Progress 09/28/01 to 02/28/05
Outputs
4d Progress report. This report serves to document research conducted under a specific cooperative research agreement between ARS and the University of Hawai'i at Manoa. Additional details of research can be found in the report of the associated in-house project 5320-22430-020-00D, Biology, Control and Areawide Management of Fruit Flies and Other Pests. No further research was conducted under this agreement in FY2005. However, further effort was made to relate the results of this research to the overall tephritid fruit fly male lure pattern. The identification of eugenol as a synergist for the attractiveness of alpha-ionol to B. latifrons helps to better place B. latifrons in the overall Dacine male lure response pattern - showing greater affinity of this species to methyl eugenol - responding species than to cuelure - responding species.
Impacts
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Progress 10/01/02 to 09/30/03
Outputs
4. What were the most significant accomplishments this past year? This report serves to document research conducted under a specific cooperative research agreement 5320-22430-020-05S Improvement of Attractants for Tephritid Fruit Flies, between ARS and the University of Hawaii Department of Molecular Biosciences Bioengineering. Additional details of research can be found in the report for the parent CRIS 5320- 22430-020-00D Biology, Control, and Areawide Management of Fruit Flies and Other Pests. The research over the past year focused on field assessment of seven compounds for synergistic enhancement of attraction of alpha-ionol to male solanaceous fruit fly (Bactrocera latifrons). These compounds had previously been identified as potential active ingredients in cade oil which is known to be an alpha-ionol synergist. Using releases of sterile flies, four of the potential active ingredients (eugenol, isoeugenol, 2-methoxy-4-ethyl phenol, and 2-methoxy-4-propyl
phenol), were found to be capable of synergizing the attractiveness of alpha-ionol to male B. latifrons under field conditions. The similar structure of these four identified synergistic compounds shows that there is a response to a basic 2-methoxy phenol structure, with fly response little affected by some variation in the composition of the side chain on the number 4 carbon. Because identified synergists were structurally quite similar only one compound (eugenol) was selected for further field studies with wild flies. Selection was based on relative attraction, perceived relative safety of use and cost considerations. Eugenol and alpha-ionol, however, were unable to provide attraction comparable to that of cade oil and alpha-ionol in tests with wild fly populations. Although the research has not, yet, resulted in an improved male lure, the identification of active components of cade oil provides further insight into the male attractant for B. latifrons and opens additional
paths of research which could lead to improvement of the male lure for B. latifrons.
Impacts
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