Progress 10/01/14 to 09/01/15
Outputs
Target Audience:
Nothing Reported
Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?1. As part of the overall goal to enhance research at Tennessee State University the Evans-Allen grant has enabled several students to participate in research. Students from the Biology and Chemistry Departments in the College of Agriculture, Humans and Natural Sciences (CAHNS) have received significant laboratory training and are able to plan and conduct independent research, and are active participants in both the research phase, and community education/outreach. 2. Specifically, Seyi Falekun, a graduate student in chemistry, who has worked on on how Quercetin, Genistein and Kaempferol do modulate adipocyte cells differentiation in 3T3-L1 preadipocytes. Damitea Johnson continues to generate research data and will be defending her MS degree in the Spring of 2016. They have actively participated in both the experimental and intellectual processes required for the completion of the proposed project. 3. Several undergraduate students worked with the graduate students and received training and credit for their senior research projects. Poster presentations were presented during the Tennessee State University Wide Research Symposium during the grant period and also at national meetings. 4. Rising Seniors, From Martin Luther King (MLK) Magnet School - Two rising senior students from MLK were recruited to undertake research and receive training in my laboratory during the summer of 2015. Such training has helped to prepare them and to conduct research in the STEM discipline as part of the community outreach component of the above research. The said students did conduct research on healthy aspects of the flavonoids and how such compounds could promote healthy eating. How have the results been disseminated to communities of interest?1. Tennessee State University is a Historically Black College/University (HBCU). Student research/support activities facilitated through this project served to help strengthen the skill-sets of the next generation of scientific investigators, and created awareness in healthy eating during presentations of the research findings during the local, regional and national meetings where the research findings were presented. 2. Presentations were made during the Local, Regional and National scientific meetings. In addition research findings in publications have been published in peer-reviewed journals for both the Scientific and general public. 3. The College of Agriculture, Natural and Humans Sciences (CAHNS) holds a Farm Expo during the year. Results of our research were shared with the following: a. Scientific community: Participants who were present during the Farm Expo had the opportunity to visit our poster boards to avail themselves with the results of our research findings. b. Additionally, the participation and presentation of the research findings during the proposed education/outreach demonstration at the Farm Expo brought some members of the Nashville Farmer's Market, located just 2.4 miles from the TSU main campus, and helped educate such individuals on how to help food source producers become more knowledgeable on the potential use of crop products they grow, and serve as a link into new marketing applications. In particular, our future proposed flavonoids beverage formulation process would make an interesting educational topic to present at classes enrolled in the demonstration kitchen located inside the farmer's market. c. For general public: Examining ways to condense the beneficial properties of flavonoids into a more easily consumable produce were shared with the general public present. What do you plan to do during the next reporting period to accomplish the goals?1. To continue to determine how the above-mentioned flavonoids modulate adipocyte differentiation in 3T3-L1 fibroblast cells. There are no reports on investigations into the molecular mechanisms underlying the effects that quercetin and genistein exert on adipogenesis. Thus, the purpose of this objective is to examine the structure-antioxidant-activity relationship to better understand the molecular mechanisms by which these flavonoids may either inhibit or induce apoptosis in the 3T3-Li cell model. 2. The mechanisms of quercetin, genistein and kaempferol induction in both reduced glutathione (GSH) and enzyme activities in glutathione peroxidase, glutathione reductase, superoxide dismutase and glucose-6-phosphate dehydrogenase activities. 3. Continue to train and expose of the youth and other minorities to healthy eating through research and to combat obesity and its related metabolic syndrome thereby reducing morbidity and mortality rates among obese subjects.
Impacts
What was accomplished under these goals?
Focus of research: The proposed hypothesis is that certain plant flavonoids (namely, quercetin, genistein and kaempferol) can prevent oxidative damage, and regular consumption of flavonoid enhanced food products will result in improved health, and beneficial eating habits in obese subjects. Based on the above hypothesis the following were accomplished: (a.) Quercetin, genistein and kaempferol do prevent oxidative damage, thereby lowering the oxidative damage through the metabolic syndrome. (b.) Specifically the studies have shown that the above mentioned flavonoids can enhance the cell's capabilities to better cope with oxidative damage induced by obesity by enhancing the levels of the cell's glutathione (GSH, a natural antioxidant in cells), and its antioxidative through the glutathione peroxidase (GSH-PX), glutathione reductase (GSH-Rx), superoxide dismutase (SOD), and glucose-6-phosphate dehydrogenase (G-6-PDH) pathways. (c.) That the flavonoids did inhibited the differentiation of the 3T3-L1 preadipocytes to adipocyets suggesting the possible molecular mechanisms by which these flavonoids may both inhibit and induce apoptosis in preadipocytes. (d.) That the, above-mentioned flavonoids, can enhance the cell's protective mechanisms through the above-mentioned biochemical parameters and prevent oxidative damage in obese subjects through healthy eating. (e.) Finally, the Evans-Allen funds made available through the NIFA application facilitated the acquisition of the following logistical supplies which are crucial to the implementation of the experiments to be conducted in the above proposed work. These include: • 3T3-LI fibroblast cells • Kits for the analysis of GSH and enzymes • Macromolecule such as hemoglobin and myoglobin • Some cell culture supplies and kits for cell differentiation
Publications
- Type:
Conference Papers and Presentations
Status:
Published
Year Published:
2015
Citation:
1. Boadi W.Y., Amartey P. and Lo A. Relative efficiencies of quercetin, genistein and kaempferol on adipocyte differentiation in 3T3-L1 preadipocytes. (Manuscript in preparation).
