Progress 04/22/08 to 02/28/11
Outputs
Progress Report Objectives (from AD-416) 1. Increase the number and types of breeding populations for combining high protein, high digestible sugar, low oligosaccharides, and low phytate in the elite background. 2. Develop new conventional and RR breeding lines with high protein, high sucrose, low stachyose, low phytate, and high yield potential. 3. Increase the yield level of the best high protein and low phytate lines to be comparable with popular commercial cultivars. 4. Release lines developed from this research project for crossing and cultivar development by other soybean breeders. Approach (from AD-416) Produce breeder and foundation seed of the high protein cultivar Osage and germplasm R95-1705 for release to public and private breeders for crossing. Evaluate yield and protein levels of new advanced lines in local tests and in the USDA regional uniform and quality traits tests. Yield test advanced and preliminary breeding lines derived from high protein crosses. Advance plant populations for development of new lines with different maturities and improved seed compositions. Construct new plant populations that combine different sources of high protein, high sucrose, low stachyose, and low phytate in elite conventional and glyphosate resistant genetic backgrounds. This project is related to Objective 2 of the in-house project: To discover novel genes/alleles in soybean for seed composition, determine their inheritance, determine genomic location, transfer to adapted germplasm, and release. High protein germplasm releases R05-1415 and R05-1772 were developed and released from the University of Arkansas in 2010. R05-1415 was developed from the cross between MFS 591 and V96-4486. R05-1415 has been evaluated in a total of 30 environments from 2006 to 2009. On average, R05-1415 yielded 94 percent of the high-yielding MGV conventional cultivar 5002T and had a protein content of 46.9 percent. R05-1772 originated from the cross between R95-1705 and V96-4181. R05-1772 has been tested in a total of 29 environments from 2006 to 2009. Average yield of R05-1772 was 91 percent of 5002T with a protein content of 46.1 percent. Both R05-1415 and R05-1772 will be excellent parents for high protein soybean breeding. The main traits that have been combined with the high protein traits are: high yield, modified sugars, large seed, small seed, low phytate, and RR. The sources of high protein used in developing our populations were lines that exhibited high protein in the Quality Traits Tests, in the USDA Uniform and Preliminary Tests, or from screening of seed requested from the USDA soybean germplasm repository. The main sources of high sugar have been PI200508, V99-5089, PI 243545, PI424444B, PI507429 and PI594268B. The low phytate trait has been incorporated into our breeding lines by using lines containing the trait derived from CX1834 or V99-5089. Low phytate lines included in our crossing block were derived from lines that performed well in the Quality Trait Tests. The Authorized Departmental Officer's Designated Representative monitored activities of project through frequent phone calls and emails, two formal meetings, site visits, and through quarterly reports.
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Progress 10/01/09 to 09/30/10
Outputs
Progress Report Objectives (from AD-416) 1. Increase the number and types of breeding populations for combining high protein, high digestible sugar, low oligosaccharides, and low phytate in the elite background. 2. Develop new conventional and RR breeding lines with high protein, high sucrose, low stachyose, low phytate, and high yield potential. 3. Increase the yield level of the best high protein and low phytate lines to be comparable with popular commercial cultivars. 4. Release lines developed from this research project for crossing and cultivar development by other soybean breeders. Approach (from AD-416) Produce breeder and foundation seed of the high protein cultivar Osage and germplasm R95-1705 for release to public and private breeders for crossing. Evaluate yield and protein levels of new advanced lines in local tests and in the USDA regional uniform and quality traits tests. Yield test advanced and preliminary breeding lines derived from high protein crosses. Advance plant populations for development of new lines with different maturities and improved seed compositions. Construct new plant populations that combine different sources of high protein, high sucrose, low stachyose, and low phytate in elite conventional and glyphosate resistant genetic backgrounds. This project is related to Objective 2 of the in-house project: To characterize the biological mechanisms that control phytic acid concentration in soybean seed, and determine the impact