Source: AGRICULTURAL RESEARCH SERVICE submitted to NRP
DEVELOPING ENHANCED SUGARBEET GERMPLASM AND MANAGEMENT APPROACHES TO INCREASE YIELD AND REDUCE LOSSES CAUSED BY PATHOGENS
Sponsoring Institution
Agricultural Research Service/USDA
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
0410945
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
Jun 20, 2006
Project End Date
Jun 1, 2008
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
AGRICULTURAL RESEARCH SERVICE
(N/A)
KIMBERLY,ID 83341
Performing Department
(N/A)
Non Technical Summary
(N/A)
Animal Health Component
50%
Research Effort Categories
Basic
50%
Applied
50%
Developmental
0%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
20120101080100%
Knowledge Area
201 - Plant Genome, Genetics, and Genetic Mechanisms;

Subject Of Investigation
2010 - Sugar beet;

Field Of Science
1080 - Genetics;
Goals / Objectives
The sugarbeet project at Kimberly, ID is focused on developing elite germplasm with enhanced disease resistance and agronomic qualities and improving disease Management options. We will evaluate, characterize, and use available genetic resources to identify resistance genes of interest. Public molecular markers linked to these new sources of resistance will be established. New sources of resistance will be incorporated into elite germplasm using traditional crossing techniques with the aid of molecular markers. Germplasm will be enhanced through the identification of novel sources of disease resistance, crossing to incorporate the new sources of resistance, and the development of markers for genes of interest to facilitate crossing and selection. To ensure that the resistance genes established for a particular pathogen are effective against all strains of the pathogen, population studies will be conducted on root rot organisms. Isolates of root rot organisms will be characterized to establish the extent of variation in the population and improve screening procedures and disease management options. We will also determine the effects of Beet necrotic yellow vein virus and Beet severe curly top virus on sugarbeet post-harvest quality and storability. The germplasm and information generated will lead to enhanced disease resistance and improved disease management options for stakeholders and advance our understanding of these disease problems in the scientific community.
Project Methods
Establish a sugarbeet research program in genetics and plant pathology using classical and molecular approaches to improve the sugarbeet genetics pool and disease management options, and enhance crop quality, productivity, yield, and profitability of production while reducing resource inputs such as fertilizer, irrigation and energy. FY06 program increase $650,628 (NTL).

Progress 06/20/06 to 06/01/08

Outputs
Progress Report Objectives (from AD-416) The sugarbeet project at Kimberly, ID is focused on developing elite germplasm with enhanced disease resistance and agronomic qualities and improving disease Management options. We will evaluate, characterize, and use available genetic resources to identify resistance genes of interest. Public molecular markers linked to these new sources of resistance will be established. New sources of resistance will be incorporated into elite germplasm using traditional crossing techniques with the aid of molecular markers. Germplasm will be enhanced through the identification of novel sources of disease resistance, crossing to incorporate the new sources of resistance, and the development of markers for genes of interest to facilitate crossing and selection. To ensure that the resistance genes established for a particular pathogen are effective against all strains of the pathogen, population studies will be conducted on root rot organisms. Isolates of root rot organisms will be characterized to establish the extent of variation in the population and improve screening procedures and disease management options. We will also determine the effects of Beet necrotic yellow vein virus and Beet severe curly top virus on sugarbeet post-harvest quality and storability. The germplasm and information generated will lead to enhanced disease resistance and improved disease management options for stakeholders and advance our understanding of these disease problems in the scientific community. Approach (from AD-416) Establish a sugarbeet research program in genetics and plant pathology using classical and molecular approaches to improve the sugarbeet genetics pool and disease management options, and enhance crop quality, productivity, yield, and profitability of production while reducing resource inputs such as fertilizer, irrigation and energy. FY06 program increase $650,628 (NTL). Significant Activities that Support Special Target Populations Objective 1. The single nucleotide polymorphic (SNP) data were incorporated into the linkage map created by the Sugarbeet and Bean Research Unit, East Lansing, MI, and made available to the public. Development of a molecular marker for Rz2 was hindered by a technical vacancy in the genetic program. Development of a marker linked to Pectobacterium resistance was dropped since recent research at the NWISRL has shown Leuconostoc is the primary bacterial pathogen of concern. This research relates to National Program 301 Component II-Crop Informatics, Genomics, and Genetic Analyses, Problem Statement IIc-Genetic Analyses and Mapping of Important Traits. Objective 2. Work to screen germplasm for novel sources of resistance to rhizomania and curly top was promising and additional research is in progress based on these initial screenings. This research relates to National Program 301 Component III-Genetic Improvement of Crops, Problem Statement IIIc-Germplasm Enhancement/Release of Improved Genetic Resources and Varieties and National Program 303 Component III-Plant Disease Resistance, Problem Statement IIIb-Disease Resistance in New Germplasm and Varieties. Objective 3. The surveys for both fungal and bacterial root rot have been completed. The bacterial portion of the survey was recently published and a publication for the fungal portion of the project is being prepared. Isolates for the Rhizoctonia project have been collected, pathogenicity tests on sugarbeet have been completed and DNA has been isolated. The genetic diversity work remains to be completed. This research relates to National Program 303 Component I-Disease Diagnosis Detection, Identification, and Characterization of Plant Pathogens, Problem Statement Ib-Detection, Identification, Characterization, and Classification of Pathogens. Objective 4. The second year of storage work for rhizomania and curly top was successfully completed and published. This research relates to National Program 303 Component II-Biology, Ecology, Epidemiology, and Spread of Plant Pathogens and Their Relationships with Hosts and Vectors, Problem Statement IIc-Population Dynamics, Spread, and Epidemiology of Pathogens.

