Progress 10/01/00 to 09/30/05
Outputs
4d Progress report. This report serves to document research conducted under a specific cooperative agreement between ARS and University of Tennessee. Additional details of research can be found in the report for the parent project 6645-21000-025-00D, Fundamental mechanisms for genetic alteration of soybean quality and productivity. The objective of this research is to find places (DNA markers) in the soybean genome where genes are located that control protein quantity and quality. Two DNA markers were discovered which are close to the genes that govern the two main components, termed 7S and 11S, of soybean protein. A marker for glycinin (the main soybean protein) is located near position 14.0 on the G gene linkage group of the soybean genome. These markers will be an aid in breeding soybeans that have more 11S protein. Because the 11S portion of the protein contains about 3 to 4 times more of the amino acids, methionine and cysteine, than the 7S portion, such
information may lead to future improvements in soybean protein by increasing these amino acids. Soybean protein is deficient in metionine and cysteine, which are needed in animal and human diets.
Impacts
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Progress 10/01/03 to 09/30/04
Outputs
4. What were the most significant accomplishments this past year? This report serves to document research conducted under a specific cooperative agreement between ARS and the Agricultural Research Station, University of Tennessee. Additional details of research can be found in the report for the parent project 6645-21000-025-00D, Fundamental mechanisms for genetic alteration of soybean quality and productivity. Eight minor quantitative trait gene loci (QTL) were detected for methionine (on linkage groups A1, A2, D1a, E, G, K, and H) and one major QTL on LG A1 near positon 93.2 by marker Satt236. Four minor QTL for cysteine (on LG A1, C1, and L) and a major QTL on LG K near position 46.2 by marker Satt417 were detected during this year. The sulfur-containing amino acids, cysteine and methionine, are important components of soybean protein quality and nutrition. 101 inbred lines were grown in four-row plots over three locations. Variation in cysteine and
methionine was normally distributed. Molecular markers associated with methionine and cysteine will aid in a more efficient breeding program for increased sulfur amino acids in soybean.
Impacts
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