of genetically lowering phytic acid on seed and seedling vigor, soy protein functionality and soybean meal digestibility. Regional Quality Traits test: Seven lines with high protein were tested in the 2009 Quality Trait MG 5. Lines with good performance included R05- 1415 (MFS-591 x V96-4486), MG 5E, and R05-1746 (R95-1705 x V96-4169), MG 5M. R05-1415 yielded 92% of the check average (5002T, 5601T) across nine locations and had an average protein content of 41.6% (13% moisture) across nine locations. R05-1746 had an average yield of 92.1% of the average of 5002T and 5601T, and 41.4% protein (average of 9 locations). State high protein final test: Twelve advanced MG 4-5 high protein lines were tested at four Arkansas locations each with three reps in 2009. Yields of these lines were variable and protein content ranged from 35.5% to 40.9% (13% moisture). Four lines had yields 96% and 105% of the commercial check average and had high protein. State high protein intermediate test: Twelve MG 4-5 lines were evaluated in a 3-rep test at three locations in 2009. Yields of these lines were 66% to 99% of the check average (5601T, Osage, R95-1705) while protein ranged from 36% to 40.5% (13% moisture). Four lines yielded between 94% and 104% of the commercial checks, and had protein content between 37.9% and 39.2% (13% moisture). Low phytate lines: Thirty-three lines from low phytate and high sugar test were screened, by the inorganic phosphorous (Pi) assay, for the low phytate trait. Seven lines with V99-5089 in the pedigree exhibited inorganic phosphorous levels between CX1834-1-2 and V99-5089, and were considered low phytate. High or low sugar lines: Five advanced lines (R07-10291, R07-1573, R07- 1585, R07-15003, R07-1674) selected from 2008 sugars tests, along with sixteen high sugar genotypes were tested in a 3-rep test at two Arkansas locations in 2009. The sixteen new genotypes were derived from high protein x high sucrose, high yield x high sucrose/low stachyose and high yield x high sucrose/low stachyose cross combinations. Yield of our advanced sugar lines ranged from 37% to 97% of average yield check. A high sucrose line, R07-2000, had competitive yield to check cultivars consistently over locations, and had low phytate, high sucrose and low stachyose. Progress was monitored through phone calls and emails, two formal meetings, site visits, and through quarterly reports.
Impacts
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Progress 10/01/08 to 09/30/09
Outputs
Progress Report Objectives (from AD-416) 1. Increase the number and types of breeding populations for combining high protein, high digestible sugar, low oligosaccharides, and low phytate in the elite background. 2. Develop new conventional and RR breeding lines with high protein, high sucrose, low stachyose, low phytate, and high yield potential. 3. Increase the yield level of the best high protein and low phytate lines to be comparable with popular commercial cultivars. 4. Release lines developed from this research project for crossing and cultivar development by other soybean breeders. Approach (from AD-416) Produce breeder and foundation seed of the high protein cultivar Osage and germplasm R95-1705 for release to public and private breeders for crossing. Evaluate yield and protein levels of new advanced lines in local tests and in the USDA regional uniform and quality traits tests. Yield test advanced and preliminary breeding lines derived from high protein crosses. Advance plant populations for development of new lines with different maturities and improved seed compositions. Construct new plant populations that combine different sources of high protein, high sucrose, low stachyose, and low phytate in elite conventional and glyphosate resistant genetic backgrounds. Significant Activities that Support Special Target Populations This project is related to Objective 2 of the in-house project: To characterize the biological mechanisms that control phytic acid concentration in soybean seed, and determine the impact of genetically lowering phytic acid on seed and seedling vigor, soy protein functionality and soybean meal digestibility. 