Impacts
(N/A)

Publications

  • Strausbaugh, C.A., Rearick, E., Camp, S. 2008. Influence of curly top and Poncho Beta on sugar beet storability. Journal of Sugarbeet Research. 45(1- 2):31-47.
  • Strausbaugh, C.A., Gillen, A.M., Camp, S., Shock, C.C., Eldredge, E.P., Gallian, J.J. 2007. Relationship of beet curly top foliar ratings to sugar beet yield. Plant Disease. 91:1459-1463.
  • Strausbaugh, C.A., Gillen, A.M. 2008. Bacteria and yeast associated with sugar beet root rot at harvest in the Intermountain West. Plant Disease. 92:357-363.
  • Strausbaugh, C.A., Rearick, E., Camp, S., Gallian, J.J., Dyer, A.T. 2008. Influence of beet necrotic yellow vein virus on sugar beet storability. Plant Disease. 92:581-587.
  • Strausbaugh, C.A., Eujayl, I.A., Rearick, E., Foote, P. 2008. Sugar beet cultivars evaluated for storability in Idaho during the 2006/2007 storage season. Plant Disease Management Reports. 2:FC022.


Progress 10/01/06 to 09/30/07

Outputs
Progress Report Objectives (from AD-416) The sugarbeet project at Kimberly, ID is focused on developing elite germplasm with enhanced disease resistance and agronomic qualities and improving disease Management options. We will evaluate, characterize, and use available genetic resources to identify resistance genes of interest. Public molecular markers linked to these new sources of resistance will be established. New sources of resistance will be incorporated into elite germplasm using traditional crossing techniques with the aid of molecular markers. Germplasm will be enhanced through the identification of novel sources of disease resistance, crossing to incorporate the new sources of resistance, and the development of markers for genes of interest to facilitate crossing and selection. To ensure that the resistance genes established for a particular pathogen are effective against all strains of the pathogen, population studies will be conducted on root rot organisms. Isolates of root rot organisms will be characterized to establish the extent of variation in the population and improve screening procedures and disease management options. We will also determine the effects of Beet necrotic yellow vein virus and Beet severe curly top virus on sugarbeet post-harvest quality and storability. The germplasm and information generated will lead to enhanced disease resistance and improved disease management options for stakeholders and advance our understanding of these disease problems in the scientific community. Approach (from AD-416) Establish a sugarbeet research program in genetics and plant pathology using classical and molecular approaches to improve the sugarbeet genetics pool and disease management options, and enhance crop quality, productivity, yield, and profitability of production while reducing resource inputs such as fertilizer, irrigation and energy. FY06 program increase $650,628 (NTL). Accomplishments Influence of Beet Necrotic Yellow Vein Virus (BNYVV) on Sugarbeet Storability. Rhizomania caused by BNYVV causes severe yield losses in sugarbeets, but the influence of BNYVV on storability had not been investigated. Sugarbeets from six cultivars were harvested from BNYVV infested and disease free fields to determine the influence of BNYVV on storability. Data were collected by Agricultural Research Service (ARS) Northwest Irrigation and Soils Research Laboratory (NWISRL) personnel in Kimberly, ID, with the cooperation of personnel from the University of Idaho, Amalgamated Sugar Co., and Amalgamated Research, Inc. Storing sugarbeets infested with BNYVV was found to be costing the sugarbeet industry millions of dollars in lost sugar. Based on these data and future research to establish cultivar selection