Progress 10/01/08 to 09/30/09
Outputs
Progress Report Objectives (from AD-416) The objective of this cooperative research project is to 1) characterize the microbial gut flora of Lygus lineolaris and Euschistus servus, 2) identify environmental factors (e.g., host plants, parasitism, time and location of collection, insecticides) that affect the gut flora of these bugs, and 3) determine the role of the microbial gut flora on the survival of the hosts. Approach (from AD-416) Lygus linolaris and Euschistus servus will be collected from different host plants and locations in the Mississippi Delta from April through October. Bugs will also be collected from insecticide-treated cotton. Guts will be dissected and culturable bacteria will be plated on several types of media. Populaiton counts will be done by acridine orange direct count. Molecular analysis of culturable bacteria will facilitate identification via the NCBI database. Unculturable bacteria will be identified via denaturing gradient gel electrophoresis. Antibiotics and other treatments will be used to manipulate the microbial flora of the guts, and the effects on host longevity, fecundity, and other life history traits will be evaluated. Significant Activities that Support Special Target Populations Progress concluded with the 2008 annual report, but was not terminated because of financial management reasons. See 2008 Annual Report for last reported progress.
Impacts
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Progress 10/01/06 to 09/30/07
Outputs
Progress Report Objectives (from AD-416) The objective of this cooperative research project is to 1) characterize the microbial gut flora of Lygus lineolaris and Euschistus servus, 2) identify environmental factors (e.g., host plants, parasitism, time and location of collection, insecticides) that affect the gut flora of these bugs, and 3) determine the role of the microbial gut flora on the survival of the hosts. Approach (from AD-416) Lygus linolaris and Euschistus servus will be collected from different host plants and locations in the Mississippi Delta from April through October. Bugs will also be collected from insecticide-treated cotton. Guts will be dissected and culturable bacteria will be plated on several types of media. Populaiton counts will be done by acridine orange direct count. Molecular analysis of culturable bacteria will facilitate identification via the NCBI database. Unculturable bacteria will be identified via denaturing gradient gel electrophoresis. Antibiotics and other treatments will be used to manipulate the microbial flora of the guts, and the effects on host longevity, fecundity, and other life history traits will be evaluated. Significant Activities that Support Special Target Populations This report serves to document research conducted under a Specific Cooperative Agreement between University of Colorado and ARS. Additional details for this subordinate project may be found in the in-house project 6402-22000-045-00D, �Integrated Pest Management for Mid-South Area Row Crops.� Characterizing the microbial gut flora of the tarnished plant bug and brown stink bug with the environmental factors that affect it. This agreement was monitored by one conference call and two site visits.
Impacts
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Progress 06/15/04 to 08/31/07
Outputs
Progress Report Objectives (from AD-416) The objective of this cooperative research project is to 1) characterize the microbial gut flora of Lygus lineolaris and Euschistus servus, 2) identify environmental factors (e.g., host plants, parasitism, time and location of collection, insecticides) that affect the gut flora of these bugs, and 3) determine the role of the microbial gut flora on the survival of the hosts. Approach (from AD-416) Lygus linolaris and Euschistus servus will be collected from different host plants and locations in the Mississippi Delta from April through October. Bugs will also be collected from insecticide-treated cotton. Guts will be dissected and culturable bacteria will be plated on several types of media. Populaiton counts will be done by acridine orange direct count. Molecular analysis of culturable bacteria will facilitate identification via the NCBI database. Unculturable bacteria will be identified via denaturing gradient gel electrophoresis. Antibiotics and other treatments will be used to manipulate the microbial flora of the guts, and the effects on host longevity, fecundity, and other life history traits will be evaluated. Significant Activities that Support Special Target Populations Characterizing the microbial gut flora of the tarnished plant bug and brown stink bug with the environmental factors that affect it. This agreement was monitored by one conference call and two site visits.
Impacts
(N/A)
Publications
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Progress 10/01/05 to 09/30/06
Outputs
Progress Report 4d Progress report. This report serves to document research conducted under a Specific Cooperative Agreement between University of Colorado and ARS. Additional details for this subordinate project may be found in the inhouse project, 6402-22000-045-00D, entitled, "Integrated Pest Management for Mid-South Area Row Crops." Characterizing the microbial gut flora of the tarnished plant bug and brown stink bug with the environmental factors that affect it.
Impacts
(N/A)
Publications
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Progress 10/01/04 to 09/30/05
Outputs
4d Progress report. This report serves to document research conducted under a Specific Cooperative Agreement between University of Colorado and ARS. Additional details for this subordinate project may be found in the in-house parent project 6402-22000-034-00D entitled, "Insect Pest Suppression Strategies to Improve Production of Corn, Cotton, Soybean, and Sweetpotato in Mid South." Characterizing the the microbial gut flora of the tarnished plant bug and brown stink bug with the environmental factors that affect it. Results of this agreement are pending until it ends next year.
Impacts
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