2. Boadi W.Y., Amartey P. and Lo A.: Effects of quercetin, genistein and kaempferol on glutathione and glutathione-redox cycle enzymes in 3T3-L1 preadipocyte cells. Drug Chem. Toxicol. Early Online: 1-9, 2015.
3. Boadi W.Y. and Lo A.: Quercetin and kaempferol but not glutathione inhibit lysine, arginine and increase cysteine residues in hemoglobin and myoglobin. J. Free Rad. Antiox. 141:386-398, 2014.
4. Boadi, W. Y., and Johnson, D.: Effects of low doses of quercetin and genistein on oxidation and carbonylation in hemoglobin and myoglobin. J. Diet. Suppl.11(3):272-287, 2014.
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Progress 01/01/13 to 09/01/15
Outputs
Target Audience: Youth especially between the ages of 13-20. The research activities provided valuable information to familiarize source producers on the potential use and application(s) of flavonoids enriched products in prevention-based health marketing.food product research and develop experience for minority students, and help Strengthen the prevention-based health advocacy and outreach within the disadvantaged communities near and around TSU's campus. Provide demonstrations/education to the general public on the health benefits gained from higher intake of flavonoids rich foods and beverages, with particular focus on customers and food stand sellers and Nashville's local farmers' market. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided? As part of the overall goal to enhance research at Tennessee State University the Evans-Allen grant has enabled several students to participate in research. Students from the Biology and Chemistry Departments in the College of Agriculture, Humans and Natural Sciences (CAHNS) have received significant laboratory training and are able to plan and conduct independent research, and are active participants in both the research phase, and community education/outreach. Specifically, Seyi Falekun, a graduate student in chemistry, who has worked on on how Quercetin, Genistein and Kaempferol do modulate adipocyte cells differentiation in 3T3-L1 preadipocytes. Damitea Johnson continues to generate research data and will be defending her MS degree in the Spring of 2016. They have actively participated in both the experimental and intellectual processes required for the completion of the proposed project. Several undergraduate students worked with the graduate students and received training and credit for their senior research projects. Poster presentations were presented during the Tennessee State University Wide Research Symposium during the grant period and also at national meetings. Rising Seniors, From Martin Luther King (MLK) Magnet School - Two rising senior students from MLK were recruited to undertake research and receive training in my laboratory during the summer of 2015. Such training has helped to prepare them and to conduct research in the STEM discipline as part of the community outreach component of the above research. The said students did conduct research on healthy aspects of the flavonoids and how such compounds could promote healthy eating. How have the results been disseminated to communities of interest? Tennessee State University is a Historically Black College/University (HBCU). Student research/support activities facilitated through this project served to help strengthen the skill-sets of the next generation of scientific investigators, and created awareness in healthy eating during presentations of the research findings during the local, regional and national meetings where the research findings were presented. Presentations were made during the Local, Regional and National scientific meetings. In addition research findings in publications have been published in peer-reviewed journals for both the Scientific and general public. The College of Agriculture, Natural and Humans Sciences (CAHNS) holds a Farm Expo during the year. Results of our research were shared with the following: Scientific community: Participants who were present during the Farm Expo had the opportunity to visit our poster boards to avail themselves with the results of our research findings. Additionally, the participation and presentation of the research findings during the proposed education/outreach demonstration at the Farm Expo brought some members of the Nashville Farmer's Market, located just 2.4 miles from the TSU main campus, and helped educate such individuals on how to help food source producers become more knowledgeable on the potential use of crop products they grow, and serve as a link into new marketing applications. In particular, our future proposed flavonoids beverage formulation process would make an interesting educational topic to present at classes enrolled in the demonstration kitchen located inside the farmer's market. For general public: Examining ways to condense the beneficial properties of flavonoids into a more easily consumable produce were shared with the general public present enrolled in the demonstration kitchen located inside the farmer's market. What do you plan to do during the next reporting period to accomplish the goals?
