Progress 04/01/05 to 03/31/08
Outputs
Progress Report Objectives (from AD-416) Map QTL conditioning high protein content in soybean seeds to validate previously identified protein QTL and use them to screen for protein in a new population. Approach (from AD-416) Compare QTLs associated with high seed protein content in two types of populations: those derived from crosses between high-yield average- protein lines and lines that have high-protein, and those derived from crosses between lines with high-yield, above average protein content and high-protein lines. Validate QTLs for high protein content reported in the population derived from Young x PI416937 by mapping QTLs in a related population derived from Brim x PI416937. Significant Activities that Support Special Target Populations This project is related to Objective 2 of this in-house project: To characterize the biological mechanisms that control phytic acid concentration in soybean seed, and determine the impact of genetically lowering phytic acid on seed and seedling vigor, soy protein functionality and soybean meal digestibility. The population, Brim x PI 416,937, was genotyped at simple sequence repeat (SSR) loci near previously reported quantitative trait gene loci (QTL). One high protein QTL was validated in which the positive allele is inherited from PI416937 on LG C1. Oil is decreased but only significantly. This protein QTL had no significant effects on flowering date, maturity, lodging, yield, and height. The F6-derived Prolina x Brim population was genotyped at SSR loci near previously reported QTL. There was no association between protein content and those SSR markers. We could not validate those previously reported QTL because the population sample size was not large enough. Significant genetic variation for protein content was found among lines from the Prolina x PI416937 population. There was significant transgressive segregation, and lines with protein content higher than Prolina were observed. These results demonstrate that Prolina and PI416937 have at least some genes that affect protein content that are different. Marker assisted selection was performed on lines and plants from the PI416937 x NC-Roy population. Lines with SSRs Sat_367, Sat_140, Satt396 were genotyped, and lines that inherited the alleles from PI416937 were selected. In 2006, the mean protein content of F4-derived lines that inherited the PI416937 alleles at Sat_367, Sat_140, Satt396, and Satt578 was significantly higher than the protein content of NC Roy. In 2006, the mean protein content of F2-derived lines that inherited the PI416937 alleles at Sat_367, Sat_140, Satt396, and Satt578 was significantly higher than the protein content of NC Roy and significantly lower than PI416937. Both results demonstrate that MAS selection worked and confirmed the existence of the high protein QTL on LG C1. Some lines had a protein content equal to NC-Roy, demonstrating that protein content is regulated by many genes, that the selection only targeted one of them, and that the proportion of selected lines that have high protein content was increased. The Authorized Departmental Officer's Designated Representativemonitored activities of project through frequent phone calls and emails, two formal meetings, site visits, and through quarterly reports.
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Progress 10/01/06 to 09/30/07
Outputs
Progress Report Objectives (from AD-416) Map QTL conditioning high protein content in soybean seeds to validate previously identified protein QTL and use them to screen for protein in a new population. Approach (from AD-416) Compare QTLs associated with high seed protein content in two types of populations: those derived from crosses between high-yield average- protein lines and lines that have high-protein, and those derived from crosses between lines with high-yield, above average protein content and high-protein lines. Validate QTLs for high protein content reported in the population derived from Young x PI416937 by mapping QTLs in a related population derived from Brim x PI416937. Significant Activities that Support Special Target Populations This report serves to document research conducted under a specific cooperative agreement between ARS and the Agricultural Research Station, North Carolina State University. Additional details of research can be found in the report for the parent project 6645-21000-026-00D, Physiological/biochemical mechanisms associated with genetic alteration of soybean quality and productivity. Marker assisted selection was performed on inbred lines and F2 plants from a population derived from crossing PI416937 with NC-Roy. We genotyped lines with three SSR markers, Sat_367, Sat_140, and Satt396. Lines that inherited the alleles from PI416937 were selected. Those lines were tested in yield trials at two NC locations. The F2 plants were tested in an unreplicated augmented design at one location. 80 F4 derived lines from a cross of Prolina with PI416937 were tested at one field location to determine if there is segregation distortion for protein content in this population. If segregation distortion is observed then it means that these two lines carry different genes for high protein content and thus can be combined in a breeding program. Improved meal quality provided by these soybeans will increase the competitiveness of U.S. soybean producers in the global market and provide a better protein meal for domestic users. The ADODR monitored activities through quarterly progress reports, 1 meeting with researchers, emails and phone calls.
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Progress 10/01/05 to 09/30/06
Outputs
Progress Report 4d Progress report. This report serves to document research conducted under a specific cooperative agreement between ARS and the Agricultural Research Station, North Carolina State University. Additional details of research can be found in the report for the parent project 6645-21000-025-00D, Fundamental mechanisms for genetic alteration of soybean quality and productivity. One region on chromosome C in the soybean genome was validated as carrying high protein gene/s that are inherited from the germplasm, PI416937. This high protein gene is not expected to be in other soybean varieties because PI416937 has not been used extensively in US breeding programs. In addition, this region seems to reduce the oil content but has no significant effects on flowering date, maturity, lodging, yield, and height. This gene can be introduced into productive soybean varieties to increase the protein content. Improved meal quality provided by these soybeans will
increase the competitiveness of U.S. soybean producers in the global market and provide a better protein meal for domestic users.
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Progress 10/01/04 to 09/30/05
Outputs
4d Progress report. This report serves to document research conducted under a specific cooperative agreement between ARS and the Agricultural Research Station, North Carolina State University. Additional details of research can be found in the report for the parent project 6645-21000-025-00D, Fundamental mechanisms for genetic alteration of soybean quality and productivity. This project was initiated on April 1, 2005. It is too early to report any significant progress at this time.
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