criteria, these losses can be reduced significantly. (National Program 303 Component 2-Biology, Ecology, Epidemiology, and Spread of Plant Pathogens and Their Relationships with Hosts and Vectors, Problem Statement 2C-Population Dynamics, Spread, and Epidemiology of Pathogens) Bacteria and Yeast Associated with Sugarbeet Root Rot. A previously undescribed bacterial like complex was found to be leading to sugarbeet breakdown in the field. An initial survey established the presence of a rot complex in the field. This complex was confirmed through isolations, identification tests, and pathogenicity tests to be caused by a number of bacteria and yeast species. ARS NWISRL personnel in Kimberly, ID, conducted this research in cooperation with Amalgamated Sugar Company personnel. Based on this initial research, Leuconostoc was established as the primary causal agent. Future work will focus on establishing resistance and cultivar selection criteria for this rot pathogen. (National Program 303 Component 1-Disease Diagnosis: Detection, Identification, and Characterization of Plant Pathogens, Problem Statement 1B-Detection, Identification, Characterization, and Classification of Pathogens) Relationship of Curly Top Foliar Ratings to Yield in Commercial Sugarbeet Fields. Curly top is a viral disease problem that almost eliminated the sugarbeet industry in the western United States until resistant cultivars became available. However, the link between changes in foliar disease ratings for curly top and yield in commercial fields was not established. ARS NWISRL personnel in Kimberly, ID, investigated this relationship with the cooperation of Amalgamated Sugar Company, University of Idaho, and Oregon State University personnel. Data show that with moderate disease pressure, every unit decrease in rating will result in a 2.6 to 3.1 tons per acre increase in root yield. (National Program 303 Component 2-Biology, Ecology, Epidemiology, and Spread of Plant Pathogens and Their Relationships with Hosts and Vectors, Problem Statement 2C-Population Dynamics, Spread, and Epidemiology of Pathogens) Technology Transfer Number of Active CRADAS and MTAS: 3 Number of Newspaper Articles,Presentations for NonScience Audiences: 13

Impacts
(N/A)

Publications

  • Gillen, A.M., Novy, R.G. 2007. Molecular characterization of the progeny of solanum etuberosum identifies a genomic region associated with resistance to potato leafroll virus. Euphytica. 155(3):403-415.
  • Gillen, A.M., Strausbaugh, C.A., Gallian, J. 2007. USDA-ARS sugar beet germplasm developed in Salinas, CA, evaluated for rhizomania resistance in Idaho, 2005. Plant Disease Management Reports. 1:FC053.
  • Strausbaugh, C.A., Camp, S. 2007. Verticillium wilt in experimental sugar beet cultivars in Cassia County, ID, 2006. Plant Disease Management Reports. 1:V113.
  • Strausbaugh, C.A., Camp, S. 2007. Verticillium wilt in transgenic sugar beet cultivars in Cassia County, ID, 2006. Plant Disease Management Reports. 1:V114.
  • Strausbaugh, C.A., Gillen, A.M., Gallian, J.J., Camp, S., Stander, J.R. 2006. Influence of host resistance and insecticide seed treatments on curly top in sugar beets. Plant Disease. 90(12):1539-1544.
  • Strausbaugh, C.A., Windes, J.M. 2006. Influence of subsoiling and direct seeded cereals in southeastern Idaho. Canadian Journal of Plant Pathology. 28:596-608.
  • Singh, S.P., Teran, H., Lema, M., Webster, D.M., Strausbaugh, C.A., Miklas, P.N., Schwartz, H.F., Brick, M.A. 2007. Seventy-five years of breeding dry bean of the Western USA. Crop Science. 47:981-989.
  • Strausbaugh, C.A., Camp, S. 2007. Verticillium wilt in commercial sugar beet cultivars in Cassia County, ID, 2006. Plant Disease Management Reports. 1:V112.


