Progress 10/01/12 to 09/30/17
Outputs
Target Audience:The target audience for our journal articles was the scientific community in entomology, plant pathology, and plant biology, and the target audience for our technical reports were the Arkansas soybean growers. Our outreach activities reached local teachers and parents as well as the K-12 audience, and our other educational efforts servicedundergraduate and postgraduate student populations. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?This project has provided training opportunities for one laboratory technician, 3 postdoctoral associates, 2 Ph.D. students, and 1 undergraduate (1 hourly research assistant). Bothgraduate students and 1 postdocotral fellowpresented their work at at least one scientific conference. One Ph.D. student also had the opportunity to serve as president of the Arkansas Enotomological Society and organize their annualconference and outreach event. This student also received an honorable mention in the NSF graduate fellowship competition, and was awarded second place in the Entomological Society of America's Ph.D. presentation competition. How have the results been disseminated to communities of interest?This year we have published two peer-reviewed scientific papers, 1 book chapter,and 1 technical report based on this work, and we have one additional publication in review, and two in preparation. We have also presented this work as part of seminar series at other universities, and at a variety of scientific meetings including the Annual Meeting of the Entomological Society of America. What do you plan to do during the next reporting period to accomplish the goals?
Nothing Reported
Impacts
What was accomplished under these goals?
In 2017, my laboratory examined plant redox responses to aphid infestation and utilized a conditional mutation to manipulate ROS accumulation in the host plant. Our results indicate that aphid infestation induces an oxidative response in multiple subcellular compartments in the host plant, including the chloroplast, and that ROS accumulation in the chloroplast can enhance aphid resistance in Arabidopsis. This result to our knowledge has never been reported before, and dramatically advances our understanding of defensive signaling in response to aphids. We have, through virus-induced gene silencing, also identified a putative resistance gene (R gene) and a gene involved in fatty acid metabolism that contribute to aphid resistance in tomato. Furthermore, we have successfully utilized the CRISPR-Cas9 system to develop new mutant lines in tomato and Arabidopsis that are useful for studying aphid resistance and other forms of plant defense. In Arabidopsis, we have developed lines with multiple defects in fatty acid desaturation in order to pinpoint the effects of specific fatty acids on aphid resistance. In tomato, we have developed stable lines carrying null mutations in Non-expressor of Pathogenesis-Related Protein 1 (NPR1), a redox-regulated transcriptional activator that mediates the majority of transcriptional responses to the defensive hormone salicylic acid. NPR1 is not only required certain forms of aphid resistance in tomato, but is also critical to many forms of pathogen resistance, and the lines we have developed represent a valuable resource not only for studying aphid resistance, but also for the larger plant immunity community.
Publications
- Type:
Book Chapters
Status:
Published
Year Published:
2017
Citation:
Goggin F.L., Quisenberry S.S., and Ni X. 2017. Chapter 14: Feeding Injury. Invited chapter in Aphids as Crop Pests, 2nd ed., eds H.M. van Emden and R. Harrington, CABI, Oxfordshire, UK.
- Type:
Journal Articles
Status:
Published
Year Published:
2017
Citation:
Xu J., Tran T., Padilla C., Braun D., and Goggin F.L. 2017. Superoxide-responsive genes expression in Arabidopsis thaliana and Zea mays. Plant Physiology and Biochemistry 117, 51-60.
- Type:
Journal Articles
Status:
Published
Year Published:
2017
Citation:
Lee M., Huffaker A., Crippen D., Robbins R.T., and Goggin F.L. 2017. Plant Elicitor Peptides Promote Plant Defenses against Nematodes in Soybean. Molecular Plant Pathology, DOI: 10.1111/mpp.12570, http://onlinelibrary.wiley.com/doi/10.1111/mpp.12570/abstract?campaign=wolacceptedarticle.
- Type:
Journal Articles
Status:
Published
Year Published:
2017
Citation:
" Lee, M.1, Huffaker, A., and Goggin, F.L. 2017. Use of Plant Elicitor Peptides as Signaling Molecules to Induce Nematode Resistance in Soybean. Arkansas Soybean Research Series, Arkansas Experiment Station Research Series 631.
- Type:
Journal Articles
Status:
Under Review
Year Published:
2018
Citation:
Li, J. , Avila, C.A., Tieman, D.M., Klee, H.J., and Goggin, F.L. A Comparison of the Effects of Fatty Acid Desaturase 7 and Hydroperoxide Lyase on Plant-Aphid Interactions.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Fischer, H.D. and Goggin, F.L. Elucidating the mechanism of aphid resistance in Fatty Acid Desaturase 7 Arabidopsis mutants. Entomological Society of America Annual Meeting, Denver, CO. (oral presentation). Won 2nd Place in PhD presentation competition.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Wickramanayake J.S. and F.L. Goggin. 2017. Influence of fatty acid desaturation on photosynthetic parameters and aphid resistance in tomato. Entomological Society of America Annual Meeting, Denver, CO. (oral presentation).
