Source: UNIV OF MARYLAND submitted to
MICROBIOLOGICAL ASSESSMENT OF ORGANIC PRODUCE PRE- AND POST-HARVEST AND DEVELOPMENT OF A CUSTOMIZED FOOD SAFETY OUTREACH PROGRAM FOR ORGANIC GROWERS
Sponsoring Institution
State Agricultural Experiment Station
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
0232853
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
Jul 1, 2012
Project End Date
Jun 30, 2013
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
UNIV OF MARYLAND
(N/A)
COLLEGE PARK,MD 20742
Performing Department
Plant Science & Landscape Architecture
Non Technical Summary
Organic crop production is a rapidly growing agricultural sector, but microbiological quality of organic crops, which impacts food safety and produce quality, has not been fully assessed. Data and educational resources specific to organic production, aimed at improving agricultural and handling practices and minimizing human pathogen contamination of crops, are scarce. This research is being proposed to assess the prevalence of Salmonella, Escherichia coli O157:H7 and Listeria monocytogenes on pre- and post-harvest tomatoes, spinach and other fresh produce, as well as irrigation water, on organic farms in Maryland. Indicator bacteria, yeasts and molds will be quantified. Prevalence of two post-harvest disease-causing phytopathogens, Erwinia spp. and Phytophthora infestans, will be assessed to determine whether they are associated with an increased incidence of human pathogens. A training workshop, customized specifically to organic growers' needs in the Mid-Atlantic, will be organized. This workshop will build on an existing University of Maryland Extension Impact Team Program, FoodSmart Good Agricultural Practices workgroup. It will serve as a platform to disseminate findings and strengthen ties with growers, ensuring our research continues to be guided by growers' critical needs.
Animal Health Component
(N/A)
Research Effort Categories
Basic
(N/A)
Applied
(N/A)
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
7121430110025%
7121460110025%
7121499110030%
7121499302010%
7121499303010%
Goals / Objectives
This research aims at assessing the prevalence of foodborne disease-causing bacteria: Salmonella, Escherichia coli O157:H7 and Listeria monocytogenes on pre- and post-harvest fresh produce cultivated on farms adopting organic practices in Maryland. The indicators E. coli, coliforms, aerobic mesophilic bacteria, yeasts and molds, and total microbial load, are also being quantified. A training workshop, customized specifically to organic growers' needs in the Mid-Atlantic, will be organized to reach out to local organic growers and disseminate information tailored to their needs.
Project Methods
Farms will be visited multiple times during the growing season for collection of produce and water samples. Indicator organisms will be enumerated using 3M petrifilms. Pathogens will be isolated by enrichment and selection on appropriate media. Pathogen identification will be verified using multiplex PCR amplification of species-specific genes A method is being developed to measure total bacterial load on produce using quantitative real-time PCR. Workshops at organic grower meetings are being used as a platform for presentation of data and customized information is being disseminated.