Progress 10/01/05 to 09/30/06

Outputs
Progress Report 1. What major problem or issue is being resolved and how are you resolving it (summarize project aims and objectives)? How serious is the problem? Why does it matter? Rhizomania, Rhizoctonia root rot, and curly top can seriously reduce sugar beet yield. Additional losses have been attributed to bacterial vascular necrosis and rot and Fusarium related problems. These disease problems not only lead to losses in the field but are likely to lead to additional losses in storage. This research project uses traditional plant breeding and plant pathology techniques, molecular biology, and biotechnological methods to improve disease management options and develop sugar beet germplasm with enhanced disease resistance, superior agronomic qualities, and increased suitability for sustainable production practices. Outside of uncontrollable losses due to weather, diseases account for the majority of economic losses to sugar beet producers across the U.S. Annual losses from rhizomania, Rhizoctonia root rot, Cercospora leaf spot and curly top are estimated to be in the tens of millions of dollars. In some cases chemical controls are available; however, they are more costly and less environmentally friendly than resistant varieties. This research contributes directly to ARS National Program (NP) 301, Plant, Microbial and Insect Genetic Resources, Genomics and Genetic Improvement, and National Program 303, Plant Diseases. 2. List by year the currently approved milestones (indicators of research progress) Northwest Irrigation and Soils Research (NWISRL) scientists developed goals and objectives which have been summarized in the prospectus that was accepted by National Program Staff in July 2005. The project plan was written and then reviewed by an Ad-Hoc Committee. The project plan and milestones were approved April 17, 2006. Year 1 (FY 2006) Establish single nucleotide polymorphism (SNP) markers on the public sugar beet map and publish manuscript. Establish SNP marker linked to cyst nematode resistance. Phenotype germplasm and initiate crossing to establish markers for resistance genes associated with resistance to rhizomania and bacterial vascular necrosis and rot. Initiate crossing to develop agronomically superior germplasm with enhanced resistance to rhizomania, curly top, and bacterial vascular necrosis and rot. Collect, isolate, identify, and initiate pathogenicity tests on sugarbeet root rot organisms to determine their importance to root health. Initiate studies to determine the importance of rhizomania and curly top on the storability of sugar beets. Year 2 (FY 2007) Develop advanced genetic populations to establish markers for resistance genes associated with resistance to rhizomania. Screen progeny and develop marker for bacterial vascular necrosis and rot. Submit manuscript on cyst nematode resistance marker. Begin screening of progeny from crosses to develop agronomically superior germplasm with enhanced resistance to rhizomania, curly top, and bacterial vascular necrosis and rot. Repeat previous years work to collect, isolate, identify, and initiate pathogenicity tests on sugarbeet root rot organisms to determine their importance to root health. Repeat studies to determine the importance of rhizomania and curly top on the storability of sugar beets. Year 3 (FY 2008) Begin disease screening of rhizomania genetic populations to establish marker associated with resistance. Continue marker development for bacterial vascular necrosis and rot. Begin intercrossing to develop agronomically superior germplasm with enhanced resistance to rhizomania, curly top, and bacterial vascular necrosis and rot. Submit manuscript on the importance of sugar beet root rot organisms on root health. Complete genetic diversity and pathogenicity work with Rhizoctonia isolates. Submit manuscript on the importance of rhizomania and curly top on the storability of sugar beets. Year 4 (FY 2009) Begin marker development with the rhizomania genetic populations to establish marker associated with resistance. Submit manuscript on marker for bacterial vascular necrosis and rot. Screen populations to develop agronomically superior germplasm with enhanced resistance to rhizomania, curly top, and bacterial vascular necrosis and rot. Submit manuscript on genetic diversity and pathogenicity work with Rhizoctonia isolates. Year 5 (FY 2010) Submit manuscript on marker associated with rhizomania resistance. Screen populations to develop agronomically superior germplasm with enhanced resistance to rhizomania, curly top, and bacterial necrosis and rot. 4a List the single most significant research accomplishment during FY 2006. Causal Agents of Sugar Beet Root Rot in the Intermountain West. Studies were conducted to identify the primary organisms responsible for sugar beet root rot in the Intermountain West (IMW). Rhizoctonia has traditionally been considered the primary cause of root rot in the IMW, although cultivars with resistance to this pathogen have not performed well. Thus, studies into the primary causal agents of sugar beet root rot in the IMW have been initiated through collaborative work between NWISRL scientists, Amalgamated Sugar Company, and Beet Sugar Development Foundation. Sugar beet roots were sampled from piling grounds throughout the IMW and the causal agents associated with root rot were identified. These data will lead to a better understanding of root rot in the IMW which will allow us to improve disease management options and guide plant breeding strategies (accomplishment aligns with NP 303 Plant Disease Component 1; Disease Diagnosis: Detection, Identification, and Characterization). 