Source: UNIVERSITY OF ARKANSAS submitted to
FOODBORNE BACTERIAL PATHOGEN SURVIVAL AND GENOMIC RESPONSES TO FOOD PRODUCTION ENVIRONMENTS
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
TERMINATED
Funding Source
HATCH
Reporting Frequency
Annual
Accession No.
0231037
Grant No.
(N/A)
Project No.
ARK02359
Proposal No.
(N/A)
Multistate No.
(N/A)
Program Code
(N/A)
Project Start Date
Oct 1, 2012
Project End Date
Sep 30, 2017
Grant Year
(N/A)
Project Director
RICKE, ST, C..
Recipient Organization
UNIVERSITY OF ARKANSAS
(N/A)
FAYETTEVILLE,AR 72703
Performing Department
Food Science
Non Technical Summary
Salmonellosis is one of the most common foodborne diseases with a range anywhere from 1 to 4 million human infections reported each year in the United States. Further, it is one of the more costly foodborne diseases in the U.S. Given that foodborne Salmonella spp. can originate from a wide variety of food production environments, reduction of this organism at all stages of food production is critical. This project's research program reflects an integrated approach for controlling Salmonella spp. and other foodborne pathogens by examining virulence expression of Salmonella under typical food production and processing conditions.
Animal Health Component
(N/A)
Research Effort Categories
Basic
(N/A)
Applied
(N/A)
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
7125010104040%
7125010108020%
7125010110040%
Knowledge Area
712 - Protect Food from Contamination by Pathogenic Microorganisms, Parasites, and Naturally Occurring Toxins;

Subject Of Investigation
5010 - Food;

Field Of Science
1040 - Molecular biology; 1100 - Bacteriology; 1080 - Genetics;
Goals / Objectives
1.Determine the environmental factors that must be modified to prevent the early establishment of Salmonella in food production environments and virulence expression under these conditions; 2.Study the metabolism of Salmonella spp. under anaerobic conditions to better understand what factors are required for successful Salmonella spp. establishment in food production animals; 3.Screen potential prebiotic compounds that would promote beneficial bacteria in the gastrointestinal tract for humans and animals.
Project Methods
The idea here is to compare anaerobic and aerobic growth and pathogenic responses of foodborne Salmonella spp. The hypothesis is that Salmonella may physiologically behave differently when grown under one environmental condition versus the other. Our food production Salmonella spp. research projects will emphasize studies on the growth, survival and pathogenesis of Salmonella spp. under conditions encountered during food animal production and processing. We will also examine virulence activity of Salmonella spp. under these conditions.

Progress 10/01/12 to 09/30/17

Outputs
Target Audience:Salmonellosis is one of the most common foodborne diseases with a range anywhere from 1 to 4 million human infections reported each year in the United States. Further, it is one of the more costly foodborne diseases in the U.S. Given that foodborne Salmonella spp. can originate from a wide variety of food production environments, reduction of this organism at all stages of food production is critical. This project's research program reflects an integrated approach for controlling Salmonella spp. and other foodborne pathogens by examining virulence expression of Salmonella under typical food production and processing conditions. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?Postdocs and graduate students continued training on molecular techniques including microbiome analyses. How have the results been disseminated to communities of interest?Sixteen talks were given by the PI to a wide range of audiences, locally and in national forums. What do you plan to do during the next reporting period to accomplish the goals?This is the final report for this project.

