Source: CORNELL UNIVERSITY submitted to NRP
COARSE WOODY DEBRIS AS A SOURCE OF STABILIZED SOIL ORGANIC MATTER IN TEMPERATE FOREST
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
0231365
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
Oct 1, 2012
Project End Date
Sep 30, 2015
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
CORNELL UNIVERSITY
(N/A)
ITHACA,NY 14853
Performing Department
Natural Resources
Non Technical Summary
Soil organic matter (SOM) plays a variety of important roles in forest ecosystems, storing carbon, maintaining fertility and promoting favorable structure and porosity of soil. Organic carbon in soils is a major pool in the global C cycle, and potential changes in this pool could serve as either a significant sink or source for atmospheric carbon dioxide. Management options for promoting the C sink strength in soil have been proposed including forest management activities; however, fundamental understanding of the dynamics of forest SOM is incomplete. The principal justification for this proposed research is to improve understanding of the sources of forest SOM so that society has a better basis for judging the effects of forest management activities on this crucial ecosystem component. The proposed work is particularly timely as a result of recent and likely future developments in energy and carbon markets. Among the variety of schemes to reduce the carbon footprint of human activity are two that the proposed research would inform: woody biofuels and forest carbon offset markets. In both cases inadequate understanding of the source and fates of forest SOM constrain our capacity to promote an optimal pathway forward. By improving understanding of the contribution of woody biomass to SOM stabilization, this research will facilitate mechanistic modeling of these processes. The key society issue that the proposed research will address is the interaction between land use and global climate change. Society can help incentivize forest management activities that will reduce our C footprint but only if basic information on soil C dynamics is available to inform policymakers. By quantifying the sources of stabilized SOM in forests the proposed research will contribute to this pressing issue.
Animal Health Component
(N/A)
Research Effort Categories
Basic
(N/A)
Applied
(N/A)
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
12306201070100%
Goals / Objectives
The overall goal of the proposed study is to improve understanding of the sources of stabilized soil organic matter (SOM) in temperate deciduous forest ecosystems. Four specific objectives for the project are: 1. To quantitatively trace carbon from decomposing woody detritus into stabilized SOM pools including different aggregate fractions. 2. To examine the differences in stabilization in soil of C from woody detritus between wood inoculated with brown-rot vs. white-rot fungi. 3. To quantitatively trace carbon from decomposing tree root systems into stabilized SOM pools. 4. To quantify the fate of C in ephemeral mycorrhizal fine roots in soil horizons of northern hardwood forest ecosystems. The principal outcome of the proposed research will be estimates of the proportional contribution to stabilized soil C of wood, leaf litter, and fine and coarse roots in northern hardwood forest ecosystems. Based on limited information in the literature we expect that a higher proportion of stabilized SOM will be derived from roots rather than aboveground woody and non-woody detritus. These results will inform improved models of the impacts of forest management activities on long-term soil C sequestration.
Project Methods
The overall approach to be employed in the proposed project is stable isotope tracing using plant tissue highly-enriched in 13C. The labeled material for the study was produced by fumigating sugar maple trees in a northern hardwood forest with 45 percent atom-enriched 13CO2. The procedure was successful in enriching sugar maple leaves, wood and roots to over 200 per mil del 13C, sufficient to successfully trace 13C from decaying leaf litter into the large soil C pool. For the proposed study wood and root tissues will be harvested and utilized to trace detrital 13C into soil pools. For objective 1, we will harvest all the trees from four 13C fumigation chambers, incubate them on the soil surface in a northern hardwood forest and periodically collect subsamples of wood and underlying soil for analysis of 13C. For Objective 2, in conjunction with the main experiment, we will inoculate 13C labeled wood sections with either common brown-rot or white-rot fungi of hardwoods. A third set of wood samples will be uninoculated controls. Samples of decaying wood and the soil underlying the quadrats will be collected after 2, 3 and 5 years of incubation in the field site at Arnot Forest, NY. Treatment (fungal group) effects on wood decay and 13C recovery will be evaluated using ANOVA for repeated measures. For Objective 3, we will quantify delta 13C of soil in the chambers where the maple trees were originally labeled and will be harvested in fall 2012, the latter marking time zero for calculations of stabilization of root system C in SOM. The coring and analysis procedure will be repeated on the same time schedule as for Objectives 1 and 2 (i.e. years 2, 3 and 5, adjusted as necessary). The basis of statistical inference for the root decay study will be the four replicate labeling chambers. For Objective 4, we will quantify the stabilization of C in fine roots collected from the chambers in fall 2011, one year after initial labeling (when delta 13C in fine roots was at a maximum). Root samples will be mixed with mineral soil at a level corresponding to root length density observed in soils at our field site. Random sub-sets of five cores will be collected after 0.5, 1, 1.5 and 2 years. Comparisons of C stabilization between the two classes of fine roots will be conducted using one-way ANOVA.

