Progress 10/01/09 to 09/30/10
Outputs
Progress Report Objectives (from AD-416) Develop a genomic framework by integrating physical map data with existing genetic map, and ECO-TILLING of new alleles for genes related to resistance, industrial, and nutraceutical traits. Approach (from AD-416) Development of a genetic population segregating for pest resistance, horticultural, and nutraceutical traits. Construction of a BIBAC library, and making BAC pools and physical mapping for a multiple pest-resistant ARS cultivar. Genetic population will be characterized for the resistance and other traits. ECOTILLING will be used to identify genetic marker variants that may be correlated with these traits. This research relates to inhouse objective objective 2, �Evaluate new sources of resistance against insect pests of sweetpotato and facilitate the incorporation of known resistance sources into advanced sweetpotato breeding lines.� The ADODR monitored activities of project through telephone calls and emails. Studies continued on a segregating genetic population of the cross �Excel� x SC1149-19. This population of 160 clones was grown in replicated plots in 2009 at the U.S. Vegetable Laboratory (USVL), Charleston, SC, and evaluated for pest resistance, yield, color, and leaf shape. Results were sent to the principal investigators.
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Progress 10/01/08 to 09/30/09
Outputs
Progress Report Objectives (from AD-416) Develop a genomic framework by integrating physical map data with existing genetic map, and ECO-TILLING of new alleles for genes related to resistance, industrial, and nutraceutical traits. Approach (from AD-416) Development of a genetic population segregating for pest resistance, horticultural, and nutraceutical traits. Construction of a BIBAC library, and making BAC pools and physical mapping for a multiple pest-resistant ARS cultivar. Genetic population will be characterized for the resistance and other traits. ECOTILLING will be used to identify genetic marker variants that may be correlated with these traits. Significant Activities that Support Special Target Populations This project relates to in-house Objective 3: Develop cost effective, biologically-based technologies for use in integrated pest management (IPM) programs for sweetpotato, cucurbits, and other vegetable crops. Studies were continued using a segregating genetic population of the cross �Excel� x SC1149-19. This population of 160 clones was grown in replicated plots in 2008 and 2009 at the US Vegetable Laboratory (USVL), Charleston, SC, and evaluated for pest resistance, yield, color, and leaf shape. Results were sent to the principal investigator who is constructing a Binary Bacterial Artificial Chromosome (BIBAC) library and developing a physical map of the pest-resistant cultivar Excel. A second set of 100 sweetpotato clones that were grown in replicated 12-plant plots at the USVL in 2008 were evaluated for yield, quality factors (shape, color, texture, taste, etc.), and resistance to soil insect pests. These data were also sent to the principal investigator. Activities of project were monitored through telephone calls and emails.
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Progress 10/01/07 to 09/30/08
Outputs
Progress Report Objectives (from AD-416) Develop a genomic framework by integrating physical map data with existing genetic map, and ECO-TILLING of new alleles for genes related to resistance, industrial, and nutraceutical traits. Approach (from AD-416) Development of a genetic population segregating for pest resistance, horticultural, and nutraceutical traits. Construction of a BIBAC library, and making BAC pools and physical mapping for a multiple pest-resistant ARS cultivar. Genetic population will be characterized for the resistance and other traits. ECOTILLING will be used to identify genetic marker variants that may be correlated with these traits. Significant Activities that Support Special Target Populations This project is related to Objective 2 of this in-house project: Evaluate new sources of resistance against insect pests of sweetpotato and facilitate the incorporation of known resistance sources into advanced sweetpotato breeding lines. Activities of this project were monitorted through telephone calls and email. Studies continued on a segregating genetic population of the cross of �Excel� and SC1149-19. This population of 160 clones was grown in replicated plots at the US Vegetable Laboratory (USVL), Charleston, SC. These genotypes were evaluated for pest resistance, yield, color, and leaf shape. Results of field evaluations were sent to the principal investigator who is constructing a BIBAC library and physical mapping of the pest-resistant cultivar, Excel. A second set of 100 sweetpotato genotypes were received in tissue culture tubes from a cooperating scientist. These genotypes were multiplied through greenhouse cuttings and planted in replicated 12-plant plots at the USVL. At harvest, these genotypes will be evaluated for insect and disease resistance, color, shape, and yield, and data will be sent to the principal investigator. Tissue culture tubes of several clones were sent to a cooperator at Alabama A&M University.
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Progress 10/01/06 to 09/30/07
Outputs
Progress Report Objectives (from AD-416) Develop a genomic framework by integrating physical map data with existing genetic map, and ECO-TILLING of new alleles for genes related to resistance, industrial, and nutraceutical traits. Approach (from AD-416) Development of a genetic population segregating for pest resistance, horticultural, and nutraceutical traits. Construction of a BIBAC library, and making BAC pools and physical mapping for a multiple pest-resistant ARS cultivar. Genetic population will be characterized for the resistance and other traits. ECOTILLING will be used to identify genetic marker variants that may be correlated with these traits. Significant Activities that Support Special Target Populations This report documents research conducted under a reimbursable agreement between ARS and West Virginia State College. Additional details of the research can be found in the report for the in-house associated project 6659-22000-18-00D, New Approaches for Insect Pest Management in Vegetable Crops. A genetic population segregating for pest resistance was established by crossing two sweetpotato clones, Excel and SC1149-19. Excel is highly resistant to multiple species of insect pests; SC1149-19 is susceptible. The resulting population of 160 clones was grown in replicated plots at the U.S. Vegetable Laboratory in Charleston, SC. These 160 clones were evaluated for pest resistance, yield, root color, and leaf shape. Tissue culture tubes of all 160 clones were sent to the principal investigator at Alabama A&M University. The principal investigator at Alabama A&M University has initiated construction of a BIBAC library and physical mapping of the pest-resistant cultivar. Coordination and project monitoring was done through conference calls and e-mail messages.
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Progress 10/01/05 to 09/30/06
Outputs
Progress Report 4d Progress report. This report serves to document research conducted under a reimbursable trust agreement between ARS and West Virginia State University. Additional details of research can be found in the parent CRIS 6659-21000- 015-00D Genetic Improvement of Sweetpotato and Snap Bean for Multiple Pest Resistance and New Uses. There has been relatively little work on mapping the sweetpotato genome, despite the fact that sweetpotato is the seventh largest food crop in the world. Attempts to map the sweetpotato using conventional markers like RAPDs and AFLPs have shown that the sweetpotato genome is a very complex polyploid. The purpose of this project is to use emerging technology for association mapping and linkage disequilibrium analysis in sweetpotato. The technique to be applied to sweetpotato mapping is known as Targeting Induced Local Lesions in Genomes, (TILLING), or in seedling populations - ECOTILLING. This approach will be used to mine for
new alleles related to pest resistance, food quality, processing potential, and nutraceutical compounds. This was a new project in FY2006. To date, the principal investigator from WV State University has constructed a BAC library. The ARS Research Geneticist has developed the seedling mapping population. These seedlings were cloned and placed in tissue culture to ship to the second subcontract investigator at Alabama A& M University. The ARS research scientist on this project will evaluate the genetic traits in this seedling population to be used in map construction. These traits include resistance to insects, morphological traits, and other horticultural traits.
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