Source: AGRICULTURAL RESEARCH SERVICE submitted to NRP
SINGLE NUCLEOTIDE POLYMORPHISM (SNP) DISCOVERY & MAPPING
Sponsoring Institution
Agricultural Research Service/USDA
Project Status
COMPLETE
Funding Source
USDA INHOUSE
Reporting Frequency
Annual
Accession No.
0407158
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
Apr 1, 2003
Project End Date
Mar 31, 2004
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
AGRICULTURAL RESEARCH SERVICE
RM 331, BLDG 003, BARC-W
BELTSVILLE,MD 20705-2351
Performing Department
(N/A)
Non Technical Summary
(N/A)
Animal Health Component
0%
Research Effort Categories
Basic
100%
Applied
0%
Developmental
0%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
2031549104010%
2031820104090%
Knowledge Area
203 - Plant Biological Efficiency and Abiotic Stresses Affecting Plants;

Subject Of Investigation
1549 - Wheat, general/other; 1820 - Soybean;

Field Of Science
1040 - Molecular biology;
Goals / Objectives
The first objective is the development of DNA markers called Single Nucleotide Polymorphisms or SNPs to speed soybean variety development using marker assisted selection. The second objective is to map the newly developed SNP markers on the of Univ. of Utah soybean genome map.
Project Methods
The development of SNP DNA markers will focus on the discovery of markers from 1) Monsanto BAC-end sequence data and 2) Expressed Sequence Tag (EST) DNA sequence data generated by the USDA, Ames, IA with N.C. Region Soybean Research funding. Polymerase chain reaction primers will be developed using these two sources of soybean DNA sequence data. The SNP markers will be developed via the sequence analysis of a set of six previously identified soybean genotypes which are particularly useful for the discovery of SNPs. Genetic mapping in two of the University of Utah recombinant inbred line mapping populations will be used to position the markers on the soybean genome map along with more than 1000 SSR markers that have been previously mapped. Using this approach as well as targeted marker development we will identify additional SNP markers in close proximity to genes that provide resistance to Phytophthora root rot, foliar insect feeding, and elevated seed protein in order to select new varieties with improved resistance and seed quality characteristics.

Progress 04/01/03 to 03/31/04

Outputs
4. What were the most significant accomplishments this past year? D. Progress Report (OPTIONAL FOR ALL IN-HOUSE PROJECTS AND THE PROJECTS LISTED IN APPENDIX A; MANDATORY FOR SUBORDINATE PROJECTS - note change from previous year) This report serves to document research under a reimbursable agreement with the United Soybean Board (Grant #3212) and supports the objectives of project 1275-21000-164-00D. Additional details of the research can be found in the report for the parent project 1275-21000-164-00D (A Single Nucleotide Polymorphism-based Map of Soybean and Applications to Gene Discovery in Germplasm). The work is conducted in collaboration with researchers at the University of Nebraska, Lincoln; the USDA/Iowa State University; and the University of Utah, Salt Lake City. Work was conducted to develop Single Nucleotide Polymorphism (SNP) DNA markers using soybean DNA sequence data from soybean unigene sequence derived from expressed sequence tags (ESTs). SNP DNA markers discovered from these sources are being mapped on the existing soybean genetic map using the Univ. of Utah Minsoy x Noir 1 mapping population at the University of Nebraska, Lincoln.

Impacts
(N/A)

Publications