Nothing Reported
Impacts
What was accomplished under these goals?
Based on the above hypothesis the following were accomplished: Quercetin, genistein and kaempferol do prevent oxidative damage, thereby lowering the oxidative damage through the metabolic syndrome. Specifically the studies have shown that the above mentioned flavonoids can enhance the cell's capabilities to better cope with oxidative damage induced by obesity by enhancing the levels of the cell's glutathione (GSH, a natural antioxidant in cells), and its antioxidative through the glutathione peroxidase (GSH-PX), glutathione reductase (GSH-Rx), superoxide dismutase (SOD), and glucose-6-phosphate dehydrogenase (G-6-PDH) pathways. That the flavonoids did inhibited the differentiation of the 3T3-L1 preadipocytes to adipocyets suggesting the possible molecular mechanisms by which these flavonoids may both inhibit and induce apoptosis in preadipocytes. That the, above-mentioned flavonoids, can enhance the cell's protective mechanisms through the above-mentioned biochemical parameters and prevent oxidative damage in obese subjects through healthy eating. Finally, the Evans-Allen funds made available through the NIFA applicationfacilitated the acquisition of the following logistical supplies which are crucial to the implementation of the experiments to be conducted in the above proposed work. These include: 3T3-LI fibroblast cells Kits for the analysis of GSH and enzymes Macromolecule such as hemoglobin and myoglobin Some cell culture supplies and kits for cell differentiation
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2015
Citation:
2. Boadi W.Y., Amartey P. and Lo A.: Effects of quercetin, genistein and kaempferol on glutathione and glutathione-redox cycle enzymes in 3T3-L1 preadipocyte cells. Drug Chem. Toxicol. Early Online: 1-9, 2015.
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Progress 10/01/13 to 09/30/14
Outputs
Target Audience:
Nothing Reported
Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?
Nothing Reported
How have the results been disseminated to communities of interest?
Nothing Reported
What do you plan to do during the next reporting period to accomplish the goals? Pending Experiments Specific Aim 2 “Determine the relative efficiencies of quercetin, genistein and kaempferol on adipocyte differentiation in 3T3-L1 fibroblast cells” There are no reports on investigations into the molecular mechanisms underlying the effects that quercetin and genistein exert on adopogenesis. Thus, the purpose of this objective is to examine the structure-antioxidant-activity relationship to better understand the molecular mechanisms by which these flavonoids may either inhibit and induce apoptosis in the 3T3-Li cell model.
Impacts
What was accomplished under these goals?
. Experiments & Accomplishments Experiments have been conducted throughout the academic year on specific aim 1 i.e., “Determine the relative efficiencies of three abundant flavonoids (i.e., quercetin, genistein and kaempferol in reducing oxidative stress through the glutathione (GSH) and GSH-Redox pathways using mouse preadipocytes 3T3-L1 fibroblast cells”. The effort has resulted in the manuscript below which has been submitted tom the journal of Drug and Chemical Toxicology for consideration and publication.
Publications
- Type:
Other
Status:
Under Review
Year Published:
2014
Citation:
Boadi W.Y., Amartey P. and Lo A.: Effects of quercetin, genistein and kaempferol on glutathione and glutathione-redox cycle enzymes in 3T3-L1 preadepocyte cells. Manuscript submitted to Drug and Chemical Toxicology (2014).
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Progress 01/01/13 to 09/30/13
Outputs
Target Audience:
Nothing Reported
Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided? 3. Output An abstract will be generated based on the preliminary data after some of the experiments in specific aim 1 have been completed and will be submitted for consideration and publication in future scientific meetings. How have the results been disseminated to communities of interest?
Nothing Reported
What do you plan to do during the next reporting period to accomplish the goals? Focus of research: We intend to carry studies on the hypothesis that certain plant flavonoids (namely, quercetin, genistein and kaempferol) can reduce oxidative stress through the GSH and GSH-Redox pathways using mouse preadipocytes 3T3-L1 fibroblasts cells. Secondly, how the above mentioned flavonoids affect adipocytes differentiation in 3T3-L1 cells in vitro.The studies seeks to address how the flavonoids can be recommended to improve the diets in obese subjects.
Impacts
What was accomplished under these goals?
2. Experiments Experiments have been conducted to establish the effect of the above flavonoids on 3T3-LI fibroblast cell growth and viability. These preliminary studies were necessary for establish the dose levels needed to be used in addressing specific aims 1 and 2. Experiments are also currently being planned to address the experiments to be conducted in specific aim 1.
Publications
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