4b List other significant research accomplishment(s), if any. Influence of host resistance and insecticide seed treatments on curly top in sugar beets. Curly top on sugar beets caused by Beet severe curly top virus (BSCTV) or closely related strains is a considerable problem in arid growing regions of the western United States. Insecticide seed treatments, Poncho Beta and Gaucho, and sugar beet hybrids varying in curly top resistance were evaluated for their influence on the control of curly top. NWISRL scientists in cooperation with Amalgamated Sugar Company and University of Idaho established plots at two locations in southern Idaho with exposure to moderate to severe curly top. Poncho Beta provided a level of control that would justify its application as a supplement to host resistance under Idaho conditions (accomplishment aligns with NP 303 Plant Diseases Component 4: Biological and Cultural Strategies for Sustainable Disease Management). Based on this research, the Oregon Department of Agriculture received a Section 18 Crisis exemption for Poncho on January 27, 2006, which allows for the use of Poncho as a sugar beet seed treatment to control the leaf hopper vector of Beet severe curly top virus (BSCTV) in the following states: Colorado, Idaho, Montana, Oregon, Washington, and Wyoming. Rhizomania and Curly Top Nurseries Over 3200 plots were established in Kimberly, ID, with exposure to Beet severe curly top virus (BSCTV) and 962 plots in Burley, ID, with exposure to Beet necrotic yellow vein virus (BNYVV), the causal agent of rhizomania, to facilitate disease screening for novel sources of disease resistance. Disease control for curly top and rhizomania is primarily achieved through the use of host resistance. Our first year rhizomania results showed that the strains of rhizomania in Idaho are capable of partially defeating the Rz1 gene, which is widely deployed commercially, and that the Rz2 gene will increase effectiveness of control, but these genes alone are not enough. These data establish the urgency of finding new sources of resistance and the necessity of doing screening in Idaho in fields with the new more severe strains of the virus. In order to identify new sources of resistance, disease screening of Beta germplasm will be conducted in these fields through a collaborative effort between NWISRL scientists, Amalgamated Sugar Company, and Beet Sugar Development Foundation. These efforts will likely lead to novel sources of disease resistance for the control of curly top and rhizomania on sugar beets (accomplishment aligns with NP 301 Plant, Microbial, and Insect Genetic Resources, Genomics, and Genetic Improvement Component 3: Genetic Improvement of Crops and NP 303 Plant Disease Component 3: Plant Disease Resistance). 4d Progress report. Current projects include: Curly Top Nursery manage the nursery for the Beet Sugar Development Foundation (BSDF). Screen segregating population obtained from Agricultural Research Service (ARS) Salinas, CA, as potential population for quantitative trait loci (QTL) analysis and population development. Screen Beta corolliflora (wild relative of sugarbeet) monsomic addition lines for potential introgression of resistance into B. vulgaris background. Rhizomania Nursery Inoculated 20 acres with soil infected with a new strain of Beet necrotic yellow vein virus (BNYVV; the causal agent of rhizomania). Currently growing susceptible sugar beets to increase level of inoculum so this field can be used for screening in the future. Screening research materials from ARS Salinas, CA, and ARS East Lansing, MI, and the original source population for the Rz2 gene (C48) in a growers field in cooperation with Amalgamated Sugar Company and support from Beet Sugar Development Foundation. Concurrently the California materials are being screened in California for yield, which will give and interesting comparison. Rhizomania molecular markers Obtained polymerase chain reaction (PCR) based markers for Rz1 gene from an industry source which are subject to a material transfer agreement (MTA). Screening population C48 for potential parents for selfing and future screening for parents for genetic crosses for marker development. PCR detection of curly top virus strains Industry requested that we test samples from the western United States for the presence of the three virus species associated with curly top. We obtained PCR based markers for the species from ARS Salinas, CA, and have had a summer student working almost exclusively on optimizing and testing the markers. We are developing our own internal standard using the internal transcribed spacer (ITS) region of the Beta genome as a check for the PCR reaction. Then we will use these to screen 120 samples sent by industry in July and August 2006. In addition, a subsample of the the B. corolliflora plants that were grown in the curly top nursery in 2005 will be screened. Genetic linkage mapping Screening multiplexed SNP markers on mapping a population from ARS East Lansing, MI, will be finished this summer. We will send the data to ARS East Lansing, MI, for addition to the public sugar beet linkage map. Population development - Arranged for ARS Salinas, CA, to cross selected lines to produce base populations that combine his best materials for curly top and rhizomania resistance. We plan to eventually have the facilities to conduct these crosses ourselves. Will random mate populations, and add new materials and select, as they become available. Inheritance of resistance to Erwinia (syn. Pectobacterium) and marker development. Screened parental lines for resistance/susceptibility and selected parents from half-beets in cold storage. Prescreen of lines showed clear segregation for high levels of resistance in various lines. Crossing is being done this summer (2006) and F1 populations will be screened next year. Preliminary marker work could be done this fall using segregants from initial populations screened in Fall 2005. Beta corolliflora as a new source of very high levels of curly top resistance. The 14 accessions screened in the curly top nursery in 2005 showed no symptoms of curly top. We dug up one replication of the trial and are maintaining the plants in the greenhouse for further investigations. We performed a leafhopper choice assay in collaboration with a University of Idaho Entomologist to determine if leaf hoppers, the vector of Beet severe curly top virus, are adverse to B. corolliflora. The plants will be put into cold storage in an attempt to get seed from them next spring. Growing sugar beets in two locations for a study of the effects of curly top and rhizomania on sugar composition and sugar content of beets during storage in piles over the winter. Studying the interaction of seed treatments and host resistance with cultivars that vary for resistance to curly top to improve management options for curly top. Conducting a survey of root rot organisms in the Intermountain West region to determine the importance of root rot organisms to sugar beet root health and product quality. 