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2017
Citation:
Wickramanayake J.S. and F.L. Goggin. 2017. Influence of fatty acid desaturation on photosynthetic parameters and aphid resistance in tomato. Oklahoma State University Department of Entomology and Plant Pathology Seminar Series, Stillwater, OK. (invited seminar; JW was the speaker).
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Progress 10/01/15 to 09/30/16
Outputs
Target Audience:The target audience for our publications was the scientific community, and the target audience for our educational activities was the K through postgraduate student population, as well as the general public. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?This project has provided training opportunities for one laboratory technician, 4postdoctoral associates, 3 Ph.D. students, and 1 undergraduate(1 hourly research assistant). One Ph.D. student graduated this year and completed a dissertationon herwork. All postdoctoral students and 1 graduate student each presented their work at at least one scientific conference. I also developed a professional development workshop on interview skills (including an invited speaker from the plant biotechnology industry) that was attended by most trainees in the lab. All lab members also participated in K12 outreach activities to gain experience with teaching. As a result of theseoutreach activities, 10 Arkansas teachers also received training in a classroom module on plant biology and genetics.These teachers participated ina summer workshop we offered at the Arkansas School for Mathematics, Science, and Arts, where we provided all the materials and training needed to engage students ina semester-long citizen science experiment. How have the results been disseminated to communities of interest?We have submitted one manuscript and 1 report based on this work, and are preparing two additional publications for submission. We have also presented this work as part of seminar series at other universities, and at a variety of scientific meetings including the International Congress of Entomology and the triennial meeting of the International Society of Molecular Plant Microbe Interactions. What do you plan to do during the next reporting period to accomplish the goals?In the coming year, we will continue to investigate the genetic and biochemical basis of plant defenses against aphids and nematodes, and the collateral impacts of defense on other agriculturally important traits, like growth, development, and nutritional content.
Impacts
What was accomplished under these goals?
In 2016, my laboratory examined plant redox responses to aphid infestation using redox-sensitive probes or reporter genes responsive to diffrent reactive oxygen species (ROS). Our results indicate that aphids induce oxidative responses in their hostsand appear to enhance accumulation of singlet oxygen, a highly reactive ROS generated in the chloroplast. Work is currently underway to investigate the influence of this oxidative response on plant defenses against aphids. We havealso generated double mutant lines of Arabidopsis thaliana and begun development of a CRISPR mutant in tomato to investigate the contribution of aredox-regulated transcriptional activator (NPR1)to aphid resistance in thesespecies. This year, my laboratory has also conducted research on the role of plant elicitor peptides (Peps) in plant defense signaling. Preliminary experimentsdid not detect an effect of Peps on plant-aphid interactions, but established that Peps can activate plant defenses against another class of herbivores that feed from the plant vasculature, phytoparasitic nematodes. We determined that three Peps from soybean could enhance resistance to root-knot nematodes (Meloidogyne incognita) and soybean cyst nematodes (Heterodera glycines) when in vitro-synthesized Peps were applied asseed treatments. Peps appeared to immunize plants against nematode infection by increasing ROS accumulation and activating expression of defense genes, including NADPH oxidase D. Together, these projects have enhanced our understanding of plant defensive responses against vascular herbivores, generated useful new genetic materials for the plant science community, and identified seed treatments with potential utility for nematode management.
Publications
- Type:
Journal Articles
Status:
Submitted
Year Published:
2017
Citation:
Lee, M.W., Huffaker, A., Crippen, D.,Robbins, R., and Goggin, F.L. Plant Elicitor Peptides Promote Plant Defenses against Nematodes in Soybean. Submitted to Molecular Plant Pathology; in review.
- Type:
Journal Articles
Status:
Submitted
Year Published:
2017
Citation:
Lee, M.W., Huffaker, A., and Goggin, F.L. Use of Plant Elicitor Peptides as Signaling Molecules to Induce Nematode Resistance in Soybean. Soybean Research Series,Arkansas Division of Agriculture Communications.
- Type:
Theses/Dissertations
Status:
Published
Year Published:
2016
Citation:
Li, J. 2016. Influence of Fatty Acids and their Derivatives on Aphid Resistance in Arabidopsis and Tomato. Ph.D. dissertation, University of Arkansas, Fayetteville, AR.
