Progress 09/01/05 to 08/31/09
Outputs
Progress Report Objectives (from AD-416) The purpose of this agreement is to characterize the biological response to dietary exposure to naturally occurring dietary constituents, allyl isothiocyanates (AITC). The proposed studies will compare the availability and metabolism of AITC from various food sources and contrast the response in terms of ability to reduce DNA damage and to monitor genetic and proteomic expression changes resulting from dietary exposure. Approach (from AD-416) Human studies will be conducted with subjects consuming naturally occurring constituents found in cruciferous vegetables, namely allyl isothiocyanates. Healthy adults will be assigned to treatments consisting of varying amounts and/or types of allyl isothiocyanate-rich foods (cruciferous vegetables such as Brussels sprouts, cabbage, Wasabi) or commercially available extracts containing allyl isothiocyanates. Intake levels will not be pharmacological, but rather will be limited to levels achievable with commonly consumed amounts of allyl isothiocyanate- containing foods. Subjects will consume the foods for up to 60 days, and biological samples will be collected and analyzed. Availability of parent compounds and their metabolites will be determined. Target outcomes include indices of oxidation and changes in genetic and proteomic expression. Significant Activities that Support Special Target Populations Brassica vegetables, such as broccoli, Brussels sprouts, cabbage, and mustard, provide dietary allyl isothiocyanate, a compound which is thought to have cancer preventative properties. In collaboration with the NCI, through this agreement the laboratory has conducted a study of the absorption of allyl isothiocyanate and its potential to decrease cancer risk factors. Previously, a clinical study was conducted in which volunteers consumed a base diet with additions of cabbage and mustard or mustard oil, and blood and urine samples were collected from volunteers. Analysis of those samples indicated that daily ingestion of cabbage and mustard, as well as mustard oil, influenced DNA integrity. During 2009 follow-up studies with isolated cells were conducted to confirm that finding, and planned additional studies to identify specific metabolic pathways in cells that are influenced by a key nutrient found in cabbage and mustard. These results will benefit health professionals in understanding healthful diets. Activities are monitored through E-mail, periodic phone calls, and site visits.
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Progress 10/01/06 to 09/30/07
Outputs
Progress Report Objectives (from AD-416) The purpose of this agreement is to characterize the biological response to dietary exposure to naturally occurring dietary constituents, allyl isothiocyanates (AITC). The proposed studies will compare the availability and metabolism of AITC from various food sources and contrast the response in terms of ability to reduce DNA damage and to monitor genetic and proteomic expression changes resulting from dietary exposure. Approach (from AD-416) Human studies will be conducted with subjects consuming naturally occurring constituents found in cruciferous vegetables, namely allyl isothiocyanates. Healthy adults will be assigned to treatments consisting of varying amounts and/or types of allyl isothiocyanate-rich foods (cruciferous vegetables such as Brussels sprouts, cabbage, Wasabi) or commercially available extracts containing allyl isothiocyanates. Intake levels will not be pharmacological, but rather will be limited to levels achievable with commonly consumed amounts of allyl isothiocyanate- containing foods. Subjects will consume the foods for up to 60 days, and biological samples will be collected and analyzed. Availability of parent compounds and their metabolites will be determined. Target outcomes include indices of oxidation and changes in genetic and proteomic expression. Significant Activities that Support Special Target Populations This report documents research conducted under a reimbursable agreement between ARS and the National Cancer Institute (NCI). Additional details of research can be found in the report for the in-house associated project, 1235-51000-046-00D, "Bioavailability and Metabolism of Phytochemicals and Other Micronutrients." Brassica vegetables, such as broccoli, brussels sprouts, cabbage, and mustard, provide dietary allyl isothiocyanate, a compound which is thought to have great cancer preventative properties. In collaboration with the NCI, the laboratory is conducting a study of the absorption of allyl isothiocyanate and its potential to decrease cancer risk factors. During 2007, a protocol to conduct the study was submitted to an Institutional Review Board (IRB) and approved. In the study, volunteers consumed a base diet with additions of cabbage and mustard or mustard oil, and blood and urine samples were collected from the volunteers for analysis of cancer risk factors. Sample analysis is in progress. These results will benefit consumers and health professionals as diets are planned. The activities are monitored through in-person involvement in research studies and through team research meetings; and reports progress to funding agency personnel at group meetings and during conference calls.
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Progress 10/01/05 to 09/30/06
Outputs
Progress Report 4d Progress report. This report serves to document research conducted under a Reimbursable Agreement with the National Cancer Institute (NCI). Additional details of the research can be found in the report for the parent project 1235-51000- 046-00D, Bioavailability and Metabolism of Phytochemicals and Other Micronutrients. Brassica vegetables, such as broccoli, Brussels sprouts, cabbage, and mustard, provide dieatary allyl isothiocyanate, a compound that is thought to have great cancer preventative properties. In collaboration with the NCI, the Beltsville Human Nutrition Research Centers Diet and Human Performance Lab has begun a study of the absorption of allyl isothiocyanate and its potential to decrease cancer risk factors. A thorough literature search has been completed, and the clinical study has been designed. The Institutional Review Board protocol has been written and approved. The results of the study will benefit scientists studying cancer
prevention and consumers and health professionals in planning healthful diets.
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