NATIONAL ANIMAL GENOME RESEARCH PROGRAM

• Sponsoring Institution: National Institute of Food and Agriculture
• Project Status: COMPLETE
• Funding Source: HATCH
• Reporting Frequency: Annual
• Accession No.: 0231109
• Multistate No.: NRSP-_OLD8
• Project Start Date: Oct 1, 2012
• Project End Date: Sep 30, 2013
• Program Code: [(N/A)]- (N/A)

Recipient Organization
COLORADO STATE UNIVERSITY
(N/A)
FORT COLLINS,CO 80523

Performing Department
Animal Science

Non Technical Summary
Various physiological events are required for successful and efficient livestock production. Reproductive failures and growth and development performance are among the most important. Identification of genes and gene expression related to these physiologic events is critical. These series of experiments will first identify those unique traits then work to associate them in animal production.

Animal Health Component

(N/A)

Research Effort Categories

Basic

(N/A)

Applied

(N/A)

Developmental

(N/A)

Classification

Knowledge Area (KA)	Subject of Investigation (SOI)	Field of Science (FOS)	Percent
301	3810	1020	50%
303	3810	1020	10%
304	3810	1020	20%
305	3810	1020	20%

Knowledge Area
303 - Genetic Improvement of Animals; 305 - Animal Physiological Processes; 304 - Animal Genome; 301 - Reproductive Performance of Animals;

Subject Of Investigation
3810 - Horses, ponies, and mules;

Field Of Science
1020 - Physiology;

Keywords

transcriptome

reproduction

performance

Goals / Objectives
Create shared genomic tools and reagents and sequence information to enhance the understanding and discovery of genetic mechanisms affecting traits of interest. Facilitate the development and sharing of animal populations and the collection and analysis of new, unique and interesting phenotypes. Develop, integrate and implement bioinformatics resources to support the discovery of genetic mechanisms that underlie traits of interest.

Project Methods
1. Tissue samples have and will continue to be collected from animals of interest, namely equine reproductive (Bruemmer) and bovine performance (Thomas). 2. RNA will will be extracted and submitted for deep sequencing. 3. Data will be analyzed and unique transcripts related to traits of interest identified. Findings and collaborations will be discussed at annual meeting and disseminated through scientific journal publication.

Progress 10/01/12 to 09/30/13

Outputs
Target Audience: Horse breeders and reproductive physiologists Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? Increased opportunity for graduate and undergraduate training How have the results been disseminated to communities of interest? Published in refereed journals and presented at professional society and interest group meetings What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? 1. Published transcriptome related to prostaglandin transporters related to pregnancy staus of the mare. 2. Attended and participated in PAG meeting

Publications

• Type: Journal Articles Status: Published Year Published: 2012 Citation: Patterson AL, Bouma JG, Squires EL, Hansen TR, Bruemmer JE. Transcriptional profile of the day 18 endometria from pregnant and non-pregnant mares: Insight into maternal recognition of pregnancy. Mol Reprod Dev 2012 79:777-796. Daigneault BW, Graham JK, Bruemmer JE, Denniston DD, Carnevale EM. Cryopreservation of Cooled Semen and Evaluation of Sperm Holding Media for Potential Use in Equine-Assisted Reproduction Procedures, Journal of Equine Veterinary Science. 2012 32:596-574. Magee C, Bruemmer JE, Nett TM, Squires EL, Clay CM. Kisspeptide in the estrous mare: is it an appropriate ovulation-inducing agent? 2012. Theriogenology 78:1987-1996.
• Type: Conference Papers and Presentations Status: Accepted Year Published: 2012 Citation: Hergenreder JR, da Silveira JC, Belk AD, Veeramachaneni DNR, Bouma JG, Bruemmer JE Serum exosome profile as related to early pregnancy staus in the mare. 2012 Amer Soc Anim Sci Anual Mtg Phoenix AZ July 16, 2012 Cleys ER, Halleran JL, da Silveira J, Winger QA, Bruemmer JE, Clay CM, Bouma GJ. Prenatal androgenization decreases global methylation in placental tissue and increases placental ESR2, DNMT3, DNMT3b and H19 mRNA in sheep. 2012 Society for Study of Reproduction Annual Meeting, State College PA. August 10-13, 2012 Cameron A, Hergenreder J, da Silveira J, Belk A, Klohanatz K, Veeramachaneni, Bouma GJ, Bruemmer JE. Exosomal miRNAs as biological markers of pregnancy status in the mare. 2012 Society for Study of Reproduction Annual Meeting, State College PA. August 10-13, 2012 Klohanatz K, Bouma GJ, Bruemmer JE. Equine endometrial gene expression during maternal recognition of pregnancy. 2012 Rocky Mountain Reproductive Sciences Symposium Loveland Co April 21, 2012 Cleys ER, Halleran JL, da Silveira J, Winger QA, Bruemmer JE, Clay CM, Bouma GJ. Effects of prenatal androgenization on the sheep placentome. 2012 Rocky Mountain Reproductive Sciences Symposium Loveland Co April 21, 2012. Cameron A, Hergenreder J, da Silveira J, Belk A, Klohanatz K, Veeramachaneni DNR, Bouma GJ, Bruemmer JE. Exosomal miRNAs as biological markers of successful maternal recognition of pregnancy in the mare. 2012 Rocky Mountain Reproductive Sciences Symposium Loveland Co April 21, 2012.