Progress 07/01/12 to 06/30/13

Outputs
Target Audience: Growers Extension agents Food safety educators Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? This worked served as a Master's thesis project for one gradaute student.The gradaute student and two undergraduate students were fully trained in experimental design, field sampling, produce and water sample processing for the microbiological assessment of foodborne pathogens and enumeration of indicator microorganisms. The graduate student was also trained in statistical analysis and writing.The gradaute student had the opportunity to also mentor undergraduate students. The graduate student was able to attend a national IAFP conferences and presented a poster on the work that is directly realted to this project. How have the results been disseminated to communities of interest? Treakout Session - Training Workshop: 'GAPs and GHPs for Organic Production,22rd Annual Maryland Organic Food and Farming Association (MOFFA) Winter Meeting 2013,Annapolis MD 'GAPs and GHPs for Organic Production,23rd Annual Maryland Organic Food and Farming Association (MOFFA) Winter Meeting 2014,Annapolis MD Invited Talks 'Good Agricultural Practices and research update'.24th Annual Maryland Organic Food and Farming Association (MOFFA) Winter Meeting February 2015, Annapolis, MD. Sarah Allard, Donna Pahl and Shirley A. Micallef. Presented by S. Allard. 'Microbiological assessment of produce and post-harvest handling methods on small- and medium sized farms in the Mid-Atlantic Region' in Symposium: Trending now: What's new in post-harvest processing of fruits and vegetables,IFT 2014 Annual Meeting,21-24 June, 2014, Presented by S. Pagadala, post-doctoral researcher in Micallef Lab 'Conventional or organic produce? The microbiological question',JIFSAN 2014 Annual Spring Symposium,April 24 - 25, 2014 'Can we reduce risks of fresh produce contamination through improved Good Agricultrual Practices?',Montgomery College Spectrum Lecture Series 2013-2014, April 2014 'GAPs and Microbiological Safety of Fresh Produce',WyeREC Food Safety Training, March 2014, Presentation, Maryland Department of Agriculture and University of Maryland 'GAPs and Organic Production: Microbial Assessment',23rd Annual Maryland Organic Food and Farming Association (MOFFA) Winter Meeting 2014,Annapolis MD Food Safety and Organic Farming,AGNR Convocation,2013 'Safety of Fresh Produce and Farm Studies in the Mid-Atlantic',Good Agricultural Practices for Fruit and Vegetable Producers Training, St. Mary's County, MD, March 2013 - Maryland Department of Agriculture and University of Maryland, Presentation 'Microbiological Quality of Organically Grown Produce in Maryland and Other Mid-Atlantic States',22nd Annual Maryland Organic Food and Farming Association (MOFFA) Winter Meeting 2013,2013, Annapolis MD Poster Presentations Xu A., Pahl D., Buchanan R.L. and Micallef S.A.,Microbiological Assessment and Testing of Organic Pre- and Post-harvest Fresh Produce and Irrigation Water on Maryland Farms for Salmonella, Listeria monocytogenes and Shiga Toxin-producing Escherichia coli,2013 International Association for Food Protection (IAFP) Annual Meeting,2013, Charlotte, NC, Poster Presentation What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? Consumption of locally, organically-grown produce is becoming increasingly popular. In the mid-Atlantic region, farms adopting organic practices typically produce at small production scales, have limited resources and adopt low technology harvesting and post-harvest handling practices. Data on the effect of hand harvesting, field-packing or packing-house handling with minimal treatment on food safety risk at this production scale was lacking. We followed produce from small organic farms in Maryland, from the field through post-harvest handling and packing. Pre- and post-harvest produce and water were analyzed. No pathogens were recovered and no correlation between bacterial levels in irrigation water and on produce were detected. However, post-harvest handling with washing was found to have an impact on leafy greens microbiological status, with higher frequencies of foodborne bacterial indicators post-harvest. Barriers to sanitizer use in leafy greens wash water among small farms using organic practices were revealed. This observation was not noted for dry-packed produce such as tomatoes. In summary, hand harvesting and dry handling did not appear to introduce a significant food safety risk, but washed leafy greens carried higher levels of some microbial indicators, possibly attributed to the lack of sanitizer use in wash water. These findings helped us tailor our presentations and talks to try to bridge thisknowledge gap among farmers to try to motivate behavioural changes. We believe that the development of resources and materials customized for this sector of growers could enhance dissemination of information on best practices for leafy green. Training workshops on Good Agricultural Practices (GAPs) and Good Handling Practices (GHPs) were conducted in 2013 and2014 at the Annual Maryland Organic Food and Farming Association (MOFFA) Winter Meeting. The workshop focussed on GAPs, GHPs pertaining to small organic farming systems, and included discussion on FSMA and the Proposed Produce Safety Rule, as well as the importance of developing a food safety plan.The meeting also allowed additional time to present research updates.

Publications

  • Type: Journal Articles Status: Awaiting Publication Year Published: 2015 Citation: AIXIA XU, DONNA M. PAHL, ROBERT L. BUCHANAN AND SHIRLEY A. MICALLEF*. Comparing the Microbiological Status of Pre- and Postharvest Produce from Small Organic Production. Journal of Food Protection - in press. doi:10.4315/0362-028X.JFP-14-548