DETERMINATION OF AMINO ACID COMPOSITION IN SOYBEAN AND SOYBEAN PRODUCTS

• Sponsoring Institution: Agricultural Research Service/USDA
• Project Status: COMPLETE
• Funding Source: USDA INHOUSE
• Reporting Frequency: Annual
• Accession No.: 0408908
• Project Start Date: Oct 1, 2004
• Project End Date: Dec 31, 2005
• Program Code: [(N/A)]- (N/A)

Recipient Organization
AGRICULTURAL RESEARCH SERVICE
(N/A)
RALEIGH,NC 27695

Performing Department
(N/A)

Non Technical Summary
(N/A)

Animal Health Component

0%

Research Effort Categories

Basic

100%

Applied

0%

Developmental

0%

Classification

Knowledge Area (KA)	Subject of Investigation (SOI)	Field of Science (FOS)	Percent
201	1820	1040	30%
204	1820	1080	60%
302	1820	1010	10%

Knowledge Area
302 - Nutrient Utilization in Animals; 204 - Plant Product Quality and Utility (Preharvest); 201 - Plant Genome, Genetics, and Genetic Mechanisms;

Subject Of Investigation
1820 - Soybean;

Field Of Science
1040 - Molecular biology; 1010 - Nutrition and metabolism; 1080 - Genetics;

Keywords

quantitative

analysis

protein

isolates

phytate

phosphorus

qtl

Goals / Objectives
To develop an analytical method that accurately and reliably measures amino acid content in soybean, its meals and protein isolates.

Project Methods
Will perform the gas-phase protein hydrolysis of soybean samples including full-fat meal, defatted meal, insoluble and soluble proteins so that the protein hydrolysis procedures can be established and standardized; perform amino acid determination in the protein hydrolysates obtained from acid digestion with an HPLC system using pre-column derivatization technique for the detection of amino acids; determine the stability of the derivatized samples and make improvement so that automated HPLC system can be developed for large numbers of samples.

Progress 10/01/04 to 12/31/05

Outputs
Progress Report 4d Progress report. This report serves to document research conducted under the project 6645- 21000-025-63R, Determination of amino acid composition in soybean and soybean products. Additional details of research can be found in the report for the parent project 6645-21000-025-00D, Fundamental mechanisms for genetic alteration of soybean quality and productivity. Amino acid composition of soy protein is determined by protein hydrolysis and analyses of its hydrolysate with precision chromatography. Procedure for gas-phase hydrolysis of sample has been successfully established. The amino acid analysis procedure using precolumn derivatization was simple and the chromatography was very accurate. Reliability of the newly developed procedures for both hydrolysis and amino acid analysis were tested and compared with reference checks from other laboratories using a set of 25 soybean lines that were replicated twice in two different field locations. Statistical error rate was very low. Only two amino acids showed significant interactions with environment and five (glycine, valine, arginine, histine, and alanine) showed apparent genetic differences among the 24 lines. This more rapid method for measuring amino acids, will be an aid to soybean breeders in improving the amino acid composition of soy protein.

Impacts
(N/A)

Publications

Progress 10/01/04 to 09/30/05

Outputs
4d Progress report. This report serves to document research conducted under the project 6645- 21000-025-63R, Determination of amino acid composition in soybean and soybean products. Additional details of research can be found in the report for the parent project 6645-21000-025-00D, Fundamental mechanisms for genetic alteration of soybean quality and productivity. Amino acid composition of soy protein is determined by protein hydrolysis and analyses of its hydrolysate with precision chromatography. Procedure for gas-phase hydrolysis of sample has been successfully established. The amino acid analysis procedure using precolumn derivatization was simple and the chromatography was very accurate. However, derivatized samples were sensitive to light and ambient temperature. This problem may impede the development of an automated system for the analysis. Reliability of the newly developed procedures for both hydrolysis and amino acid analysis are being tested and compared with reference checks from other laboratories.

Impacts
(N/A)

Publications