Progress 10/01/09 to 09/30/10
Outputs
Progress Report Objectives (from AD-416) Develop inbred lines and hybrids adapted to the abiotic and biotic stresses characteristic of the Mid-Atlantic region and Corn Belt. Evaluate per se agronomic performance, and new breeding methods to most effectively utilize exotic germplasm. Approach (from AD-416) Seventy breeding crosses will be evaluated per se for adaptability and agronomic performance. Inbred line development will be initiated in five breeding crosses with the objective to self 500 plants and ultimately save 160 plants for advancing to S2 stage. The effectiveness of the single seed descent (SSD) method will be assessed by making top crosses of SSD progenies, and progenies derived from conventional GEM protocol. Final assessment will be made in 2009 yield trials. This project relates to the primary objectives of the parent project which includes the evaluation and development of germplasm for improved agronomic performance and resistance to abiotic and biotic stresses. During the course of this project, the University of Delaware evaluated more than 300 GEM breeding crosses for agronomic potential which included maturity, flowering synchrony, lodging resistance, stay green, disease, and insect resistance. These evaluations provided useful information for choosing exotic germplasm for future development. In 2009, 70 breeding crosses were evaluated and 17 were selected for future development. The GEM tropical breeding cross, DKXL212:N11a, has had good potential for inbred development resulting in the release of three inbreds (DE3, DE4, and DE6). Four breeding methods were evaluated and included the conventional GEM (CG), conventional mass selection (CM), modified single seed descent (MSSD), and doubled haploids (DH). Three GEM breeding crosses used in the methods study included ANTIG01:N16DE4, AR16035:S0209, and DKXL212:S0943b. The DH method was shown to have a wider range of values for yield, and grain moisture as expected based on greater additive variance among inbreds versus S2 lines. However, the CM and MSSD methods accounted for about one third of the top 10 lines based on yield, and yield/moisture ratio. It was concluded that the MSSD method utilizing S3 lines for testcrossing may be an effective alternative to DH, and the conventional methods for developing lines with improved yield. This project was terminated on 5/31/2010 since the collaborating PI left on a sabbatical assignment. No new project was initiated. The methods for monitoring activities include (1) observing germplasm developed by the Cooperator, and (2) progress reports submitted by the Cooperator in July and December for the Annual GEM Cooperator Meeting. E-mail exchanges and telephone discussions were periodically initiated by the ADODR or the Cooperator to discuss SSD experiments, and availability of new GEM germplasm resources which were provided by the ADODR. Top crosses and inbreds developed by the Cooperator have been planted and observed in the Ames nursery, amino acid experiments, and Field Day demonstration plot.
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Progress 06/07/06 to 05/31/10
Outputs
Progress Report Objectives (from AD-416) Develop inbred lines and hybrids adapted to the abiotic and biotic stresses characteristic of the Mid-Atlantic region and Corn Belt. Evaluate per se agronomic performance, and new breeding methods to most effectively utilize exotic germplasm. Approach (from AD-416) Seventy breeding crosses will be evaluated per se for adaptability and agronomic performance. Inbred line development will be initiated in five breeding crosses with the objective to self 500 plants and ultimately save 160 plants for advancing to S2 stage. The effectiveness of the single seed descent (SSD) method will be assessed by making top crosses of SSD progenies, and progenies derived from conventional GEM protocol. Final assessment will be made in 2009 yield trials. No progress in the current fiscal year. Agreement remained open for administrative closeout.
