ENUMERATION OF SALMONELLA THROUGHOUT THE PORK HARVESTING PROCESS

Recipient Organization
AGRICULTURAL RESEARCH SERVICE
(N/A)
CLAY CENTER,NE 68933

Performing Department
(N/A)

Non Technical Summary
(N/A)

Animal Health Component

80%

Research Effort Categories

Basic

20%

Applied

80%

Developmental

Classification

Knowledge Area (KA)	Subject of Investigation (SOI)	Field of Science (FOS)	Percent
712	3520	1100	100%

Knowledge Area
712 - Protect Food from Contamination by Pathogenic Microorganisms, Parasites, and Naturally Occurring Toxins;

Subject Of Investigation
3520 - Meat, swine;

Field Of Science
1100 - Bacteriology;

Keywords

Goals / Objectives
1) This research will quantify the level of Salmonella on hogs at the following stages of processing: a) skin soon after stunning and exsanguination, b) post-singed carcasses, and c) final carcasses. 2) Each sample will be assayed to determine prevalence of Salmonella. 3) A positive Salmonella sample will be serotyped and the antibiotic susceptibilities will be determined.

Project Methods
Sample collection: During each season (Summer, Fall, Winter, and Spring), 200 carcasses will be randomly sampled using a prewetted sponge at each of three sampling sites (skin soon after stunning and exsanguination, post-singeing carcasses, and final carcasses). All samples will be enumerated for Salmonella using spiral plate enumeration for skin samples and Hydrophobic Grid Membrane Analysis for post-singed and final carcasses. Sponge samples from all three sampling locations within each plant will be enriched and tested for the presence of Salmonella to determine prevalence. The serotypes of each Salmonella isolate from skin, post-singed, and final carcasses will be determined using PCR and antisera for specific O groups and flagellar antigens. The Salmonella isolated will be tested to determine the antibiotic susceptibility profile according to National Anitmicrobial/Antibiotic Resistance Monitoring System (NARMS) protocols using Sensitire antimicrobial susceptibility plates (CMV1AGNF).

Progress 05/01/07 to 10/31/08

Outputs
Progress Report Objectives (from AD-416) 1) This research will quantify the level of Salmonella on hogs at the following stages of processing: a) skin soon after stunning and exsanguination, b) post-singed carcasses, and c) final carcasses. 2) Each sample will be assayed to determine prevalence of Salmonella. 3) A positive Salmonella sample will be serotyped and the antibiotic susceptibilities will be determined. Approach (from AD-416) Sample collection: During each season (Summer, Fall, Winter, and Spring), 200 carcasses will be randomly sampled using a prewetted sponge at each of three sampling sites (skin soon after stunning and exsanguination, post-singeing carcasses, and final carcasses). All samples will be enumerated for Salmonella using spiral plate enumeration for skin samples and Hydrophobic Grid Membrane Analysis for post-singed and final carcasses. Sponge samples from all three sampling locations within each plant will be enriched and tested for the presence of Salmonella to determine prevalence. The serotypes of each Salmonella isolate from skin, post-singed, and final carcasses will be determined using PCR and antisera for specific O groups and flagellar antigens. The Salmonella isolated will be tested to determine the antibiotic susceptibility profile according to National Anitmicrobial/Antibiotic Resistance Monitoring System (NARMS) protocols using Sensitire antimicrobial susceptibility plates (CMV1AGNF). Significant Activities that Support Special Target Populations Research on this project was completed on 10/31/2008. See the annual report for FY 2008 (October 31, 2007-September 30, 2008) which details the progress of this agreement. Administrative close-out procedures have now been completed.

Impacts
(N/A)

Publications