Impacts
What was accomplished under these goals? Salmonella Research: A novel method was developed for the specific quantification of S. Typhimurium using an MPN combined with qPCR and a shorted incubation time (MPN-qPCR-SIT). For S. Typhimurium enumeration, dilutions of samples were transferred into three wells on a microtiter plate (same as MPN assay) and the plate was incubated for 4 h. The S. Typhimurium presence in wells was identified using a qPCR and populations were determined based on an MPN calculation. Bacterial populations were also determined with conventional MPN, plating, and qPCR to compare across independent methods. The R2 between the MPN-qPCR-SIT and MPN exhibited a high level of correlation (0.9752 in Salmonella pure culture, 0.9627 in mixed culture, and 0.9335 in mixed culture on chicken breast), suggesting that the MPN-qPCR-SIT offers a reliable alternative method for S. Typhimurium quantification. Although plating and qPCR were limited in their ability to detect low levels of S. Typhimurium (e.g. 0.18 log MPN/ml), these levels could be successfully detected with the MPN-qPCR-SIT. Chicken breast samples inoculated with S. Typhimurium were incubated at 0, 4, and 24 h and incubated samples were subjected to microbiome analysis. Both Salmonella and Enterobacteriaceae abundance was significantly increased along with incubation time; indicating that incubation could sufficiently support growth of target bacteria in the presence of a mixed community. The obvious strengths of the MPN-qPCR-SIT are 1) further confirmation step is not required, 2) the detection limit is as low as conventional MPN, but 3) is more rapid, requiring approximately 7 h to simultaneously complete quantification. Prebiotics and microbiome: When prebiotics and other fermentation substrates are delivered to animals as feed supplements, the typical goal is to improve weight gain and feed conversion. In this work, we examined pasture flock chicken cecal contents using next generation sequencing (NGS) to identify and understand the composition of the microbiome when prebiotics and fermentation substrates were supplemented. We generated 16S rRNA sequencing data for 120 separate cecal samples from groups of chickens receiving 1 of 3 prebiotics or fiber feed additives. The data indicated that respective feed additives enrich for specific bacterial community members and modulate the diversity of the microbiome. We applied synthetic learning in microbial ecology (SLiME) analysis to interpret 16S rRNA microbial community data and identify specific bacterial operational taxonomic units (OTUs) that are predictive of the particular feed additives used in these experiments. The results suggest that feed can influence microbiome composition in a predictable way, and thus diet may have indirect effects on weight gain and feed conversion through the microbiome.

