Progress 09/15/06 to 03/14/08
Outputs
Progress Report Objectives (from AD-416) Conduct pilot study to 1) Determine beta-carotene response kinetics following a meal of golden rice; 2) Determine retinol kinetics from the golden rice and from the labeled vitamin A dose; and 3) Determine vitamin A value of golden rice beta-carotene in adults with normal or marginal vitamin A status. Approach (from AD-416) Use high performance liquid chromatography (HPLC), gas chromatography/electron capture negative chemical ionization mass spectrometry (GC/ECNCI-MS, to measure retinol enrichment in the circulation), and liquid chromatography/mass spectrometry (LC/MS) to determine the absorption and conversion of beta-Carotene from golden rice after consumption of cooked golden rice. Significant Activities that Support Special Target Populations Our investigations of bioavailability, bioconversion, antioxidant function, and tissue distribution of dietary carotenoids will continue to improve our understanding of dietary carotenoids and human health. Genetically engineered �Golden Rice� contains up to 35 micrograms of beta- carotene in a gram of dry rice. It is important to determine the vitamin A equivalency of Golden Rice beta-carotene in order to project the potential impact of this biofortified grain in rice consuming populations that commonly exhibit low vitamin A status. Golden Rice doses containing 0.99 mg � 1.53 mg of beta-carotene provided from 0.24 to 0.94 mg retinol in 5 adult volunteers (F=3, M=2). Our results show that the conversion factor for Golden Rice beta-carotene to retinol is 3.8 � 1.7 (Mean � SD) to 1 with a range of 1.9 - 6.4 to 1 by weight, or 2.0 � 0.9 to 1 with a range of 1.0 � 3.4 to 1 by moles. This project is now complete and will be terminated through the annual report process.
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Progress 10/01/06 to 09/30/07
Outputs
Progress Report Objectives (from AD-416) Conduct pilot study to 1) Determine beta-carotene response kinetics following a meal of golden rice; 2) Determine retinol kinetics from the golden rice and from the labeled vitamin A dose; and 3) Determine vitamin A value of golden rice beta-carotene in adults with normal or marginal vitamin A status. Approach (from AD-416) Use high performance liquid chromatography (HPLC), gas chromatography/electron capture negative chemical ionization mass spectrometry (GC/ECNCI-MS, to measure retinol enrichment in the circulation), and liquid chromatography/mass spectrometry (LC/MS) to determine the absorption and conversion of beta-Carotene from golden rice after consumption of cooked golden rice. Significant Activities that Support Special Target Populations This report serves to document work conducted to determine Vitamin A Value of Golden Rice with funds originating from an Interagency Agreement with US Agency for International Development(USAID)and ARS, Agreement number 60-0210-7-400, Project number 0210-22310-002-84R. The research is being carried out in this project by Tufts University investigators in the USDA Human Nutrition Research Center on Aging under an Assistance Type Cooperative Agreement #58-1950-6-656. The project is being monitored by ARS via onsite discussions with the Tufts investigator who is in regular communication with ODS and annual financial and performance reports to ARS. Additional details of research can be found in the report for the in-house associated project 1950-51000-065-00D, Dietary Antioxidants, Aging and Oxidative Stress Status. We are conducting the study to determine vitamin A value of Golden Rice in the US adults with normal vitamin A status using the Golden Rice grown and harvested from 25 atom % heavy water. Through lab analysis, the enrichment pattern of the rice beta-carotene was at M+10, which is perfectly labeled for our human study use. Each cooked dose of Golden Rice provides 1 mg of beta-carotene. Till now, four subjects have been recruited. The volunteer study will be finished by the end of the fiscal year and the sample analysis will be finished in 3 to 5 months. Our preliminary results showed that Golden Rice has the best bio-available beta-carotene and can most effectively provide vitamin A for humans.
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