Progress 10/01/11 to 09/30/12
Outputs
Progress Report Objectives (from AD-416): The proposed objectives are: 1) to conduct on-farm surveys in organic and all natural poultry production practices to determine the prevalence and diversity of Salmonella serovars in these production systems as compared to more intensive commercial systems; 2) to develop and evaluate intervention strategies targeting control of Salmonella during the feed withdrawal and transportation processes prior to slaughter of broiler chickens to minimize cross contamination during transport and slaughter; 3) to evaluate post-harvest interventions to control Salmonella using novel antimicrobials in pre- and post-chiller applications, for both water and air chilling, and for finished raw products; and 4) to develop risk assessment models that can be adapted to organic and all natural production and processing systems. The proposed research will generate multiple publications in peer-reviewed scientific journals, presentations in meetings, and annual updates as well as a complete final report to the funding agency. Approach (from AD-416): A minimum of 10 flocks from each production system will be sampled during grow-out. Chick box paper pads from each production system will be collected from a minimum of 10 boxes and will be sampled. Each flock will be sampled on at least 3 occasions and 6-10 of each sample type will be collected during each sampling occasion. Salmonella parameters will be evaluated on each sample collected: 1) isolation frequency; 2) serotype diversity; 3) antimicrobial resistance pattern; and 4) genotype diversity. On-farm Salmonella prevalence surveys were conducted, and Salmonella enterica serovar Kentucky was the most frequent serovar found. Antimicrobial resistance genes were identified in Salmonella strains isolated both from intensive and pasture-raised poultry. The majority of the Salmonella Kentucky isolates were resistant to arsenic. Genotyping analysis revealed a high diversity and clonal distribution of Salmonella serovars in all production systems. Intervention strategies to reduce the prevalence of Salmonella have been identified, and evaluated. Prebiotics and organic acids have been shown to reduce the prevalence of Salmonella in birds. This project relates to objective 1 of the parent project. Comparing incidence of Salmonella in different production systems allowed for determining how broilers raised under different conditions of welfare (stress) respond to Salmonella infections. Additionally, this project generated information on potential intervention strategies to reduce Salmonella in broilers.
Impacts
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Publications
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Progress 10/01/10 to 09/30/11
Outputs
Progress Report Objectives (from AD-416) The proposed objectives are: 1) to conduct on-farm surveys in organic and all natural poultry production practices to determine the prevalence and diversity of Salmonella serovars in these production systems as compared to more intensive commercial systems; 2) to develop and evaluate intervention strategies targeting control of Salmonella during the feed withdrawal and transportation processes prior to slaughter of broiler chickens to minimize cross contamination during transport and slaughter; 3) to evaluate post-harvest interventions to control Salmonella using novel antimicrobials in pre- and post-chiller applications, for both water and air chilling, and for finished raw products; and 4) to develop risk assessment models that can be adapted to organic and all natural production and processing systems. The proposed research will generate multiple publications in peer-reviewed scientific journals, presentations in meetings, and annual updates as well as a complete final report to the funding agency. Approach (from AD-416) A minimum of 10 flocks from each production system will be sampled during grow-out. Chick box paper pads from each production system will be collected from a minimum of 10 boxes and will be sampled. Each flock will be sampled on at least 3 occasions and 6-10 of each sample type will be collected during each sampling occasion. Salmonella parameters will be evaluated on each sample collected: 1) isolation frequency; 2) serotype diversity; 3) antimicrobial resistance pattern; and 4) genotype diversity. On-farm Salmonella prevalence surveys have been completed. Salmonella enterica serovar Kentucky was the most commonly recovered serovar. Salmonella strains isolated from pasture-raised poultry also exhibited antimicrobial resistance phenotypes and genes. The project has been extended for an additional year to perform detailed genotyping analyses of selected strains. Several pre- and post-harvest intervention strategies have been evaluated. A combined thermal and acidified sodium propionate treatment has been identified as an effective post-harvest intervention to control Salmonella contamination of poultry products. Specific risk assessment models are currently being developed. The Principal Investigator monitors the progress of the study by coordinating frequent conference call meetings, in addition to individual meetings whenever team members attend scientific events throughout the year.
