Progress 04/01/08 to 03/31/11
Outputs
Progress Report Objectives (from AD-416) The objective of this research is to define the positions of the quantitative trait loci (QTL) (genes or other genetic controlling elements) that produce a breeding line with soybean oil which has at least 50% oleic acid (mid-oleic soybean) versus the normal soybean oil with only 22% oleic acid. A second objective is to identify the specific DNA markers that can be used to select breeding lines that carry the mid- oleic trait. Approach (from AD-416) A total of 960 breeding lines from populations segregating for the mid- oleic trait will be analyzed with 1536 selected SNP (single nucleotide polymorphism) DNA markers. These markers have been selected for high levels of polymorphism using 1) 96 diverse modern cultivars and 2) 96 diverse Plant Introductions from the soybean germplasm collection. The markers have also been selected to be evenly distributed across the 20 soybean linkage groups or chromosomes. Each breeding line will be analyzed with the 1536 SNP DNA markers. Collaborators will collect data on the level of fatty acids in each of the breeding lines. The analysis of the DNA marker data and the fatty acid data will allow us to associate specific DNA markers with the genes or quantitative trait loci (QTL) controlling fatty acid content in the soybean oil. The DNA markers identified in this fashion can be used in marker assisted selection to identify breeding lines with the mid-oleic trait (greater than 50% oleic acid) in the soybean oil. This is in cooperation with researchers from USDA-ARS Raleigh, NC. The research approach is to apply a set of 1536 SNP DNA markers that are highly informative in both elite U.S. soybean cultivars as well as in diverse soybean germplasm accessions from the USDA Soybean Germplasm Collection and which are well distributed across the 20 soybean chromosomes. This set of 1536 DNA markers referred to as the Universal Soybean Linkage Panel 1.0 (USLP 1.0) was used to analyze the DNA of 280 progeny and the parents derived from a cross of the soybean Williams 82 with a wild soybean, PI 366121. The population was created and the seed was analyzed for oil content and for fatty acid composition of the oil by collaborators at the USDA, ARS, Columbia, Missouri. The objective of these analyses was the discovery of genes controlling the level of seed oil and the fatty acid composition of the oil.
Impacts
(N/A)
Publications
|
Progress 10/01/09 to 09/30/10
Outputs
Progress Report Objectives (from AD-416) The objective of this research is to define the positions of the quantitative trait loci (QTL) (genes or other genetic controlling elements) that produce a breeding line with soybean oil which has at least 50% oleic acid (mid-oleic soybean) versus the normal soybean oil with only 22% oleic acid. A second objective is to identify the specific DNA markers that can be used to select breeding lines that carry the mid- oleic trait. Approach (from AD-416) A total of 960 breeding lines from populations segregating for the mid- oleic trait will be analyzed with 1536 selected SNP (single nucleotide polymorphism) DNA markers. These markers have been selected for high levels of polymorphism using 1) 96 diverse modern cultivars and 2) 96 diverse Plant Introductions from the soybean germplasm collection. The markers have also been selected to be evenly distributed across the 20 soybean linkage groups or chromosomes. Each breeding line will be analyzed with the 1536 SNP DNA markers. Collaborators will collect data on the level of fatty acids in each of the breeding lines. The analysis of the DNA marker data and the fatty acid data will allow us to associate specific DNA markers with the genes or quantitative trait loci (QTL) controlling fatty acid content in the soybean oil. The DNA markers identified in this fashion can be used in marker assisted selection to identify breeding lines with the mid-oleic trait (greater than 50% oleic acid) in the soybean oil. Funds from the USDA, ARS Raleigh, NC are provided by the United Soybean Board, as part of Project #8222 entitled �Development of Mid-Oleic, Low Linolenic, Low-Saturated Substitutes for Partially Hydrogenated Soybean Oil�. The research approach is to apply a set of 1536 SNP DNA markers that are highly informative in both elite U.S. soybean cultivars as well as in diverse soybean germplasm accessions from the USDA Soybean Germplasm Collection and which are well distributed across the 20 soybean chromosomes. This set of 1536 DNA markers now referred to as the Universal Soybean Linkage Panel 1.0 (USLP 1.0) was used to analyze DNA of 118 lines of the North Carolina RSII (RS = Recurrent Selection) population and 101 lines of the North Carolina RSIII population along with the parents of these populations.. These are two unique populations that have undergone more than 25 generations of random intermating. The populations were established to study the correlations between seed oil and protein and seed yield and other traits of importance in soybean production. The RSII population was developed using a set of soybean Plant Introductions while the RSIII population is derived from six cultivars. In each population intermating was facilitated by the use of male sterility. The DNA marker analysis of these lines will allow a better definition of the genetic control of and the interactions between soybean seed oil and protein and seed yield as well as other phenotypic traits. In addition, DNA of a total of 48 recombinant inbred lines from the University of Missouri from the cross of M23 x PI 283327 were analyzed with the 1536 SNPs of the USLP 1.0. Progress is monitored via quarterly written reports and by frequent phone conferences with the collaborator at the USDA Raleigh and via e-mail correspondence concerning the selection of populations to be analyzed with the 1536 SNP markers. In addition, periodic meetings including the annual Soybean Breeder�s Workshop serve to facilitate communication between investigators and the coordination of the project.
