wheat lines will be tested for resistance to KB in the field in India. Genetic mapping populations and near isogenic lines will be developed to identify and validate molecular markers for KB resistance genes. Molecular marker genotyping will be done in India and the USA. Data will be analyzed cooperatively. This is the final year of the cooperative Karnal Bunt field screening project in India. Many wheat lines that showed resistance in previous years again showed moderate to good resistance in the 2012 nursery. A doubled haploid mapping population between a source of very high resistance and a susceptible line was evaluated for the fourth year. This population will be genotyped in the coming year by ARS researchers in Manhattan, KS. Data will be analyzed to find the locations of resistance genes for Karnal Bunt. A backcrossing program using Jagger as the recurrent parent is at the fourth backcross generation. We now have a derivative of Jagger with very good agronomic type and very good resistance to Karnal Bunt.
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Progress 10/01/10 to 09/30/11
Outputs
Progress Report Objectives (from AD-416) The objectives of this cooperative research are: 1) develop and test wheat lines with resistance to KB; and 2) develop and test molecular markers for resistance to KB. Approach (from AD-416) Wheat experimental lines have been developed by crossing adapted varieties with different sources of resistance to KB. Wheat lines will be tested for resistance to KB in the field in India. Genetic mapping populations and near isogenic lines will be developed to identify and validate molecular markers for KB resistance genes. Molecular marker genotyping will be done in India and the USA. Data will be analyzed cooperatively. Hard red or white winter wheats were inoculated with sporidial suspensions derived from a mixture of isolates of the Karnal Bunt pathogen (Tilletia indica) by the syringe inoculation method under field conditions in Ludhiana, Punjab, India. The 4th, 5th, 6th, 7th, and 8th Winter Wheat Karnal Bunt Screening Nurseries (WWKBSN) were vernalized and planted in October, 2010. All the lines were inoculated at the appropriate stage, i.e. at awn emergence. Frequent irrigation was carried out to maintain adequate humidity for disease development. Plants were scored in March-April, 2011. Disease pressure was uniformly high and results were consistent between replications. Many wheat lines that showed resistance in previous years again showed moderate to good resistance. A set of progeny from the cross between HD29 (resistant Indian spring wheat variety) and Jagger (susceptible Kansas winter wheat variety) showed several fixed lines with good resistance. Likewise, progeny from a cross between a resistant synthetic spring wheat and Karl 92 (susceptible Kansas winter type) continued to show moderate resistance. A new doubled haploid mapping population between a source of very high resistance and a susceptible line was evaluated for the third year. This population will be genotyped in the coming year. A backcrossing program using Jagger as the recurrent parent is at the third backcross generation. One more year of testing is needed to validate the resistance in these lines. Progress on this agreement is monitored by regularly discussing program goals, approaches, and results (teleconference, email) and by reviewing annual accomplishments reports.
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Progress 10/01/09 to 09/30/10
Outputs
Progress Report Objectives (from AD-416) The objectives of this cooperative research are: 1) develop and test wheat lines with resistance to KB; and 2) develop and test molecular markers for resistance to KB. Approach (from AD-416) Wheat experimental lines have been developed by crossing adapted varieties with different sources of resistance to KB. Wheat lines will be tested for resistance to KB in the field in India. Genetic mapping populations and near isogenic lines will be developed to identify and validate molecular markers for KB resistance genes. Molecular marker genotyping will be done in India and the USA. Data will be analyzed cooperatively. Winter wheats were inoculated with sporidial suspensions derived from a mixture of isolates of the Karnal Bunt pathogen (Tilletia indica) by the syringe inoculation method under natural field conditions in Ludhiana, Punjab, India. The 4th, 5th, 6th, and 7th Winter Wheat Karnal Bunt Screening Nurseries (WWKBSN) were vernalized and planted. All the lines could be inoculated at the appropriate stage, i.e. at awn emergence. Frequent irrigation was carried out to maintain adequate humidity for disease development. Many lines that showed resistance in previous years again showed moderate to good resistance. A set of progeny from the cross between HD29 (resistant Indian spring wheat variety) and Jagger (susceptible Kansas winter wheat variety) showed many individual with good resistance. Likewise, a cross between a resistant synthetic spring wheat and Karl 92 (susceptible Kansas winter type) continued to show moderate resistance. A new doubled haploid mapping population between a source of very high resistance and a susceptible line was evaluated for the second year. Disease incidence was low and thus the test will need to be repeated next year. Progress on this agreement is monitored by regularly discussing program goals, approaches, and results (teleconference, email) and by reviewing annual accomplishments reports.
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