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Progress 10/01/08 to 09/30/09
Outputs
Progress Report Objectives (from AD-416) Develop inbred lines and hybrids adapted to the abiotic and biotic stresses characteristic of the Mid-Atlantic region and Corn Belt. Evaluate per se agronomic performance, and new breeding methods to most effectively utilize exotic germplasm. Approach (from AD-416) Seventy breeding crosses will be evaluated per se for adaptability and agronomic performance. Inbred line development will be initiated in five breeding crosses with the objective to self 500 plants and ultimately save 160 plants for advancing to S2 stage. The effectiveness of the single seed descent (SSD) method will be assessed by making top crosses of SSD progenies, and progenies derived from conventional GEM protocol. Final assessment will be made in 2009 yield trials. Significant Activities that Support Special Target Populations This project relates to the primary objectives of the parent project which includes the evaluation and development of germplasm for improved agronomic performance and resistance to abiotic and biotic stresses. Seventy new GEM breeding crosses are currently being evaluated for adaptability, maturity, flowering synchrony, standability, plant and ear height, pest resistance, stay green, grain quality and dry-down. Based on the 2008 breeding cross evaluations at the University of Delaware, the USDA-ARS GEM Project in Ames, IA, and two GEM private cooperators in Mount Vernon, IN, and Memphis, TN, 17 breeding crosses (out of 66 evaluated) were identified with high scores for per se performance. Based on per se observations, and European corn borer resistance 982 S1 selections were made with two stiff stalk populations (DKXL212:S0928 and DK212:S09), and three non-stiff stalk populations (BR105:N16a16b, CL00331:N1834, and CML329:N1834). The S2's are being made in summer 2009 by the modified single seed descent (SSD) breeding procedure. Fifty three lines derived from FSA(T):N1804 were selected from 2008 yield trials and are being advanced in the summer nursery. The methods for monitoring activities include (1) observing germplasm developed by the Cooperator, and (2) progress reports submitted by the Cooperator in July and December for the Annual GEM Cooperator Meeting. E-mail exchanges and telephone discussions were periodically initiated by the ADODR or the Cooperator to discuss SSD experiments, and availability of new GEM germplasm resources which were provided by the ADODR. Top crosses and inbreds developed by the Cooperator have been planted and observed in the Ames nursery, amino acid experiments, and Field Day demonstration plot.
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Progress 10/01/07 to 09/30/08
Outputs
Progress Report Objectives (from AD-416) Develop inbred lines and hybrids adapted to the abiotic and biotic stresses characteristic of the Mid-Atlantic region and Corn Belt. Evaluate per se agronomic performance, and new breeding methods to most effectively utilize exotic germplasm. Approach (from AD-416) Fifty breeding crosses will be evaluated per se for adaptability and agronomic performance. Inbred line development will be initiated in six breeding crosses with the objective to self 500 plants and ultimately save 160 plants for advancing to S2 stage. The effectiveness of the single seed descent (SSD) method will be assessed by making top crosses of SSD progenies, and progenies derived from conventional GEM protocol. Final assessment will be made in 2007 yield trials. Significant Activities that Support Special Target Populations This project relates to the primary objectives of the parent project which includes the evaluation and development of germplasm for improved agronomic performance and resistance to abiotic and biotic stresses. Sixty-six new GEM breeding crosses are currently being evaluated for adaptability, maturity, flowering synchrony, standability, plant and ear height, pest resistance, stay green, grain quality and dry-down. Based on the 2007 breeding cross evaluations at the University of Delaware, the USDA-ARS GEM Project in Ames, IA, and two GEM private cooperators in Mount Vernon, IN, and Memphis, TN, nine breeding crosses (out of 50 evaluated) were identified with high scores for per se performance. Six breeding cross populations (BR105:N16a16b, CL-00331:N1834, CML329:N1834, DKXL212:S0928, DK212T:S0640, and DKXL212:S09) were selected to initiate selfing in 2008. Based on per se evaluations for plant height, ear placement, stalk and root strength, ear traits, maturity, disease and European corn borer (ECB) resistance, 642 S1 selections were made from four stiff stalk breeding crosses (DKXL212:S09, AR16021:S099j, AR16021:S0908c, and AR16021:S08b09b). Two hundred eighteen S2 ears were selected from six breeding crosses that had been advanced from the S1 stage by a modified single seed descent (MSSD) bulk procedure. One hundred two lines from the MSSD(S2) per se open-pollinated ears observation nursery were selected for further evaluation of yield and other agronomic traits. In summer 2008, 370 S2/S3 lines are being advanced. Yield test evaluation of 134 hybrids crossed to HC33 tester is being conducted at Delaware, and USDA-ARS GEM location in Ames, IA. We are collaborating with the Southern GEM Project to evaluate 535 hybrids in Raleigh, NC. The methods for monitoring activities include (1) observing germplasm developed by the Cooperator, and (2) progress reports submitted by the Cooperator in July and December for the Annual GEM Cooperator Meeting. E-mail exchanges and telephone discussions were periodically initiated by the ADODR or the Cooperator to discuss SSD experiments, and availability of new GEM germplasm resources which were provided by the ADODR. Top crosses and inbreds developed by the Cooperator have been planted and observed in the Ames nursery, amino acid experiments, and Field Day demonstration plot.
