Source: WASHINGTON STATE UNIVERSITY submitted to NRP
A NEW DOUBLE WAVE LENGTH COLORIMETRIC DIFFERENTIAL TANNIN
Sponsoring Institution
Agricultural Research Service/USDA
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
0408733
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
Sep 15, 2004
Project End Date
May 31, 2006
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
WASHINGTON STATE UNIVERSITY
240 FRENCH ADMINISTRATION BLDG
PULLMAN,WA 99164-0001
Performing Department
NATURAL RESOURCE SCIENCES
Non Technical Summary
(N/A)
Animal Health Component
50%
Research Effort Categories
Basic
50%
Applied
50%
Developmental
0%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
20511311020100%
Knowledge Area
205 - Plant Management Systems;

Subject Of Investigation
1131 - Wine grapes;

Field Of Science
1020 - Physiology;
Goals / Objectives
Provide the NW wine processing industry with a rapid and low-cost quality control technology for continuous monitoring of the integral sensory quality of maturing red wine. Elucidate the oxidative capacity of polymeric anthocyanins vs. condensed tannins at different stages of wine maturing as well as their individual antioxidant health-benefit properties related to color, astringency, and bitterness.
Project Methods
Use a new rapid and sensitive double-wavelength colorimetric differential tannin-anthocyanins simultaneous biochemical assay to quantitatively describe the quality of interaction of various sensory constituents in maturing red wine, depending on oxygen availability. This method will be used for preparative separation of condensed tannin and pigments to facilitate individual characterization. The oxidation potential and antioxidant activity of maturing red wine will be monitored by our original method (Schenk and Silber, 2001, Patent Pending) based on both biochemical and electrochemical detection and measurement of peroxidase (PO) coupled to superoxide dismutase (SOD) activities. Documents Grant with WSU.

Progress 09/15/04 to 05/31/06

Outputs
Progress Report 4d Progress report. This report serves to document research conducted under a grant agreement between ARS and Washington State University. Additional details of research can be found in the report for the parent project 5358-21000-034- 00D Production Systems to Promote Yield and Quality of Grapes in the Pacific Northwest. Dr. Morris Silber and collaborators conducted the following research towards the agreements objectives: Objective 1. To provide the NW wine processing industry with a novel rapid and low-cost tannin/anthocyanin technology for continuous monitoring of the integral sensory quality index (ISQI) of maturing red wine. The index reflects the complex interactive relationship in maturing red wine of the Redox potential and various sensory factors (color, bitterness, astringency, acidity, and alcoholic strength). Objective 2. To elucidate the oxidative capacity of polymeric anthocyanins vs. condensed tannins at different stages of wine maturing, as well as their individual antioxidant health-benefit properties related to color, astringency, and bitterness. Our laboratory at Washington State University has developed (Silber, 2002, Patent Pending) a new bioassay which allows preparative separation of condensed tannin and pigments in one assay. Accomplishments: 1. A schematic chart was originally designed that translates the complex interactive relationship of various sensory factors with O2 availability and the Redox activity in red wine during its maturation. 2. Results from the pilot testing were obtained that supported the original concept and validated the informative relevance of the ISQI at different stages of red wine maturation. 3. Results from the Phase I main experiment indicated that the ISQI closely reflected the inverse dynamics of the level changes in condensed tannin and polymeric anthocyanins (about 25% decrease in reactive condensed tannin and a 35% increase in polymeric anthocyanins) along with red wine maturation. 4. Results from the Phase II main experiment indicated that the antioxidant activity in red wine was inherently pertinent to the high polymerized moieties of the tannin and pigments. 5. The results of the research have been presented at the Annual NCSFR Conference and at a special session on "Plant-derived medicinal and dietary products: quality, efficacy, and safety" at the Annual AAAS Symposium. They can also be viewed at our web page tannin.wsu.edu. A peer- reviewed publication is currently in press.

Impacts
(N/A)

Publications


    Progress 10/01/04 to 09/30/05

    Outputs
    4d Progress report. This report serves to document research conducted under a grant agreement between ARS and Washington State University. Additional details of research can be found in the report for the parent project 5358-21000-034- 00D Production Systems to Promote Yield and Quality of Grapes in the Pacific Northwest. Objective 1. To provide the NW wine processing industry with a novel rapid and low-cost tannin/anthocyanin technology for continuous monitoring of the integral sensory quality index (ISQI) of maturing red wine. The index reflects the complex interactive relationship in maturing red wine of the Redox potential and various sensory factors (color, bitterness, astringency, acidity, and alcoholic strength). Objective 2. To elucidate the oxidative capacity of polymeric anthocyanins vs. condensed tannins at different stages of wine maturing, as well as their individual antioxidant health-benefit properties related to color, astringency, and bitterness. Our laboratory at Washington State University has developed (Silber, 2002, Patent Pending) a new bioassay which allows preparative separation of condensed tannin and pigments in one assay. Accomplishments: 1. A schematic chart was originally designed that translates the complex interactive relationship of various sensory factors with O2 availability and the Redox activity in red wine during its maturation. 2. Results from the pilot testing were obtained that supported the original concept and validated the informative relevance of the ISQI at different stages of red wine maturation. 3. Results from the Phase I main experiment indicated that the ISQI closely reflected the inverse dynamics of the level changes in condensed tannin and polymeric anthocyanins (about 25% decrease in reactive condensed tannin and a 35% increase in polymeric anthocyanins) along with red wine maturation. 4. Results from the Phase II main experiment indicated that the antioxidant activity in red wine was inherently pertinent to the high polymerized moieties of the tannin and pigments. 5. The results of the research have been presented at the Annual NCSFR Conference and at a special session on "Plant-derived medicinal and dietary products: quality, efficacy, and safety" at the Annual AAAS Symposium. They can also be viewed at our web page tannin.wsu.edu. A peer- reviewed publication is currently in press.

    Impacts
    (N/A)

    Publications