Source: GATORADE SPORTS SCIENCE INSTITUTE submitted to NRP
ASSESSMENT OF MINERAL LOSSES IN SWEAT DURING PHYSICAL ACTIVITY
Sponsoring Institution
Agricultural Research Service/USDA
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
0409328
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
May 26, 2005
Project End Date
Dec 31, 2009
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
GATORADE SPORTS SCIENCE INSTITUTE
617 WEST MAIN STREET
BARRINGTON,IL 60010
Performing Department
(N/A)
Non Technical Summary
(N/A)
Animal Health Component
30%
Research Effort Categories
Basic
60%
Applied
30%
Developmental
10%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
7023840101020%
7026010101060%
7026020101010%
7026099101010%
Goals / Objectives
This project will evaluate the feasibility of determining sweat losses of mineral elements in humans during periods of controlled physical activity. This study is an initial effort to develop a valid and reliable method to determine surface mineral losses. This method is needed to improve assessment of mineral nutritional needs of physically active people.
Project Methods
The cooperator will recruit and enroll skilled athletes to participate in the approved study to determine the effects of physical activity on body sweat losses. The cooperator will provide unique sweat collection apparatus and position them on various sites of the body before controlled physical activities, monitor mineral-containing beverage intake, then remove the sweat collection devices. Sweat will be extracted and sent to the USDA, ARS Grand Forks Human Nutrition Research Center for determination of mineral concentrations. Data will be shared between ARS and cooperator scientists and prepared for publication in a scientific journal.

Progress 05/26/05 to 12/31/09

Outputs
Progress Report Objectives (from AD-416) This project will evaluate the feasibility of determining sweat losses of mineral elements in humans during periods of controlled physical activity. This study is an initial effort to develop a valid and reliable method to determine surface mineral losses. This method is needed to improve assessment of mineral nutritional needs of physically active people. Approach (from AD-416) The cooperator will recruit and enroll skilled athletes to participate in the approved study to determine the effects of physical activity on body sweat losses. The cooperator will provide unique sweat collection apparatus and position them on various sites of the body before controlled physical activities, monitor mineral-containing beverage intake, then remove the sweat collection devices. Sweat will be extracted and sent to the USDA, ARS Grand Forks Human Nutrition Research Center for determination of mineral concentrations. Data will be shared between ARS and cooperator scientists and prepared for publication in a scientific journal. Significant Activities that Support Special Target Populations The project constituted collaborative studies of the Grand Forks Human Nutrition Research Center and the Gatorade Sport Science Center to determine the composition of mineral nutrients in sweat. We completed the analyses of sweat and interstitial fluid samples collected from exercising volunteers, finding that sweat contains significant amounts of zinc and calcium. We found that supplemental zinc reduced the incidence of diarrhea in Kenyan adults. ADODR monitoring included phone calls and e-mails.

Impacts
(N/A)

Publications


    Progress 10/01/06 to 09/30/07

    Outputs
    Progress Report Objectives (from AD-416) This project will evaluate the feasibility of determining sweat losses of mineral elements in humans during periods of controlled physical activity. This study is an initial effort to develop a valid and reliable method to determine surface mineral losses. This method is needed to improve assessment of mineral nutritional needs of physically active people. Approach (from AD-416) The cooperator will recruit and enroll skilled athletes to participate in the approved study to determine the effects of physical activity on body sweat losses. The cooperator will provide unique sweat collection apparatus and position them on various sites of the body before controlled physical activities, monitor mineral-containing beverage intake, then remove the sweat collection devices. Sweat will be extracted and sent to the USDA, ARS Grand Forks Human Nutrition Research Center for determination of mineral concentrations. Data will be shared between ARS and cooperator scientists and prepared for publication in a scientific journal. Significant Activities that Support Special Target Populations This report serves to document research conducted under non-funded cooperative agreement between ARS and Gatorade Sport Science Institute. Additional information can be found in the report for the parent project 5450-51530-009-00D Micronutrient Roles in Physiology and Health. Progress includes the development of an appropriate reference material, pooled sweat reference, for use in quality control assessment of the analytical method to determine mineral concentrations in human sweat. The mineral composition of sweat collected during physical activity among youth is unknown. Sweat was collected during practice from male and female elite youth soccer players in different age groups (9-10 y, 11-12 y, and 14-15 y) to ascertain if mineral concentrations were different by gender and compared to data from adults. Sodium concentrations tended to increase with age, and males had greater concentrations of sodium in sweat than females. Magnesium concentrations in sweat of these youth were less than the lowest concentrations reported in adults also exercising. Activities were monitored by using monthly conference calls. Collaborators jointly organized a symposium at the American College of Sports Medicine and presented findings of this research.

    Impacts
    (N/A)

    Publications


      Progress 10/01/05 to 09/30/06

      Outputs
      Progress Report 4a List the single most significant research accomplishment during FY 2006. Sweat Mineral Losses in Youth During Controlled Exercise We performed the first study to evaluate mineral concentrations from specific sites on the bodies of youth and adolescent athletes during physical activity. The principal finding was that sweat mineral concentrations differed by sex; mineral concentrations were greater in males than females. Collaborators from Gatorade Sports Science Institute (Barrington, IL) recruit members of elite youth soccer teams to wear unobtrusive sweat collection patches during training sessions. This preliminary information is important in planning future studies to assess the impact of surface mineral losses on mineral nutritional status of humans. This work is related to Human Nutrition Action Plan 107 and addresses the area of Nutrient Requirements. 4d Progress report. This report serves to document research conducted under non-funded cooperative agreement #58-5450-5-107N between ARS and Gatorade Sport Science Institute. Additional information can be found in the report for the parent project 5450-51530-009-00D Micronutrient Roles in Physiology and Health. Another study is underway to develop a standard reference material for use in analysis of human sweat samples. The only available reference material with a similar matrix as sweat is an NIST water standard. However, the mineral concentrations are not in the same ranges as those found in human sweat. Thus, there is a critical need to develop this standard for future research.

      Impacts
      (N/A)

      Publications


        Progress 10/01/04 to 09/30/05

        Outputs
        4d Progress report. This report serves to document research conducted under non-funded cooperative agreement #58-5450-5-107N between ARS and Gatorade Sport Science Institute. Additional information can be found in the report for the parent project 5450-51530-009-00D Micronutrient roles in physiology and health. A study to determine the effects of physical training in elite male and female athletes was planned and is in progress. Sweat samples will be collected from various regions of the body during intense physical activity. The samples will be prepared by the research collaborators and delivered to the Center for analyses of mineral element concentrations.

        Impacts
        (N/A)

        Publications