Progress 09/15/04 to 09/14/08
Outputs
Progress Report Objectives (from AD-416) Determine genome organization of Boophilus microplus. Approach (from AD-416) Determine chromosome identity in Boophilus microplus and meiotic nuclei, and physically position genomic and cDNA sequences on chromosomes. Significant Activities that Support Special Target Populations The major objective of this project is to determine genome organization of Boophilus microplus. This information will be critical to assembling genome sequence information derived from a tick genome sequencing project. This project was originally due to expire in 2006 but was extended to allow the preparation of a manuscript describing the research. This manuscript was delayed due to an unexpected death in the Purdue cooperator's laboratory and has been submitted to the journal Chromosome Research. Under the scope of the agreement, 1,453 BAC end sequences have been obtained and a search for microsatellite markers and repeats that might be useful in chromosomal studies is underway. Additionally, two BAC clones with sequence homology to OP-resistance associated genes have been identified through filter hybridization protocols and have been sequenced to completion. A NRI grant proposal has been submitted in June 2008 to seek extramural funding to expand these studies. The ADODR monitors project activities by phone and conference calls, site visits, and meetings.
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Progress 10/01/06 to 09/30/07
Outputs
Progress Report Objectives (from AD-416) Determine genome organization of Boophilus microplus. Approach (from AD-416) Determine chromosome identity in Boophilus microplus and meiotic nuclei, and physically position genomic and cDNA sequences on chromosomes. Significant Activities that Support Special Target Populations This report documents research conducted under a specific cooperative agreement between ARS and Purdue University. Additional details of research can be found in the report for the in-house associated project CRIS 6205-32000-026-00D, Molecular Biology of Boophilus microplus. The major objective of this project is to determine genome organization of R. microplus. This information will be critical to assembling genome sequence information derived from a tick genome sequencing project. This project was originally due to expire in 2006 but was extended to allow the preparation of a manuscript describing the research. This manuscript was delayed due to an unexpected death in the Purdue cooperator's laboratory and is in the drafting process at Purdue University and expected to be submitted in 2007. A NRI grant proposal has been submitted in June 2007 to seek extramural funding to expand these studies. The ADODR monitors project activities by phone and conference calls, site visits, and meetings. This is a final report.
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Progress 10/01/05 to 09/30/06
Outputs
Progress Report 4d Progress report. This report serves to document research conducted under a specific cooperative agreement between ARS and Purdue University. Additional details of research can be found in the report for the parent CRIS 6205- 32000-026-00D, Molecular Biology of Boophilus microplus. The major objective of this project is to determine genome organization of B. microplus. This information will be critical to assembling genome sequence information derived from a tick genome sequencing project. Genomic clones have been localized to specific tick chromosomes to be utilized as chromosome markers. The pyrethroid-metabolizing esterase, CzEST9, has been used to localize the gene coding regions for this protein to a specific chromosome. A NRI grant proposal has been submitted to seek extramural funding to expand these studies and support a postdoc position at Kerrville.
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Progress 10/01/04 to 09/30/05
Outputs
4d Progress report. This report serves to document research conducted under a specific cooperative agreement between ARS and Purdue University. Additional details of research can be found in the report for the parent project, 6205-32000-026-00D, Molecular Biology of Boophilus microplus. The major objective of this project is to determine genome organization of B. microplus. This information will be critical to assembling genome sequence information derived from a tick genome sequencing project. Techniques to allow the identification of individual chromosomes of B. microplus have been developed utilizing dissected tick testes, synganglia, and accessory gland and highly purified tick genomic DNA. Fluorescence microscopy techniques have resulted in the identification of DNA clones that can serve as markers for specific chromosomes. Work is underway to identify the chromosomal number and location of B. microplus genes that have roles in pesticide resistance.
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