Progress 07/22/02 to 05/01/07
Outputs
Progress Report Objectives (from AD-416) To sequence and annotate the Babesia bovis genome and use this information for gene discovery to develop a vaccine for bovine babesiosis. Approach (from AD-416) A virulent isolate of Babesia bovis (Texas isolate) will be transmitted to spleen intact calves (demonstrating tick transmissibility) and isolated in erythrocyte culture. Erythrocyte cultures will be used to avoid DNA contamination with bovine leukocyte DNA. Genomic DNA of B. bovis will be isolated from cultured parasites and prepared for insertion into vector pBELOBAC11 and electroporated into E. coli strain DH10B. This BAC library will be screened for bovine DNA contamination through filter hybridizations. A second parallel genomics approach will include shotgun sequencing of the B. bovis genome. This two-strategy approach will allow for gene discovery using the BAC library while we are working through the shotgun approach. Also, the BAC library will be invaluable in sorting out regions of the genome with repeat sequences. The overall strategy is to use this information to discover which genes are expressed as the organism adapts to the tick vector and which genes are expressed as the parasite leaves the tick vector. BSL-1; 6-30-05. Documents SCA with Wash State Univ. Formerly 5348-32000-020-01S. (January 2007) Significant Activities that Support Special Target Populations This report serves to document research conducted under a specific cooperative agreement between ARS and Washington State University. Additional details of research can be found in the report for the parent project 5348-32000-028-00D Immunologic and Pharmacological Interventions of Vector-Borne Babesiosis. Through this agreement and a subsequent agreement we have collaboratively completed sequencing and annotation of the Babesia bovis genome. This work represents collaboration between ARS, TIGR and WSU. A manuscript describing this work is under review at PLOS (Public Library of Science). These data are critical to our strategy to build transfection based vaccines for vector borne diseases. A second manuscript describing the comparative genomics of plastid-like organelles referred to as apicoplast is under review. The ADODR participated in weekly onsite meetings during the period of this report.
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Progress 10/01/05 to 09/30/06
Outputs
Progress Report 4d Progress report. This report serves to document research conducted under a specific cooperative agreement between ARS and Washington State University. Additional details of research can be found in the report for the parent project 5438-32000-020- 00D Functional Approaches to the Control of Babesiosis in Cattle and Horses. The goal of this collaboration is the development of safe and efficacious vaccines for bovine and equine babesiosis, with a focus on transmission blocking vaccines (host to tick vector). Using data derived from the Babesia bovis genome specific sites (rDNA) we identified for site directed transfection.
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Progress 10/01/04 to 09/30/05
Outputs
4d Progress report. This report serves to document research conducted under a specific cooperative agreement between ARS and Washington State University. Additional details of research can be found in the report for the parent project 5438-32000-020-00D Functional Approaches to the Control of Babesiosis in Cattle and Horses. The goal of this collaboration is the development of safe and efficacious vaccines for bovine and equine babesiosis, with a focus on transmission blocking vaccines (host to tick vector). During this past year we obtained 8X coverage of the Babesia bovis genome and have closed 3 of the 4 chromosomes. During this research we discovered data concerning the origin of Apicomplexan plastids. A manuscript describing these findings is under consideration by PNAS.
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Progress 10/01/03 to 09/30/04
Outputs
4. What were the most significant accomplishments this past year? D. This report serves to document research conducted under a specific cooperative agreement between ARS and Washington State University. The goal of this collaboration is the development of safe and efficacious vaccines for bovine and equine babesiosis, with a focus on transmission blocking vaccines (host to tick vector). During this past year we obtained 8X coverage of the Babesia bovis genome. We are now using these data for gene discovery through comparative genomics with the available Plasmodium falciparum genome (a causative agent of malaria). A number of potential vaccine targets have been identified including one which is possibly sporozoite stage specific. Additional details of research can be found in the report for the parent project 5438-32000-020-00D Animal Health.
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