Impacts
(N/A)
Publications
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Progress 10/01/09 to 09/30/10
Outputs
Progress Report Objectives (from AD-416) The proposed objectives are: 1) to conduct on-farm surveys in organic and all natural poultry production practices to determine the prevalence and diversity of Salmonella serovars in these production systems as compared to more intensive commercial systems; 2) to develop and evaluate intervention strategies targeting control of Salmonella during the feed withdrawal and transportation processes prior to slaughter of broiler chickens to minimize cross contamination during transport and slaughter; 3) to evaluate post-harvest interventions to control Salmonella using novel antimicrobials in pre- and post-chiller applications, for both water and air chilling, and for finished raw products; and 4) to develop risk assessment models that can be adapted to organic and all natural production and processing systems. The proposed research will generate multiple publications in peer-reviewed scientific journals, presentations in meetings, and annual updates as well as a complete final report to the funding agency. Approach (from AD-416) A minimum of 10 flocks from each production system will be sampled during grow-out. Chick box paper pads from each production system will be collected from a minimum of 10 boxes and will be sampled. Each flock will be sampled on at least 3 occasions and 6-10 of each sample type will be collected during each sampling occasion. Salmonella parameters will be evaluated on each sample collected: 1) isolation frequency; 2) serotype diversity; 3) antimicrobial resistance pattern; and 4) genotype diversity. The on-farm surveys have been completed. A large collection of Salmonella has been isolated with serovar Kentucky being the most commonly recovered. Salmonella isolation data is currently being evaluated to determine the need for genotyping. Studies on addition of prebiotics to diets as a means to mitigate Salmonella colonization have been conducted, and results are currently being analyzed. Post-harvest interventions evaluation is ongoing, and final data is not available yet. Risk assessment models are currently being developed. The Principal Investigator monitors the progress of the study by coordinating frequent conference call meetings, in addition to individual meetings whenever team members attend scientific events throughout the year.
Impacts
(N/A)
Publications
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Progress 10/01/08 to 09/30/09
Outputs
Progress Report Objectives (from AD-416) The proposed objectives are: 1) to conduct on-farm surveys in organic and all natural poultry production practices to determine the prevalence and diversity of Salmonella serovars in these production systems as compared to more intensive commercial systems; 2) to develop and evaluate intervention strategies targeting control of Salmonella during the feed withdrawal and transportation processes prior to slaughter of broiler chickens to minimize cross contamination during transport and slaughter; 3) to evaluate post-harvest interventions to control Salmonella using novel antimicrobials in pre- and post-chiller applications, for both water and air chilling, and for finished raw products; and 4) to develop risk assessment models that can be adapted to organic and all natural production and processing systems. The proposed research will generate multiple publications in peer-reviewed scientific journals, presentations in meetings, and annual updates as well as a complete final report to the funding agency. Approach (from AD-416) A minimum of 10 flocks from each production system will be sampled during grow-out. Chick box paper pads from each production system will be collected from a minimum of 10 boxes and will be sampled. Each flock will be sampled on at least 3 occasions and 6-10 of each sample type will be collected during each sampling occasion. Salmonella parameters will be evaluated on each sample collected: 1) isolation frequency; 2) serotype diversity; 3) antimicrobial resistance pattern; and 4) genotype diversity. Significant Activities that Support Special Target Populations This project was initiated 1) to conduct on-farm surveys in organic and all natural poultry production practices to determine the prevalence and diversity of Salmonella serovars in these production systems as compared to more intensive commercial systems; 2) to develop and evaluate intervention strategies targeting control of Salmonella during the feed withdrawal and transportation processes prior to slaughter of broiler chickens to minimize cross contamination during transport and slaughter; 3) to evaluate post-harvest interventions to control Salmonella using novel antimicrobials in pre- and post-chiller applications, for both water and air chilling, and for finished raw products; and 4) to develop assessment models that can be adapted to organic and all natural production and processing systems. The on-farm surveys have been conducted over the last months, and should be completed by the end of the year. Upon completion of the on-farm surveys, Salmonella isolation and serotyping data will be evaluated to determine the need for genotyping. The P.I. monitors the progress of the study by coordinating frequent conference call meetings, in addition to individual meetings whenever team members attend scientific events throughout the year.
Impacts
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