Progress 10/01/04 to 09/30/05
Outputs
4d Progress report. This is a final report for this project. This report serves to document research conducted under a specific cooperative agreement between ARS and The University of Missouri. Additional details can be found in the report for the parent CRIS 1940-32000-040-00D "Pathogenesis and Genomics of Vesicular Stomatitis Viruses and Foot and Mouth Disease". This project is in collaboration with the School of Veterinary Medicine, University of Missouri-Columbia. The purpose of this collaborative research is to gain a better understanding of the nature and quality of the immune responses to foot-and-mouth disease (FMD) and vesicular stomatitis (VSV) viruses in cattle. It includes assessment of the humoral and cytokine immune response components as well as T dependent vs. T-independent qualities of the humoral response to viral infections and vaccination in bovine. We amended this agreement to include the joint development of monoclonal antibody reagents for
detection of bovine cytokines, particularly type I interferons. This work has resulted in the production of monoclonal antibodies currently under testing at PIADC. In addition we have continued studies using a model of pathogenesis in cattle that allowed tracking of the viral infection from the inoculation site to the regional lymph node. We determined the primary site of replication in cattle and the local skin cytokine response to VSV inoculation. This information is relevant to determining the menchanisms of disease and transmission of VSV. Other activities in 2005 were limited to finalizing activities and transitioning to a new collaborative agreement established with the University of Texas Medical Branch.
Impacts
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Progress 04/01/01 to 03/31/05
Outputs
Progress Report 4d Progress report. This is a final report for this project. This report serves to document research conducted under a specific cooperative agreement between ARS and The University of Missouri. Additional details can be found in the report for the parent project 1940-32000-040-00D "Pathogenesis and Genomics of Vesicular Stomatitis Viruses and Foot and Mouth Disease". The purpose of this collaborative research is to gain a better understanding of the nature and quality of the immune responses to foot-and-mouth disease (FMD) and vesicular stomatitis (VSV) viruses in cattle. It includes assessment of the humoral and cytokine immune response components as well as T dependent vs. T-independent qualities of the humoral response to viral infections and vaccination in bovine. A study was carried out to gain a better understanding of the systemic T- helper cell balance mediating the immune response against VSV in cattle. The results suggest that VSV antigen
induces a T-helper Type 1 response in both infected and vaccinated cattle. We propose that analysis of cellular responses at the sites of lesion and in draining lymph nodes, would give a more accurate picture of the events associated with development of immunity to this localized infection in domestic animals. We amended this agreement to include the joint development of monoclonal antibody reagents for detection of bovine cytokines, particularly type I interferons. This work has resulted in the production of monoclonal antibodies currently under testing at PIADC. In addition, we have continued studies using a model of pathogenesis in cattle that allowed tracking of the viral infection from the inoculation site to the regional lymph node. We determined the primary site of replication in cattle and the local skin cytokine response to VSV inoculation. This information is relevant to determining the mechanisms of disease and transmission of VSV. This research falls within the component
1: Biodefense Research of the NP-103 National Program.
Impacts
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Progress 10/01/03 to 09/30/04
Outputs
4. What were the most significant accomplishments this past year? D. Progress Report: This report serves to document research conducted under a specific cooperative agreement between ARS and The University of Missouri. Additional details can be found in the report for the parent CRIS 1940- 32000-040-00D "Pathogenesis and Genomics of Vesicular Stomatitis Viruses and Foot and Mouth Disease". This project is in collaboration with the School of Veterinary Medicine, University of Missouri-Columbia. The purpose of this collaborative research is to gain a better understanding of the nature and quality of the immune responses to foot-and-mouth disease (FMD) and vesicular stomatitis (VSV) viruses in cattle. It includes assessment of the humoral and cytokine immune response components as well as T-dependent vs. T-independent qualities of the humoral response to viral infections and vaccination in bovine. During the last year we amended this agreement to include the joint
development of monoclonal antibody reagents for detection of bovine cytokines, particularly type I interferons. During the last year we have used quantitative real-time PCR assays developed during the previous year to study the pathogenesis of VSV infection and vaccination in cattle. We have also developed a model of pathogenesis in cattle that allowed tracking of the viral infection from the inoculation site to the regional lymph node and determined the local skin response to VSV inoculation including quantitation of interferon alpha, beta and gamma, IL-2, IL-4 and TNF-alpha. Using these assays we are now dissecting the events leading to lesion induction after VSV infection.
Impacts
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