Publications

  • Type: Journal Articles Status: Published Year Published: 2017 Citation: OBryan, C.A., P. Crandall, D. Jaroni, S.C. Ricke and K.E. Gibson. 2017. Assessment of nitrogen and phosphorus loads present in environments impacted by alternative poultry processing operations. Renewable Agriculture and Food Systems. 32: 33-42
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Jarvis, N.A., J.R. Donaldson, C.A. OBryan, S.C. Ricke, and P.G. Crandall. 2017. Listeria monocytogenes infection of HD11, chicken macrophage-like cells. Poultry Sci. 96: 950-956.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Roto, S.M., S.H. Park, S.I. Lee, P. Kaldhone, H.O. Pavlidis, S.B. Frankenbach, D.R. McIntyre, K. Striplin, L. Brammer, and S. C. Ricke. 2017. Effects of feeding Original XPCTM to broilers with a live coccidiosis-vaccine under industry conditions: Part 1. Growth performance and Salmonella inhibition. Poultry Sci. 1831-1837.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Park, S,H., A. Perrotta, I. Hanning, S. Diaz-Sanchez, S. Pendleton, E. Alm, and S.C. Ricke. 2017. The chicken gut microbiome changes in response to prebiotics and plum fibers. Poultry Sci. 96: 1820-1830.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: ) Dawoud, T.M., A. Khatiwari, S.H. Park, M.L. Davis, C.A. Baker, S.C. Ricke, and Y.M. Kwon. 2017. Heat survival and phenotype microarray profiling of Salmonella Typhimurium mutants. Curr. Microbiol. 74:257267.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Park, S.H., S. Roto, H. Pavlidis, D. McIntyre, K. Striplin, L. Brammer, and S.C. Ricke. 2017. Effects of feeding Original XPCTM to broilers with a live coccidiosis-vaccine under industrial conditions: Part 2. Cecal microbiota analysis. Poultry Sci. 96: 24002411.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Kim, S.A., Si H. Park, S. In. Lee, C. Owens-Hanning, and S.C. Ricke. 2017. Assessment of chicken carcass microbiome responses during processing in the presence of commercial antimicrobials using a next generation sequencing approach. Scientific Reports 7:43354 | doi: 10.1038/srep43354.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Kim, S.A., S.H. Park, S.I. Lee, and S.C. Ricke. 2017. Development of a rapid method to quantify Salmonella Typhimurium using a combination of MPN with qPCR and a shortened time incubation. Food Microbiology 65: 7-18.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Yang, Y., S.C. Ricke, G. Tellez, and Y.M. Kwon. 2017. Quantitative tracking of Salmonella Enteritidis transmission routes using barcode-tagged isogenic strains in chickens  Proof of concept study. Frontiers in Vet. Sci. 4:15. doi: 10.3389/fvets.2017.0001.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Rubinelli, P.M., S.A. Kim, S.H. Park, S.M. Roto, and S.C. Ricke. 2017. Sodium bisulfate and a sodium bisulfate/tannin mixture decreases pH when added to an in vitro incubated poultry cecal or fecal contents while reducing Salmonella Typhimurium marker strain survival and altering the microbiome. J. Environ. Sci. and Health, Part B 52: 607-615.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Park, S.H., S.A. Kim, P.M. Rubinelli, S.M. Roto and S.C. Ricke. 2017. Microbial compositional changes in broiler chicken cecal contents from birds challenged with different Salmonella vaccine candidate strains. Vaccine 35: 3204-3208.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Park, S.H., S.A. Kim, S.I. Lee, P.M. Rubinelli, S.M. Roto, H.O. Pavlidis, D.R. McIntyre, and S.C. Ricke. 2017. Original XPCTM effect on Salmonella Typhimurium and cecal microbiota from three different ages of birds when incubated in an anaerobic in vitro culture system. Frontiers in Microbiol. 8: (Article 1070). doi 10.3389/fmoicb.2017 01070.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Jarvis, N.A., C.A. O'Bryan, E.M. Martin, S.C. Ricke, M.G. Johnson, and P.G. Crandall. 2017. Further evidence of how unbuffered starvation at 4 ?C influences Listeria monocytogenes EGD-e, HCC23, F2365, and Scott A. J. Food Prot. 80: 1749-1759.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Rubinelli, R., S.A. Kim, S.H. Park, C.A. Baker, and S.C. Ricke. 2017. Characterization of single and double Salmonella methionine auxotroph strains for potential vaccine use in poultry. Frontiers in Veterinary Science 4 (Article 103): doi: 10.3389/fvets2017.00103.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Park, S.H., S.I. Lee, K. Christensen, and S.C. Ricke. 2017. Effect of commercial prebiotic on gastrointestinal microbial population and growth performance of broilers. PLoS ONE 12(8): e0182805. https://doi.org/10.1371/ journal.pone.0182805.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Kim, S.A., S.H. Park, S.I.. Lee, and S.C. Ricke. 2017. Rapid and simple method by combining FTATM card DNA extraction with two set multiplex PCR for simultaneous detection of non-157 Shiga toxin-producing Escherichia coli strains and virulence genes in food samples. Letters in Applied Microbiology 65: 482-488.