43 breeding populations were developed from different cross combinations of high-protein, low-phytate, and high/modified sugar contents and were planted in the summer of 2009. Eleven lines with high protein and one line with low phytate were tested in the 2008 Regional Quality Tests. Lines with superior performance included R05-1415 and R05-1746. In 2009, a total of seven high-protein lines were entered in the Regional Quality Traits, with four lines being re-tested and three new entries. The new entries are progeny that have the high-protein trait from either R95-1705 or CX1834-1-2. In 2008, thirty-two advanced MG 4-6 high protein lines were tested at three Arkansas locations each with three reps in 2008. Twenty-two preliminary MG 4-5 high protein lines were evaluated in a 3-rep test at two locations. Eighty-seven new lines (MG 4-6) with high protein and good agronomic traits were also tested. Sixty-one lines from low-phytate crosses (MG 4-6) were evaluated and 39 MG 4-5 lines with high sucrose or low stachyose were selected. These lines were evaluated in a preliminary 1-rep-1-loc test. 241 Plant Introductions from 28 countries were screened and 20 were identified with unique sugar profiles: high or low sucrose, high or low stachyose, high glucose, high fructose, and low raffinose. They were evaluated in a 3-rep test at 2 locations in 2008 to study the stability of sugar profile and GxE effect on sugar content. The variety �Osage� was released as a conventional cultivar with high yield (95-105% of commercial checks) and high protein (43-44%) (Chen et al., 2007; J. Plant Registration 1:89-92). A high protein line, R95-1705, was released as germplasm. R95-1705 averages 46.7% protein and 86% yield of the commercial check cultivars (Chen et al., 2008; J. Plant Registration 2:58-59). It has been used as a high protein parent by many public and private breeders in their breeding programs. Several farmers are raising R95-1705 for specialty food or feed market. Progress was monitored through frequent phone calls and emails, two formal meetings, site visits, and through quarterly reports.
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Progress 10/01/07 to 09/30/08
Outputs
Progress Report Objectives (from AD-416) 1. Increase the number and types of breeding populations for combining high protein, high digestible sugar, low oligosaccharides, and low phytate in the elite background. 2. Develop new conventional and RR breeding lines with high protein, high sucrose, low stachyose, low phytate, and high yield potential. 3. Increase the yield level of the best high protein and low phytate lines to be comparable with popular commercial cultivars. 4. Release lines developed from this research project for crossing and cultivar development by other soybean breeders. Approach (from AD-416) Produce breeder and foundation seed of the high protein cultivar Osage and germplasm R95-1705 for release to public and private breeders for crossing. Evaluate yield and protein levels of new advanced lines in local tests and in the USDA regional uniform and quality traits tests. Yield test advanced and preliminary breeding lines derived from high protein crosses. Advance plant populations for development of new lines with different maturities and improved seed compositions. Construct new plant populations that combine different sources of high protein, high sucrose, low stachyose, and low phytate in elite conventional and glyphosate resistant genetic backgrounds. Significant Activities that Support Special Target Populations This project is related to Objective 2 of the in-house project: To characterize the biological mechanisms that control phytic acid concentration in soybean seed, and determine the impact of genetically lowering phytic acid on seed and seedling vigor, soy protein functionality and soybean meal digestibility. Twenty-five sugar breeding populations derived from high sucrose, high glucose, high fructose, and low stachyose crosses, and in various stages of inbreeding, are being advanced in 2008. Forty-nine plant populations from high protein crosses with high yield, RR, and low stachyose are being advanced. Seventeen breeding populations derived from low phytate crosses with high yield or high protein parents are being advanced. Inbred progeny rows derived from 18 high protein and 11 sugar (high sucrose, low stachyose) populations are being evaluated in 2008. Nine lines with high protein and reasonable yields have been selected and entered in the 2008 Regional Quality Traits Test. The protein content of the selected lines ranged from 44.8 to 49.8% and yields ranged from 80 to 94% check yield. A low phytate line with fair yield (79.8% of 5601T) was selected and has been entered in the 2008 Regional Quality Traits Test. Twenty-two preliminary lines were selected for high protein (43-49%) and acceptable yields (above 80% of check). They are being evaluated in a 3- rep test at two locations in 2008. Seven low phytate lines were selected and are being tested at two locations with 3 reps in 2008. Fifty-four new low phytate lines were identified and they are being evaluated in a preliminary test with 1 rep at one location in 2008. The Authorized Departmental Officer's Designated Representative monitored activities of project through frequent phone calls and emails, two formal meetings, site visits, and through quarterly reports.
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