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Progress 10/01/14 to 09/30/15
Outputs
Target Audience:The target audience for our publications was the scientific community, and the target audience for our educational activities was the Kthrough postgraduate student population. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?This project has provided training opportunities foronelaboratory technician, 3postdoctoral associates, 1M.S. and 2 Ph.D. students, and three undergraduates (1 hourly research assistantand 1honors student). One undergraduate andone MS student graduated this year and completed theses on their work. How have the results been disseminated to communities of interest?We have published high-ranking peer-reviewed journal articlesand been invited to present our work at scientific forums at the national level. What do you plan to do during the next reporting period to accomplish the goals?In the coming year, we will continue to investigate the genetic and biochemical basis of plant defenses against aphids and nematodes, and the collateral impacts of defense on other agriculturally important traits, like growth, development, and nutritional content.
Impacts
What was accomplished under these goals?
In 2015, my laboratorydeveloped newgenetic materials to study the influence of plant fatty acid profiles on redox responses to insects and other stresses. We did this by transferring a suiteof redox-responsive reporter genes into Arabidopsis genotypes with normal and modified fatty acid profiles. These reporter genes can be used to visualizewhen and where hydrogen peroxide and singlet oxygen accumulate in the plant, and also to assess the overall redo status of the cytosol and chloroplast. My laboratory has begun to use these materials to compare redox signaling in plantswith normalfatty acid profiles, and mutants with reduced levels of fatty acid desaturation. Our preliminary results suggest that reduced desaturation promotes accumulation of reactive oxygen species, and this may contribute to the enhanced aphid resistance observed in mutants with modified fatty acid profiles. This work sheds light on mechanisms of insect resistance in plants, and has also generated genetic tools that should be useful to the plant biology community.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2015
Citation:
Goggin, F.L., Lorence, A., and Topp, C.N. 2015. Applying high-throughput phenotyping to plant-insect interactions: picturing more resistant crops. Current Opinion in Insect Science, 9, 69-76.
- Type:
Journal Articles
Status:
Published
Year Published:
2015
Citation:
Goggin, F.L., Zhu-Salzman, K. 2015. Pests and resistance: Social networking---studying the web of plant-insect interactions to improve host plant resistance. Current Opinion in Insect Science, 9, 69-76.
- Type:
Journal Articles
Status:
Published
Year Published:
2015
Citation:
Avila, C.A., Wu, C., and Goggin, F.L. 2015. The ethylene response factor Pti5 contributes to potato aphid resistance in tomato independent of ethylene signaling. Journal of Experimental Biology, 66: 559-570.
- Type:
Journal Articles
Status:
Published
Year Published:
2015
Citation:
Pallipparambil, G.R., Sayler, R.J., Shapiro, J.P., Jean M.G. Thomas, J.M.G., Kring, T.J., and Goggin, F.L. 2015. Mi-1.2, an R gene for aphid resistance in tomato, has direct negative effects on a zoophytophagous biocontrol agent,Orius insidiosus, Journal of Experimental Botany, 66: 549-557.
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Progress 10/01/13 to 09/30/14
Outputs
Target Audience: The target audience for our publications was the scientific community, and the target audience for our educational activities was the Kthrough postgraduate student population. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided? This project has provided training opportunities to one half-time laboratory technician, 2 postdoctoral associates, 2 M.S. and 2 Ph.D. students, and three undergraduates (1 summer intern and 2 honors student projects). One undergraduate, one PhD., and two postdocs authored publications, and both honors students also applied for and received competitive summer internship support. How have the results been disseminated to communities of interest? We have published high-ranking peer-reviewed journal articles, and been invited to present our work at scientific folrums at the national and international level. What do you plan to do during the next reporting period to accomplish the goals? In the coming year, we will continue to investigate the genetic and biochemical basis of plant defenses against aphids and nematodes, and the collateral impacts of defense on other agriculturally important traits, like growth, development, and nutritional content.
Impacts
What was accomplished under these goals?
This year we established a gene that contributes to pathogen resistance in tomato also contributes to plant defenses against aphids (Avila et al., in press). This works gives us a better understanding of the molecular basis of aphid resistance, as well as the overlap in plant responses to different sources of stress. We also demonstrated that aphid-resistant cultivars can in some cases have direct negative effects on beneficial predatory insects that carry out supplemental feeding on plants (Palliparambil et al., 2014). These results have important implications for integrated pest management, and suggest that there are tradeoffs between host plant resistance and biological control as management strategies.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2014
Citation:
Pallipparambil, G.R., Sayler, R.J., Shapiro, J.P., Jean M.G. Thomas, J.M.G., Kring, T.J., and Goggin, F.L. 2014. Mi-1.2, an R gene for aphid resistance in tomato, has direct negative effects on a zoophytophagous biocontrol agent, Orius insidiosus, Journal of Experimental Botany, doi:10.1093/jxb/eru361. Open Access available at http://jxb.oxfordjournals.org/content/early/2014/09/04/jxb.eru361.full.pdf+html?sid=1f2d1e15-e731-4494-bf7d-30470f10e53c
- Type:
Journal Articles
Status:
Submitted
Year Published:
2014
Citation:
Avila, C.A. , Wu, C. , and Goggin, F.L. In press. The ethylene response factor Pti5 contributes to potato aphid resistance in tomato independent of ethylene signaling. Journal of Experimental Botany.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2014
Citation:
Goggin, F.L. 2014. Molecular determinants of host plant resistance to aphids: impacts of fatty acid metabolism. Invited oral presentation, Annual Meeting of the Society of Experimental Biology, Manchester, UK, July, 2014.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2014
Citation:
Goggin, F.L. 2014. Molecular biology, physiology, and ecological impacts of host plant resistance to aphids. Invited oral presentation, Entomology Department seminar series, Penn State University, State College, PA, April, 2014.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2014
Citation:
Goggin, F.L. 2014.Deciphering host plant defenses against aphids and their ecological impacts. Invited oral presentation, Seminar series, James Hutton Institute, Dundee, UK, July, 2014.