5. Describe the major accomplishments to date and their predicted or actual impact. We have prepared a Prospectus and have submitted a Project Plan based on input from sugar beet scientists and industry representatives. Through achieving the objectives in this plan, this research program will develop elite germplasm with enhanced disease resistance and agronomic qualities and improve disease management options for sugar beets. These efforts align with NP 301 Component II - Genome Characterization and Genetic Improvement; Problem Areas IIa Genome Characterization and IIb - Genetic Improvement and NP 303 Component I - Identification and Classification of Pathogens and Component IV - Pathogen Biology, Genetics, Population Dynamics, Spread, and Relationship with Hosts and Vectors. We have conducted research establishing Poncho as an effective insecticide seed treatment which will supplement host resistance for the control of curly top in sugar beets. Based on this research, the Oregon Department of Agriculture received a Section 18 Crisis exemption for Poncho on January 27, 2006 which allows for the use of Poncho as a sugar beet seed treatment for the control of the virus vector in the following states: Colorado, Idaho, Montana, Oregon, Washington, and Wyoming. These efforts align with NP 303 Component IV - Pathogen Biology, Genetics, Population Dynamics, Spread, and Relationship with Hosts and Vectors. We have conducted research which established that disease resistance to curly top in sugar beets identified in an artificially inoculated nursery correlates with disease resistance and yield under commercial growing conditions. These data assure the sugar beet industry that disease resistance to curly top identified in the artificially inoculated nurseries will lead to enhanced disease resistance and yield. These efforts align with NP 303 Component IV - Pathogen Biology, Genetics, Population Dynamics, Spread, and Relationship with Hosts and Vectors. We have conducted preliminary studies to identify the primary organisms responsible for sugar beet root rot in the Intermountain West. These data will allow us to improve disease management options and guide plant breeding strategies for the control sugar beet root rot. These efforts align with NP 303 Component I - Identification and Classification of Pathogens. We have conducted two years of screening for resistance to rhizomania in Idaho. Our first year results showed that the strains of rhizomania in Idaho are capable of partially defeating the Rz1 gene, which is widely deployed commercially, and the Rz2 gene will increase effectiveness of control, but alone these genes are not enough. These data establish the urgency of finding new sources of resistance and the necessity of doing screening in Idaho fields with the new more severe strains of the virus. We have inoculated a 20 acre field in Kimberly, ID with a new strain of Beet necrotic yellow vein virus (BNYVV), the causal agent of rhizomania, to facilitate disease screening for novel sources of disease resistance. Germplasm screening conducted in this field will facilitate the identification of novel sources of disease resistance to BNYVV which is the primary means of controlling rhizomania. These efforts align with NP 301 Component II - Genome Characterization and Genetic Improvement; Problem Area IIb - Genetic Improvement. 6. What science and/or technologies have been transferred and to whom? When is the science and/or technology likely to become available to the end- user (industry, farmer, other scientists)? What are the constraints, if known, to the adoption and durability of the technology products? We have conducted research establishing Poncho as an effective insecticide seed treatment which will supplement host resistance for the control of curly top in sugar beets. Based on this research, the Oregon Department of Agriculture received a Section 18 Crisis exemption for Poncho on January 27, 2006, which allows for the use of Poncho as a sugar beet seed treatment in the following states: Colorado, Idaho, Montana, Oregon, Washington, and Wyoming. This technology has been transferred to the industry and growers through field tours, presentations, and publications. We have screened materials for ARS Fargo, ND, with SNP markers to facilitate their studies of powdery mildew resistance in sugar beets. We can now use these markers to assist other sugar beet researchers in the ARS with tagging genes of interest and for anchoring new markers to non- public maps developed in Europe. 7. List your most important publications in the popular press and presentations to organizations and articles written about your work. (NOTE: List your peer reviewed publications below). Dr. Gillen gave a presentation on the Kimberly Sugar Beet Program at the Plant and Animal Genome Conference in San Diego, CA (January 2006). Dr. Strausbaugh gave an invited talk on the programs curly top research work at the University of Idaho Sugar Beet School in Nampa, ID (January 2006). Dr. Strausbaugh gave an invited talk on the programs sugar beet storage research at the Amalgamated Sugar Company Seminar Series in Boise, ID (March 2006). Drs. Strausbaugh and Gillen participated in the sugar beet tour for Intermountain West production area (June 2006). Dr. Gillen participated in the Agriculture Twilight Tour giving out information on the Kimberly Sugar Beet program (July 2006). Dr. Strausbaugh gave an invited presentation on the sugar beet storage research to Syngentas European scientists (August 2006). Dr. Strausbaugh gave an invited presentation on the sugar beet storage research to the Western Sugar Cooperative (August 2006). Drs. Strausbaugh and Gillen presented research on the Amalgamated Sugar Company Variety Trial Tour (August 2006).

