Progress 03/01/13 to 12/31/17
Outputs
Target Audience: The targeted audience include commercial tomato growers, extension agents, researchers, industry personnel, crop consultants, and master gardeners Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?Paret ML,Strayer-Scherer A,Liao YY,Novel copper composites and magnesiumoxide nanomaterials against copper-tolerant strains of Xanthomonas perforans andbacterial spot of tomato. 2017Materials Research Society MRSMeeting Paret ML, Jones JB, Vallad GE. 2017.Management of tomato bacterial spot usingnanomaterials and current approaches to managing bacterial wilt.Material Innovationfor Sustainable Agriculture Annual Meeting, Orlando, FL Liao YY, Strayer-Scherer A, White JC, Mukherjee A, De La Torre-Roche R,Ritchie L, Colee J, Vallad GE, Freeman JH, Jones JB, Paret ML. 2017. Nanomateril magnesium oxide against bacterial spot of tomato.U.S.Tomato Disease Workshop Liao YY, Strayer AL, White JC, Mukherjee A, De La Torre-Roche R, Elmer WH,Young M, Santra S, Ritchie L, Freeman JH, Jones JB, Paret ML. 2017. Magnesiumoxide nanomaterial, a novel bactericide for control of bacterial spot of tomato withoutaccumulating in fruit. American Phytopathological Society Annual Meeting 2017 How have the results been disseminated to communities of interest?Paret ML. 2018. Tomato and cucurbit diseases and management. UF/IFAS Crop Management School, Jasper, FL, January 16, 2018 Paret ML. 2017. Tomato diseases and trial updates for North Florida and South Georgia region. Tomato Forum of the Gadsden County Tomato Growers Association, FL, December 7, 2017. Paret ML. Copper nanomaterials against copper-tolerant strains of Xanthomonas perforans and bacterial spot of tomato. Florida Tomato Committee Annual Meeting, September 8, 2017 What do you plan to do during the next reporting period to accomplish the goals?
Nothing Reported
Impacts
What was accomplished under these goals?
Three novel copper composites with high antibacterial activities against copper tolerant X. perforans strain was discovered based on in vitro studies. Subequentstudies in greenhouse and field conditions demonstrated the activity of these copper composites in effectively reducing bacterial spot of tomato (Strayer et al., 2018). Studies on Magnesium oxide nanomaterials also demonstrated high antibacterial activity against copper-tolerant X. perforans strains in vitro, in greenhouse and in field situation.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2018
Citation:
Strayer A, Liao YY, Young M, Ritchie L, Vallad GE, Santra S, Freeman JH, Clark D, Jones JB, Paret ML. 2018. Advanced copper composites against copper-tolerant Xanthomonas perforans and tomato bacterial spot. Phytopathology 108(2): 196-205
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Progress 10/01/15 to 09/30/16
Outputs
Target Audience: The targeted audience included commercial tomato growers, extension agents, industry personnel, crop consultants and master gardeners Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?The graduate students (Strayer and Liao) who conducted this research presented thisresearch findings as listed below Liao YY, Strayer-Scherer A, White J, Mukherjee A Freeman J, Jones JB, Paret ML.2016.Antibacterial potential of nano-sized magnesium oxide against Xanthomonasperforans causing bacterial spot of tomato.U.S Tomato Disease Workshop Strayer-Scherer A, Liao YY. 2016, Young M, Santra S, Freeman, J, Jones JB, ParetML. 2016.Three novel copper-based nanomaterials for management of bacterial spotof tomato.U.S Tomato Disease Workshop. Strayer-Scherer A, Liao YY, Ocsoy I, Young M, Tan W, Santra S, Jones J, ParetML. 2016. Advanced nanomaterials for management of bacterial spot of tomato.American Phytopathological Society Annual Meeting (Invited forspecial session on nanomaterials). Liao YY, Strayer-Scherer A, White J, Mukherjee A, Elmer W, Ritchie L, Clark D,Freeman J, Jones J, Paret ML. 2016.Antibacterial potential of Magnesium oxidenanomaterial against Xanthomonas perforans causing bacterial spot of tomato.American Phytopathological Society Annual Meeting How have the results been disseminated to communities of interest?Paret ML. 2016. Tomato diseases and trial updates for North Florida and South Georgia region. Tomato Forum of the Gadsden County Tomato Growers Association, FL, December 1, 2016. Paret ML. "Plant pathology: Putting concepts to practice". Gadsden County Master Gardener Training, Quincy, FL, October 25, 2016 Paret ML. 2016. "Disease management of Vegetable Crops: Examples of the most economically relevant pathogen/disease on tomato and cucurbits in North Florida". Panhandle Fruit and Vegetable Conference, Marianna, FL, October 11, 2016 Paret ML. 2016. "An overview of Cu - tolerant plant pathogen management (and potential against another critical plant pathogen) with nanotechnology - enabled pesticides". The Materials Innovation for Sustainable Agriculture Symposium, University of Central Florida, October 3, 2016. Paret ML. "Signs and symptoms of plant problems in the landscape: Examples from ornamentals and tomatoes", Wakulla County Master Gardener Training, Crawfordville, FL, September 8, 2016 What do you plan to do during the next reporting period to accomplish the goals?Complete trials on copper and magnesium nanomaterials and publish research findings and continue on the dissemination activities.