- Type:
Journal Articles
Status:
Published
Year Published:
2014
Citation:
Vaughn, K., Avila, C.A. , Padilla, C. , and Goggin, F.L. 2014. Development of fad7-1 single mutant Arabidopsis thaliana plants that are resistant to aphids. Discovery 15: 94-99. (the UA College of Agricultures student research journal).
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Progress 10/01/12 to 09/30/13
Outputs
Target Audience: The results of our efforts are communicated to the scientific community through peer-reviewed publications and scientific presentations. The project also advances the education of undergraduate, graduate, and postdoctoral trainees through hands-on learning opportunities, and we engage primary school students, teachers, and the general public through outreach events. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided? This project has provided training opportunities for two postdoctoral associates (both under-represented minorities, 1 male, 1 female), 2 Ph.D. students (female), and three undergraduates (2 female, 1 male). All of these trainees have participated in conducting experiments, and 4 of the participants have also gained experience in grant-writing. One postdoctoral associate is a coPI on a research proposal (pending), and 1 undergraduate was recently awarded a SURF research fellowship. One graduate student attended an intensive 6-day workshop in California to learn a critical technique for her research project (the electrical penetration graph technique), and also had the opportunity to visit a collaborating laboratory in California to learn a new cryofixation technique. One postdoctoral associate received training in confocal microscopy, while the other postdoctoral associate attended a proteomics workshop. How have the results been disseminated to communities of interest? We have published our research findings in a high-ranking peer-reviewed scientific journal (Molecular Plant Microbe Interactions), and presented our work at the Annual Meeting of the Entomological Society of America, an invited seminar at the University of Missouri, and at other scientific venues. What do you plan to do during the next reporting period to accomplish the goals? In the coming year, we will continue to investigate transcritomic and metabolomics changes in plants in response to aphid infestation, and will utilize functional genomics to identify genes that contribute to plant defenses against aphids. We will also characterize the accumulation of reactive oxygen species in response to aphids, and their influence on levels of resistance or susceptibility to aphid infestation.
Impacts
What was accomplished under these goals?
This year we established that the alpha-dioxygenase 1 gene contributes to basal defenses against aphids in plants from two different families, tomato (Solanaceae) and Arabidopsis (Brassicaceae). This suggests that the role of this gene in limiting insect infestations is probably widely conserved among different plants. Our data also suggest that the benefits ofalpha-dioxygenase 1 for limiting aphid infestations can be enhanced by increasing the foliar abundance of linoleic acid, one of the substrates on which this enzyme can act. These findings shed light on mechanisms of basal immunity in plants, and reveal potential approaches for enhancing aphid resistance in multiple crops.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2013
Citation:
Avila, C.A., Arevalo-Soliz, M.A., Lorence, A., and Goggin, F.L. 2013. Expression of ?-DIOXYGENASE 1 in tomato and Arabidopsis contributes to plant defenses against aphids. Molecular Plant Microbe Interactions, 26(8): 977-86.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2013
Citation:
Goggin, F.L. 2013. Deciphering the molecular determinants of host plant resistance or susceptibility to aphids: impact of fatty acid desaturases. Plant Science Seminar Series, University of Missouri, Columbia, MO.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2013
Citation:
F.L. Goggin, A. Lorence, M. Meuller 2013. "Omics" approaches to understand plant resistance or susceptibility to aphids: impact of fatty acid metabolism. Invited talk, Annual Meeting of the Entomological Society of America, Austin, TX.
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2013
Citation:
Avila, C.,and Goggin, F.L. 2013. RNA-Seq Analysis of how Fatty Acid Desaturase 7 and NPR1 influence gene expression in tomato plants under aphid challenge. Annual Meeting of the Southern Section of the American Society of Plant Biologists (SS-ASPB), Little Rock, AR (Poster).
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