Progress 10/01/12 to 09/30/15

Outputs
Target Audience:The target audiences for this research include forest scientists and their graduate students; forest research agencies (e.g., USFS, NYS DEC); forest managers; forest policymakers; and non-governmental organizations involved in climate policy. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?During the course of this research we provided training for technicians and undergraduate students. This included training in chemical methods for preparation of samples for isotope analysis and for separating aggregate fractions in soil. The development of an Honors Thesis of an undergraduate student was conducted through this work. The student learned about experimental design, statistical analysis and principles underlying carbon sequestration in forests. How have the results been disseminated to communities of interest?The results of this research are not yet sufficiently developed to warrant dissemination to target audiences. What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? Our experiment to quantify sources of soil carbon was established at field sites in NYS. Roots and branches were successfully labeled with a heavy stable isotope of C. These substrates were deployed in sugar maple forests, and background C isotope abundance was measured in the forest soils. Woody substrates were inoculated with either brown or white rot fungi. Statistical analysis of natural isotope abundance in soils and isotope enrichment in labeled substrates confirmed that we will readily be able to detect the contribution of these substrates to soil organic matter pools as substrate decay proceeds over coming years. We completed two collections of fast decaying fine root samples after one and two years of decay. The light fraction of soil organic matter was highly enriched in fine root-derived C and analysis of five other soil aggregate fractions is in progress. We also completed the first collection of slower decaying woody substrates in 2015 and these samples are being processed for isotope analysis. Collection of fine root samples is scheduled for October 2016 and 2017, and collection of woody substrates for October 2017 and 2019. After completion of isotope analyses on these samples we expect to be able to quantify the proportional contribution of each of these substrates to stabilized soil organic matter. We will then provide recommendations to forest policy and management agencies and professionals on the prospects for sequestering atmospheric C in soil organic matter of managed and unmanaged forests.

Publications


    Progress 10/01/13 to 09/30/14

    Outputs
    Target Audience: Nothing Reported Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? An undergraduate student is pursuing a Senior Honors Research Thesis based on experiment 2, described above. How have the results been disseminated to communities of interest? Nothing Reported What do you plan to do during the next reporting period to accomplish the goals? During the coming year we will be processing samples for two experiments. Analysis of carbon isotope ratios in coarse woody debris samples will be conducted. Processing of soil cores for aggregates and carbon isotope ratios will be completed. We will also collect the second set of soilsamples for experiment 3 and process these samples for aggregates and carbon isotope ratios. Data from the three experiments will be organized and analyzed to guide planning of future collections.

    Impacts
    What was accomplished under these goals? During 2014 we continued three experiments in pursuit of project goals. For experiment 1 we collected samples of incubating coarse woody debris, and we are currently processing these samples for carbon isotope analysis. For experiment 2 we collected a set of fine root soil cores. The cores were processed for soil aggregates and carbon isotopes. For experiment 3 we completed aggregate separations and initial carbon isotope ratio analysis.

    Publications


      Progress 10/01/12 to 09/30/13

      Outputs
      Target Audience: Nothing Reported Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? Two undergraduate students and one technician have received field and laboratory training on the project. They have learned about sample processing for isotope analysis and soil aggregate fractionation. The technician has received training in the identification and handling of fungal inoculum. Students and technician have also received training in experimental design for field studies. How have the results been disseminated to communities of interest? Nothing Reported What do you plan to do during the next reporting period to accomplish the goals? During the coming year we will collect samples from field sites for the three experiments.. 1. Decaying woody detritus will be collected in fall 2014, together with underlying soil, for analysis of isotope recovery. 2. Incubation tubes containing labeled fine roots will be collected in spring 2014, and the recovery of isotopes in soil aggregate fractions will be quantified. 3. Soil pits will be excavated in the labeling chambers in fall 2014, following the same procedure as for time zero collections. Samples will be processed to quantify isotope recovery in soil aggregate reactions. Data from all three experiments will be organized, analyzede and used to schedule future field collections.

      Impacts
      What was accomplished under these goals? During 2013 three experiments were initiated in pursuit of project goals. 1. Isotopically labeled woody detritus was prepared for field incubations. These samples were inoculated with fungi and placed in field sites. Time zero subsamples are being analyzed. 2. Isotopically labeled fine roots were collected and mixed with soil in incubation tubes. These samples were deployed at the field site. Time zero subsamples are being analyzed. 3. Soil samples were collected from field labeling chambers. These samples are being processed as time zero controls for quantifying the recovery of isotope label in soil aggregate fractions. Data management of time zero samples is underway.

      Publications