Impacts
What was accomplished under these goals?
A novel DNA-directed silver based nanomaterial was studied for its effectiveness to bacterial spot of tomato under greenhouse conditions. This nanoparticle completelyinhibited growth of copper-tolerant Xanthomonas perforans in vitro at 10 microgram/mL. Greenhouse studies showed that these nanoparticles effectively reduced disease severity in plants compared to non-treated control and grower standard copper-mancozeb (Strayer et al., 2016). Studies on integration of SAR inducer (Acibenzolar S-Methyl) and bio-control agents were conducted which showed its potential as an IPM approach. In addition antibacterial properties of novel copper-composites and magnesium nanomaterials were discovered againstcopper-tolerant Xanthomonas perforans in vitro which was effective in reducing disease severityin greenhouse and under field conditions.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2016
Citation:
Strayer A, Ocsoy I, Tan W, Jones JB, Paret ML. 2016. Low concentrations of a silver-based nanocomposite to manage bacterial spot of tomato in the greenhouse. Plant Disease 100(7): 1460-1465
- Type:
Journal Articles
Status:
Published
Year Published:
2016
Citation:
Strayer A, Jeyaprakash A, Minsavage GV, Timilsina S, Vallad GE, Jones JB, Paret ML. 2016. A multiplex real-time PCR assay differentiates four Xanthomonas species associated with bacterial spot of tomato. Plant Disease 100(8): 1660-1668
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Progress 10/01/14 to 09/30/15
Outputs
Target Audience:> 65 tomato growers, extension agents working with tomato growers,industrypersonnel working with tomato grower, commerical scouts, and master gardeners Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?Paret ML. 2014. Tomato diseases and trial updates for North Florida and SouthGeorgia region 2014. Tomato Forum of the Gadsden County Tomato GrowersAssociation, FL, December 4. A total of 45 growers, extension agents and industrypersonnel attended the talk. Paret ML. 2015. Field identification of tomato diseases. Tomato School, Santa RosaCounty Extension Service, May 19, 2015, Jay, FL. How have the results been disseminated to communities of interest?Through Gadsden county tomato forum and through tomato school, both in panhandle of Florida. What do you plan to do during the next reporting period to accomplish the goals?Continue with demonstration of the risks associated in using copper for bacterial spot managment due tot he development of copper tolerant strains of the bacterium, and continued demonstrationthat the IPM program can be effectively used in bacterial spot managment.
Impacts
What was accomplished under these goals?
The following key accomplishments were made in 2015 Studies on integration of biocontrol agents and SAR inducers showed promise in reducing bacterial spot disease of tomato in field trials conducted in Quincy. This information was shared with tomato growers in Gadsden county, Florida which covers ~2000 acres of production. Majority of the prodcuers practiced an integrated system in 2015 with the use of SAR inducer with biocontrol agents and no reliance on copper for abcterial spot managment which is a significant change from prior years.
Publications
- Type:
Other
Status:
Published
Year Published:
2015
Citation:
Dufault NS, Paret ML, Freeman JH, Olson, SM. 2014. Integrated management of
white mold on vegetables in Florida. EDIS, University of Florida, PP276, p 10.
http://edis.ifas.ufl.edu/pdffiles/PP/PP27600.pdf (Original version released in 2010
first authored by Paret ML).
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Progress 10/01/13 to 09/30/14
Outputs
Target Audience: Target audiences: Growers, extension agents, crop consultants, fungicide industry personnel, master gardeners Efforts: Presentation of latest approaches in bactreial spot managment, communications through extension publications and webistes and blogs, and latest updates through web communications Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided? One of the gradute students involved in this work was able to present work at a University of Flroida program. This work in collaboration with Chemistry group on the project improved knowledge on nanoparticle synthesis and application for the graduate student and the PI of this project. How have the results been disseminated to communities of interest? Two training programs have been conducetd in 2014 to transfer information on bacterial spot disease managment. These were the Gadsden County Tomato Forum, in Florida and the Fall Tomato school at Santa Rosa county, Florida. Website communications through U-scout website of University of Florida helped in connecting to numerous clientele as well as general public. What do you plan to do during the next reporting period to accomplish the goals? One of the key goals for 2015 is to publish research manuscripts on use of nanoparticles against bacterial spot, and one on the development of better detection systems for separating the four Xanthomonas spp associated with bacterial spot disease. We will be also continuing field experimentation of new approaches for bacterial spot disease management.