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Progress 10/01/06 to 09/30/07
Outputs
Progress Report Objectives (from AD-416) Develop inbred lines and hybrids adapted to the abiotic and biotic stresses characteristic of the Mid-Atlantic region and Corn Belt. Evaluate per se agronomic performance, and new breeding methods to most effectively utilize exotic germplasm. Approach (from AD-416) Fifty breeding crosses will be evaluated per se for adaptability and agronomic performance. Inbred line development will be initiated in six breeding crosses with the objective to self 500 plants and ultimately save 160 plants for advancing to S2 stage. The effectiveness of the single seed descent (SSD) method will be assessed by making top crosses of SSD progenies, and progenies derived from conventional GEM protocol. Final assessment will be made in 2007 yield trials. Significant Activities that Support Special Target Populations This report serves to document research conducted under a specific cooperative agreement between ARS and the University of Delaware (UD). Additional details of research can be found in the report for the parent project 3625-21000-047-00D, "Germplasm Enhancement of Maize Project (GEM) ". This project relates to the primary objectives of the parent project which includes the evaluation and development of germplasm for improved agronomic performance and resistance to abiotic and biotic stresses. Fifty new GEM breeding crosses are currently being evaluated for adaptability, maturity, flowering synchrony, standability, plant and ear height, pest resistance, stay green, grain quality and dry-down. Based on the 2006 breeding cross evaluations at the University of Delaware, the USDA-ARS GEM Project in Ames, Iowa, and two GEM private cooperators in Mount Vernon, Indiana, and Memphis, TN four breeding populations were selected to initiate selfing in 2007. In summer 2007, S1 lines derived from six GEM breeding crosses are being advanced to S2 using the modified single seed descent method (SSD), 355 S2 lines are being observed under open pollination, and 85 S2 and S3 lines are being advanced to S3 or S4 respectively. Approximately 530 top cross hybrids originating from the Southern GEM Project in Raleigh are being evaluated in Delaware. First year yield trials of lines derived from the SSD method are being evaluated in locations managed by the USDA-ARS GEM Project in Ames in 2007. The methods for monitoring activities include (1) observing germplasm developed by the Cooperator, and (2) progress reports submitted by the Cooperator in July and December for the Annual GEM Cooperator Meeting. E-mail exchanges and telephone discussions were periodically initiated by the ADODR or the Cooperator to discuss SSD experiments, and availability of new GEM germplasm resources which were provided by the ADODR. Top crosses and inbreds developed by the Cooperator have been planted and observed in the Ames nursery, amino acid experiments, and Field Day demonstration plot. A site visit to Ames will be made by the Cooperator in September.
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Progress 10/01/05 to 09/30/06
Outputs
Progress Report 4d Progress report. This report serves to document research conducted under a specific cooperative agreement between ARS and the University of Delaware (UD). Additional details of research can be found in the report for the parent CRIS 3625-21000-047-00D, "Germplasm Enhancement of Maize Project (GEM)". This project relates to the primary objectives of the parent project which includes the evaluation and development of germplasm for improved agronomic performance and resistance to abiotic and biotic stresses. Fifty new GEM breeding crosses are being evaluated for adaptability, maturity, and numerous agronomic and disease traits. Our previous results from the year 2005 identified six promising breeding crosses based on observations in Delaware, southern Indiana, and Ames, IA. These six populations are being worked in the nursery for the purpose of generating new S1 families for further development in 2007. In summer, 2006, S1 lines from four GEM
breeding crosses are being advanced. The collaborative experiment with GEM in Ames, IA is continuing with the generation of top cross seed in Ames for 165 S2 lines derived from FS8A(T) :N1804. Yield trials in 2006 include 503 top crosses at three irrigated locations in Delaware, and one location in Ames, IA. Most of the yield trials are in good condition, although the Smyrna and Magnolia, DE locations have above average root lodging and green snap as a result of the Eastern storms in late June. A Crop Science germplasm release was published in late 2005 for the GEM derived lines, DE3 and DE4. An additional manuscript is under preparation for DE6, a high protein line. 7. List your most important publications in the popular press and presentations to organizations and articles written about your work. (NOTE: List your peer reviewed publications below). Hawk, J.A., and Weldekidan, T. 2005. Registration of DE3, DE4, and DE5 Parental Lines of Maize. Crop Sci. 45:2669-2670.
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