Impacts
(N/A)

Publications

  • Strausbaugh, C.A., Gallian, J.J., Camp, S., Foote, P., Gillen, A.M. 2005. Managing curly top. In: Proceedings of the University of Idaho 2005 Winter Commodity Schools. Snake River Sugarbeet Conference, January 14, 2005, Nampa, Idaho. 2005 CDROM.
  • Strausbaugh, C.A., Overturf, K.E., Koehn, A.C. 2005. Pathogenicity and real-time pcr detection of fusarium spp. in wheat and barley roots. Canadian Journal of Plant Pathology. 27:430-438.
  • Strausbaugh, C.A., Gillen, A.M., Shock, C.C., Eldredge, E.P. 2006. Evaluation of commercial sugar beet hybrids for resistance to beet curly top in Malheur County, Oregon, 2005. Biological and Cultural Tests for Control of Plant Diseases. 21:FC039.
  • Strausbaugh, C.A., Gillen, A.M., Shock, C.C., Eldredge, E.P. 2006. Evaluation of commercial sugar beet hybrids for resistance to beet curly top in Malheur County, Oregon, 2005. Biological and Cultural Tests for Control of Plant Diseases. 21:FC040.
  • Strausbaugh, C.A., Gillen, A.M., Gallian, J.J., Camp, S., Stander, J.R. 2006. Influence of host resistance and insecticide seed treatments on curly top in sugar beets [abstract]. Phytopathology. 96:S111.
  • Gillen, A.M., Strausbaugh, C.A. 2006. The new USDA-ARS sugarbeet germplasm development program at Kimberly, Idaho [abstract]. Plant and Animal Genome Abstracts. Paper No. W179.