Source: UNIVERSITY OF NEW HAMPSHIRE submitted to NRP
SCREENING FOR VERTICILLIUM WILT RESISTANCE IN WILD DIPLOID AND OCTOPLOID STRAWBERRY GERMPLASM
Sponsoring Institution
Agricultural Research Service/USDA
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
0412275
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
Sep 28, 2007
Project End Date
Aug 1, 2009
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
UNIVERSITY OF NEW HAMPSHIRE
51 COLLEGE RD SERVICE BLDG 107
DURHAM,NH 03824
Performing Department
PLANT BIOLOGY
Non Technical Summary
(N/A)
Animal Health Component
90%
Research Effort Categories
Basic
10%
Applied
90%
Developmental
0%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
20211221080100%
Knowledge Area
202 - Plant Genetic Resources;

Subject Of Investigation
1122 - Strawberry;

Field Of Science
1080 - Genetics;
Goals / Objectives
The objective of this cooperative research project is to screen sixty strawberry plants for susceptability to wilt. Upon completion, all pertinent data will be entered into the GRIN system so that it will be made available to others.
Project Methods
Sixty strawberry plants will be examined. Thirty-eight members of the strawberry super core, diverse diploid species, and representative octoploid strawberries will be inocculated with Verticiulium dahliae. Resistance and susceptability will be determined. Documents SCA with U. of New Hampshire. Formerly 5358-21000-033-08S (5/08).

Progress 09/28/07 to 08/01/09

Outputs
Progress Report Objectives (from AD-416) The objective of this cooperative research project is to screen sixty strawberry plants for susceptability to wilt. Upon completion, all pertinent data will be entered into the GRIN system so that it will be made available to others. Approach (from AD-416) Sixty strawberry plants will be examined in total including 38 members of the strawberry super core, diverse diploid species, and representative octoploid strawberries will be inoculated with Verticiulium dahliae. Resistance and susceptability will be determined. Documents SCA with U. of New Hampshire. Formerly 5358-21000-033-08S (5/08). Significant Activities that Support Special Target Populations The main CRIS project has responsibility for preserving and evaluating strawberry genetic resources. This specific cooperative agreement has evaluated wild strawberries with 2 sets of chromosomes and with 8 sets of chromosomes for resistance to the wilt disease called �verticillium.� The scientific progress was monitored by discussions through email, on the phone, and at professional meetings. Sixty strawberry cultivars and breeding lines were screened and rated for response to specific strains of wilt disease. Rooted runner plants were dipped in a solution of the wilt disease and were replanted. The plants were evaluated and compared to untreated plants. The strawberries varied in their responses to the disease. Some of the wild strawberries from Mendocino area, CA, and British Columbia were quite resistant, while those from Del Norte County, CA and other accessions from British Columbia and New Hampshire (�Pawtuckaway�) were highly susceptible. The resistant strawberries were donated to the National Clonal Germplasm Repository, Corvallis, OR. Among accessions collected in Hokkaido, Japan, five were intermediate. One species from Japan was difficult to screen because they were sensitive to powdery mildew. For the strawberries with 8 sets of chromosomes, �Tristar� was the most resistant tested, and �Evangeline� the most susceptible. Disease responses ranged from susceptible to highly resistant among the wild parental types of the cultivated hybrid strawberry. We have initiated resistant x susceptible crosses as an initial step toward identifying resistance genes. Results for all rated USDA-NCGR strawberries have been entered into the GRIN database working collaboratively with the Corvallis Repository Staff. The project funds have been completely used and the project has been terminated.

Impacts
(N/A)

Publications