Impacts
What was accomplished under these goals?
1. Bacterial spot of tomato, caused by four, Xanthomonas spp., is one of the most damaging diseases of tomato that occurs worldwide. Current management strategies used by transplant producers and growers heavily rely on the use of copper-based bactericides, as there are limited options for disease management. A major issue with this practice is the development of copper-resistant strains of the bacteria, which makes copper ineffective. We have undertaken a study to characterize the antibacterial activity of a silver-based nanocomposite, Ag-dsDNA-GO, and its potential as an alternative to copper. In vitro assays using copper-tolerant and sensitive X. perforans strains revealed that Ag-dsDNA-GO at rates as low as 10 ppm killed all bacterial cells within 15 min of exposure. In contrast, the equivalent rates (10, 25, and 50 ppm) of copper were unable to significantly reduce the population of the copper-tolerant strain when compared to the untreated control at 24 h of exposure. All copper concentrations inhibited growth of the copper-sensitive strain, but required exposure for ≥ 1 h. Greenhouse studies on artificially infected transplants treated with either 75 or 100 ppm Ag-dsDNA-GO significantly reduced disease severity when compared with copper-mancozeb and untreated controls. 2. In another study we explored the potential use of a novel SAR compound against bacterial spot which provided promising results in a field trial conducted in 2014. This new material was able to manage bacterial spot disease to comparable levels as the grower standard, copper+mancozeb and significantly reduced disease than the untreated controls.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2014
Citation:
Kebede M, Timilsina S, Ayalew A, Admassu M, Potnis N, Minsavage GV, Goss EM, Hong JC, Strayer A, Paret M, Jones JB, Vallad GE. 2014. Molecular characterization of Xanthomonas strains responsible for bacterial leaf spot of tomato in Ethiopia. European Journal of Plant Pathology 140(4): 677-688
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Progress 03/01/13 to 09/30/13
Outputs
Target Audience: This project findings were extended to the following target audiences during this reporting period 1. Tomato growers, fungicide industry and commercial scouts in Florida through Gadsden County Tomato Growers Association meeting in 2013 2. Tomato growers and extension agents in Florida through field demonstration of the tested technologies during field day at NFREC, University of Florida 3. The research findings were presented at the joint meeting of the 4th International Symposium on tomato diseases and the 28th U.S AnnualTomatoDisease Worskhop held in Orlando, FL in 2013. This meeting was attended by scientists from 19 countries, and industry (Seed/Fungicides). Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided? The project findings on DNA-directed nanoparticles were presented at the joint meeting of the 4th International Symposium on Tomato Diseases and the 27th U.S Annual Tomato Disease Workshop at Orlando, FL in 2013 How have the results been disseminated to communities of interest? The results have been disseminated through grower meetings and fall field days at the University of Florida through demonstrations plots in 2013. What do you plan to do during the next reporting period to accomplish the goals? The following project objectives is expected to be accomplished by the next reporting period. 1. Fine tuning of the photocatalytic nanoparticles and integration with SAR compounds for improved efficacy against bacterial spot on tomato. 2. Improving long-term stability of peracetic acid for field activity. 3. Integration of multiple technologies for managment of bacterial spot on tomato.
Impacts
What was accomplished under these goals?
This project led to the development of two nanomaterials for potential future use as bactericides against bacterial spot on tomato. One of these materials is a photocatalytic nanocompound named AgriTitan based on Titanium dioxide. This material is now under further fine tuning for field application on tomato for bacterial spot management in integration with an SAR compound; Actigard through drip application. Another nanomaterial developed as part of the project was a DNA-directed nanomaterial with high antibacterial properties against X. perforans at 10-16 ppm concentration. This project also led to screening of numerous SAR compounds of which Actigard was highly effective agaianst bacterial spot on tomato by itself as well as in rotation with bio-control agents and copper-based bactericides. In vitro studies on improving field effectiveness of peracetic based materials have been completed.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2013
Citation:
Paret, M. L., Vallad, G. E., Averett, D. R., Jones, J. B., and Olson, S. M. 2013. Photocatalysis: Effect of light-activated nanoscale formulations of TiO2 on Xanthomonas perforans and control of bacterial spot of tomato. Phytopathology 103:228-236.
- Type:
Journal Articles
Status:
Published
Year Published:
2014
Citation:
Ocsoy,I., Paret,M.L., Ocsoy,M.L., Kunwar,S., Chen,T., You,M., and Tan, W.2013. Nanotechnology in Plant Disease Management: DNA-Directed Silver Nanoparticles on Graphene Oxide as an Antibacterial against Xanthomonas perforans. ACS Nano 7:89728980
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