Source: MICHIGAN STATE UNIV submitted to NRP
MICROSATELLITE MARKER DEVELOPMENT & CONSTRUCTION OF MICROSATELLITE ALLELE SIZE DATABASE
Sponsoring Institution
Agricultural Research Service/USDA
Project Status
COMPLETE
Funding Source
USDA COOPERATIVE AGREEMENT
Reporting Frequency
Annual
Accession No.
0403377
Grant No.
58-1275-0-024
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
Apr 1, 2000
Project End Date
Mar 31, 2005
Grant Year
2000
Program Code
[(N/A)]- (N/A)
Recipient Organization
MICHIGAN STATE UNIV
(N/A)
EAST LANSING,MI 48824
Performing Department
(N/A)
Non Technical Summary
(N/A)
Animal Health Component
30%
Research Effort Categories
Basic
70%
Applied
30%
Developmental
0%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
2031549104010%
2031820104080%
2121820104010%
Knowledge Area
212 - Pathogens and Nematodes Affecting Plants; 203 - Plant Biological Efficiency and Abiotic Stresses Affecting Plants;

Subject Of Investigation
1549 - Wheat, general/other; 1820 - Soybean;

Field Of Science
1040 - Molecular biology;
Goals / Objectives
The first onbjective is to map between 200 and 250 new microsatellite markers in the wheat genome via recombination mapping in the International Tritici Mapping Initiative (ITMI) mapping population. The second objective is to use a set of selected microsatellite markers to characterize the potent scab resistant genotypes Sumai 3, Ning7840 and W14 along with a panel of elite U.S. breeding parents.
Project Methods
DNA of the parents and the 105 recombinant inbred lines (RILs) of the ITMI mapping population will be isolated. Newly developed microsatellite markers that are polymorphic in the ITMI mapping population will be sent to Michigan State Univ. and will be positioned in the wheat genome via recombination mapping in the ITMI RILs. DNA will be isolated from the scab resistanct cultivars Sumai 3, Ning7840 and W14 as well as a panel of 36 hexaploid and durum wheat breeding parents identified by breeding programs in scab-threatened areas of the U.S. Mapped microsatellite loci will be assigned to one of 84 arm sectors (2 per chromosome arm one distal and one proximal to the centromere). Allelic states for the two loci in each of the arm sectors will be determined for each accession using fluorescent labelled nucleotides analyzed on a Perkin-Elmer 377 DNA sequencer.

Progress 04/01/00 to 03/31/05

Outputs
4d Progress report. This report serves to document research conducted under a specific cooperative agreement with the Department of Crop and Soil Sciences, Michigan St. Univ. and is a subcontract derived from funding received from the U.S. Wheat and Barley Scab Initiative and supports the objectives of project 1275-21000-164-00D. Additional details of the research can be found in the reports for the parent project 1275-21000- 164-00D (A Single Nucleotide Polymorphism-based Map of Soybean and Applications to Gene Discovery in Germplasm). During the reporting period a new map of hexaploid wheat was published that contains a total of 540 new simple sequence repeat (SSR) DNA markers that were developed at the USDA, Beltsville. Of these 347 were mapped to positions on the 21 wheat chromosomes by genetic mapping in the ITMI (International Tritici Mapping Initiative) mapping population at Michigan St. Univ. and/or by deletion mapping at Kansas St. Univ. The newly enlarged genetic map of the wheat genome is an important resource for wheat geneticists and will be used to discover and introgress genes affecting important biotic and abiotic stress resistance, wheat quality characteristics as well as yield per se.

Impacts
(N/A)

Publications


    Progress 10/01/03 to 09/30/04

    Outputs
    4. What were the most significant accomplishments this past year? D. Progress Report (OPTIONAL FOR ALL IN-HOUSE PROJECTS AND THE PROJECTS LISTED IN APPENDIX A; MANDATORY FOR SUBORDINATE PROJECTS - note change from previous year) This report serves to document research conducted under a specific cooperative agreement with the Department of Crop and Soil Sciences, Michigan St. Univ. and is a subcontract derived from funding received from the U.S. Wheat and Barley Scab Initiative and supports the objectives of project 1275-21000-164-00D. Additional details of the research can be found in the reports for the parent project 1275-21000- 164-00D (A Single Nucleotide Polymorphism-based Map of Soybean and Applications to Gene Discovery in Germplasm). During the reporting period a new map of hexaploid wheat was developed that contains a total of 540 new simple sequence repeat (SSR) DNA markers that were developed at the USDA, Beltsville. Of these 347 were mapped to positions on the 21 wheat chromosomes by genetic mapping in the ITMI (International Tritici Mapping Initiative) mapping population at Michigan St. Univ. and/or by deletion mapping at Kansas St. Univ. The newly enlarged genetic map of the wheat genome is an important resource for wheat geneticists and will be used to discover and introgress genes affecting important biotic and abiotic stress resistance, wheat quality characteristics as well as yield per se.

    Impacts
    (N/A)

    Publications