Progress 09/01/00 to 08/31/05
Outputs
4d Progress report. This report serves as the final report for research conducted under a specific cooperative agreement between ARS and Colorado State University. Additional details can be found in the report for the parent CRIS 1940- 32000-040-00D "Pathogenesis and Genomics of Vesicular Stomatitis Viruses and Foot and Mouth Disease". This project is in collaboration with the Center for Veterinary Epidemiology and Animal Disease Surveillance Systems (CVEADSS) at the Department of Environmental Health at Colorado State University, Fort Collins, Colorado (CSU). The purpose of this collaborative research is to investigate epidemiological relationships of VSV and use knowledge gained to better understand the life cycle of VSV. An amendment has been made to this project in order to include a new objective: to assist ARS in the evaluation and validation of rapid diagnostic real-time PCR for VSV. In the field VSV epidemiological study in endemic areas of Mexico, a large
amount of data has been collected and are still being analyzed. But over the life of the project it the endemic activity of VSV in southern Mexico was clearly documented. Additional important differences were observed in temporal and geographical distribution of infections between different ecological zones. Most infections occurred with the rainy season and during times when the blood-feeding insects were most commonly found. In addition samples from vsv-negative cattle in the state of Colorado, were collected in order to validate a rapid diagnostic test based on real-time RT-PCR. A total of 1534 cattle from 30 different operations were sampled for this study by July 19th, 2005. The criterion for selection of these operations was based on the owner's willingness to collaborate with this study and their location in the State. The operations were located in the north east of Colorado, far from the only one case of VSV in cattle registered in Montrose County (South West) in July 2005.
Oral swabs were collected from 1529 cattle and serum samples were collected from all 1534 animals. These samples were submitted to the California Animal Health and Food Safety Laboratory in Davis for testing. Testing of the first 700 sampels did not show any false positive results. Testing is ongoing at UC Davis. Analysis of the data collected at study sites on VSV-endemic areas of Mexico has resulted in the preparation of a manuscript entitled Geographic and Environmental Factors Associated to Vesicular Stomatitis Outbreaks In Mexico (1981-2000) that has been submitted for publication.
Impacts
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Progress 10/01/03 to 09/30/04
Outputs
4. What were the most significant accomplishments this past year? D. Progress Report: This report serves to document research conducted under a specific cooperative agreement between ARS and Colorado State University. Additional details can be found in the report for the parent CRIS 1940- 32000-040-00D "Pathogenesis and Genomics of Vesicular Stomatitis Viruses and Foot and Mouth Disease". This project is in collaboration with the Center for Veterinary Epidemiology and Animal Disease Surveillance Systems (CVEADSS) at the Department of Environmental Health at Colorado State University, Fort Collins, Colorado (CSU). The purpose of this collaborative research is to investigate epidemiological relationships of VSV and use knowledge gained to better understand the life cycle of VSV. An amendment has been made to this project in order to include a new objective: to assist ARS in the evaluation and validation of rapid diagnostic real-time PCR for VSV. Current status of the
collection of samples from vsv-negative cattle in the state of Colorado, USA: A total of 527 cattle from 13 different operations (dairy and beef types) were sampled for this project as of July 15th, 2004. Oral swabs and serum samples were collected from all 527 animals. In addition, hoof swabs were collected from 46 adult dairy cows. A total of 1100 samples (oral swabs, hoof swabs and serum samples) were collected, identified, and stored at -70oC according to the approved standard operating procedures. A database containing information on individual farms were completed and are available upon request. Production data and other characteristics of the tested animals, such as presence or absence of VSV-like lesions, were recorded in the database if available at the farm, as described in the original protocol. The collection of samples will continue in dairy operations from Colorado until sample size requirements are satisfied. Analysis of the data collected at study sites on
VSV-endemic areas of Mexico has resulted in the preparation of a manuscript entitled "Geographic and Environmental Factors Associated to Vesicular Stomatitis Outbreaks In Mexico (1981-2000)" that will be submitted for publication.
Impacts
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Publications
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