Impacts
(N/A)
Publications
|
Progress 10/01/08 to 09/30/09
Outputs
Progress Report Objectives (from AD-416) The objective of this research is to define the positions of the quantitative trait loci (QTL) (genes or other genetic controlling elements) that produce a breeding line with soybean oil which has at least 50% oleic acid (mid-oleic soybean) versus the normal soybean oil with only 22% oleic acid. A second objective is to identify the specific DNA markers that can be used to select breeding lines that carry the mid- oleic trait. Approach (from AD-416) A total of 960 breeding lines from populations segregating for the mid- oleic trait will be analyzed with 1536 selected SNP (single nucleotide polymorphism) DNA markers. These markers have been selected for high levels of polymorphism using 1) 96 diverse modern cultivars and 2) 96 diverse Plant Introductions from the soybean germplasm collection. The markers have also been selected to be evenly distributed across the 20 soybean linkage groups or chromosomes. Each breeding line will be analyzed with the 1536 SNP DNA markers. Collaborators will collect data on the level of fatty acids in each of the breeding lines. The analysis of the DNA marker data and the fatty acid data will allow us to associate specific DNA markers with the genes or quantitative trait loci (QTL) controlling fatty acid content in the soybean oil. The DNA markers identified in this fashion can be used in marker assisted selection to identify breeding lines with the mid-oleic trait (greater than 50% oleic acid) in the soybean oil. Significant Activities that Support Special Target Populations Funds from the USDA, ARS Raleigh, NC are provided by the United Soybean Board, as part of Project #8222 entitled �Development of Mid-Oleic, Low Linolenic, Low-Saturated Substitutes for Partially Hydrogenated Soybean Oil�. The research approach is to apply a set of 1536 SNP DNA markers that have been identified based upon their even distribution across the 20 soybean linkage groups and that also are highly informative in both elite U.S. soybean cultivars as well as in diverse soybean germplasm accessions from the USDA Soybean Germplasm Collection. This set of 1536 DNA markers now referred to as the Universal Soybean Linkage Panel 1.0 (USLP 1.0) was used to genotype 306 RILs, F1s and parents of the cross of Jake x PI 416908 which are segregating for oleic acid level in the oil. This population was received from a collaborator at the University of Missouri. A total of 515 of the 1536 SNPs were polymorphic in this population and the genotype data were forwarded to the collaborator. DNA of a total of 576 RILs and parents from the cross of N98-4445A x N97-3225 which are segregating for oleic and palmitic acid levels in the oil were received from a collaborator at North Carolina State University. These lines were analyzed with the USLP 1.0 and the genotype data were forwarded to the collaborator. In addition, DNA of 400 RILs, parents and F1�s of Jake x PI 283327 which are segregating for oleic acid level in the oil were genotyped with the USLP 1.0 and the genotype data were returned to the collaborator at the University of Missouri. Progress is monitored via quarterly written reports and by frequent phone conferences with the collaborator at the USDA Raleigh and via e-mail correspondence concerning the selection of populations to be analyzed with the 1536 SNP markers. In addition, periodic meetings including the annual Soybean Breeder�s Workshop serve to facilitate communication between investigators and the coordination of the project.
Impacts
(N/A)
Publications
|
Progress 10/01/07 to 09/30/08
Outputs
Progress Report Objectives (from AD-416) The objective of this research is to define the positions of the quantitative trait loci (QTL) (genes or other genetic controlling elements) that produce a breeding line with soybean oil which has at least 50% oleic acid (mid-oleic soybean) versus the normal soybean oil with only 22% oleic acid. A second objective is to identify the specific DNA markers that can be used to select breeding lines that carry the mid- oleic trait. Approach (from AD-416) A total of 960 breeding lines from populations segregating for the mid- oleic trait will be analyzed with 1536 selected SNP (single nucleotide polymorphism) DNA markers. These markers have been selected for high levels of polymorphism using 1) 96 diverse modern cultivars and 2) 96 diverse Plant Introductions from the soybean germplasm collection. The markers have also been selected to be evenly distributed across the 20 soybean linkage groups or chromosomes. Each breeding line will be analyzed with the 1536 SNP DNA markers. Collaborators will collect data on the level of fatty acids in each of the breeding lines. The analysis of the DNA marker data and the fatty acid data will allow us to associate specific DNA markers with the genes or quantitative trait loci (QTL) controlling fatty acid content in the soybean oil. The DNA markers identified in this fashion can be used in marker assisted selection to identify breeding lines with the mid-oleic trait (greater than 50% oleic acid) in the soybean oil. Significant Activities that Support Special Target Populations Funds from the USDA, ARS Raleigh, NC are provided by the United Soybean Board, as part of Project #8222 entitled �Development of Mid-Oleic, Low Linolenic, Low-Saturates for Partially Hydrogenated Soybean Oil�. This is a new project which is a continuation of a project whose goal is the identification of single nucleotide polymorphism (SNP) DNA markers to select for soybean breeding lines with altered fatty acid levels in the soybean oil. The research approach is to apply a set of 1536 SNP DNA markers that have been identified based upon their even distribution across the 20 soybean linkage groups and that also are highly informative in both elite U.S. soybean cultivars as well as in diverse soybean germplasm accessions from the USDA Soybean Germplasm Collection. This set of 1536 SNP markers will be analyzed on various soybean populations that are segregating for oil quality traits including the level of palmitic acid, linolenic acid, and oleic acid. A number of the collaborators on this multi-institutional project have populations that have been developed and for which phenotypic data have been or are being accumulated. DNA is being isolated from the recombinant inbred lines of these populations and is being forwarded to the USDA, Beltsville for analysis using the 1536 SNPs. Progress is monitored via quarterly written reports and by frequent phone conferences with the collaborator at the USDA Raleigh and via e-mail correspondence concerning the selection of populations to be analyzed with the 1536 SNP markers. In addition, periodic meetings including the annual Soybean Breeder�s Workshop serve to facilitate communication between investigators and the coordination of the project.
Impacts
(N/A)
Publications
|
|