Source: AGENCOURT BIOSCIENCE CORPORATION submitted to NRP
SEQUENCING BOVINE BAC CLONES FORM RPCI-42 LIBRARY
Sponsoring Institution
Agricultural Research Service/USDA
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
0408618
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
Jun 1, 2004
Project End Date
Jun 30, 2004
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
AGENCOURT BIOSCIENCE CORPORATION
100 CUMMINGS CENTER, STE 107G
BEVERLY,MA 01915
Performing Department
(N/A)
Non Technical Summary
(N/A)
Animal Health Component
40%
Research Effort Categories
Basic
50%
Applied
40%
Developmental
10%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
2123310109030%
2123310111030%
2123410109020%
2123410111020%
Goals / Objectives
Sequence both ends of approximately 17,280 BAC clones from selected bovine BAC clone libraries.
Project Methods
Approximately 17,280 clones selected from bovine BAC clone libraries will be sequenced at both ends at a quality of at least 400 bases with a Phred score of 20 or greater for each BAC end. The resulting information will be supplied to the British Columbia Genomic Center for incorporation into the existing database of BAC clone squences and submitted to Genbank.

Progress 06/01/04 to 06/30/04

Outputs
4d Progress report. This report serves to document research conducted under a specific cooperative research agreement with the Agencourt Bioscience Corporation the goal of which is to provide a framework for the sequencing of the bovine genome. Additional details of the research can be found in the report for the parent project 1265-32000-072-00D "Immunological and genetic basis of resistance to parasites of cattle". Agencourt produced sequences from 45 384-well plates, which were processed at BFGL for further bioinformatic analysis. A total of 25,454 BAC end sequences with corresponding trace files were provided to the International Bovine BAC Map Consortium and the NCBI Trace Archive to help improve the BAC map and assembly of the bovine genome draft sequence. The citation for submission to GenBank is as follows: Sonstegard, T.S., Van Tassell, C.P., Capuco, A.V., de Jong, P., Matukumalli, L.K., Shade, L.S., Bosak, S., Rubenfield, M. and Gasbarre, L. C. 2005. Bovine BAC End Sequences from Library RPCI-42. CZ404298-429751.

Impacts
(N/A)

Publications