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Kaldhone, P., B. Khajanchi, J. Han, R. Nayak, S.C. Ricke, and S.L. Foley. 2017. Draft genome sequences of Salmonella enterica isolates containing incompatibility group I1 plasmids from swine, poultry and human sources. Genome Announcement 5 no. 39 e01056-17, doi: 10.1128/genomeA.01056-17.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Rubinelli, P.M., S.A. Kim, S.H. Park, S.M. Roto, N.J. Nealon, E.P. Ryan, and S.C. Ricke. 2017. Differential effects of rice bran cultivars to limit Salmonella Typhimurium in chicken cecal in vitro incubations and impact on the cecal microbiome and metabolome. PLoS ONE 12(9): e0185002. https://doi.org/10.137/journal.pone0185002.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Rajan, K, Z. Shi, and S.C. Ricke. 2017. Potential application of risk strategies for understanding Salmonella contamination in ground poultry products. Crit. Revs. Microbiol. 43: 370-392.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Wu, S., S.C. Ricke, K.R. Schneider, and S. Ahn. 2017. Food safety hazards associated with ready-to-eat cookie dough and its ingredients. Food Control 73:986993.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Dawoud, T.M., M.L. Davis, S.H. Park, S.A. Kim, Y.M. Kwon, N. Jarvis, C. OBryan, Z. Shi, P.G. Crandall, and S.C. Ricke. 2017. Salmonella thermal resistance - Molecular responses. Front. Vet. Sci 4: (Article 93) doi: 10.33889/fvets.2017.00093.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Rothrock Jr., M.J., M.L. Davis, A. Locatelli, A. Bodie, T.G. McIntosh, and S.C. Ricke. 2017. Listeria occurrence in poultry flocks: Detection and potential implications. Frontiers in Veterinary Science 4 (Article 125) doi: 10.3389/fvets.2017.00125.
  • Type: Journal Articles Status: Published Year Published: 2017 Citation: Varela-Stokes, A.S., S.H. Park, S.A. Kim, and S.C. Ricke. 2017. Microbial communities in North American Ixodid ticks of veterinary and medical importance. Frontiers in Veterinary Science 4: Article 179. doi 10.3389/fvets.2017.00179.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Baxter, M., J.D. Latorre, N. Anthony, S. Dridi, D.A. Koltes, S.C. Ricke, S. Park, E.S. Greene, R. Merino, S. Bickler, B.M. Hargis, and G. Tellez. 2016 Nutritional rehabilitation responses in two genetic lines of chickens. Symposium on Gut Health in Production of Food Animals, St. Louis, MO., Nov. 14-16.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Lankford, S., S. Ricke, and D.R. Thompson. 2017. Simulating foodborne pathogens in poultry production and processing to defend against intentional contamination, Arkansas Academy of Science (AAoS) 10th Annual Meeting, University of Central Arkansas, Conway, AR., Apr 7.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Yang, Y., J.D. Latorre, Y. Kwon, P.M. Ray, X.H. Velasco, B.M. Hargis, G.I. Tellez, and S.C. Ricke. 2017. Salmonella excludes Salmonella in poultry: Confirming an old paradigm using conventional and barcode-tagged isogenic strains. 9th Annual Arkansas Association Protection Educational Conference, Holiday Inn, Springdale, AR., Sept. 19-21.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Wages, J.A., S.A. Kim, S.H. Park, S.C. Ricke, E. Oxford, R.O. Apple, and B.J. Bench. 2017. The application of next generation sequencing to evaluate the microbial ecology in commercial poultry processing environments. 9th Annual Arkansas Association Protection Educational Conference, Holiday Inn, Springdale, AR., Sept. 19-21.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Atchley, J.A., S.A. Kim, C. Knueven, L.R. Meyer, and S.C. Ricke. 2017. Assessment of sodium bisulfate as a potential antimicrobial against Salmonella Typhimurium on chicken breast meat. 9th Annual Arkansas Association Protection Educational Conference, Holiday Inn, Springdale, AR., Sept. 19-21.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Micciche, A.C., H. O. Pavlidis, and S.C. Ricke. 2017. A preliminary overview of commercialized probiotics for food animal application, research directions and future commercial trends. 9th Annual Arkansas Association Protection Educational Conference, Holiday Inn, Springdale, AR., Sept. 19-21.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Bodie, A., S.A. Kim, J. Atchely, C. Knueven, and S.C. Ricke. 2017. Potential antimicrobials for controlling Listeria monocytogenes in hotdogs. 9th Annual Arkansas Association Protection Educational Conference, Holiday Inn, Springdale, AR., Sept. 19-21.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Micciche, A.C., P. M. Rubinelli, Z. Shi, and S. C. Ricke. 2017. Comparison of DNA Extraction Methods for qPCR Detection of Pathogenic Campylobacter strains. 9th Annual Arkansas Association Protection Educational Conference, Holiday Inn, Springdale, AR., Sept. 19-21.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Kim, S.A., P. Rubinelli, S.H. Park, T. Gaydos, J. Corley, R. Raspoet, and S.C. Ricke. 2017. Evaluation of Actisaf and Safmannan effects on gastrointestinal microbiota in cecal and ileum contents of chicken broiler. Symposium on Gut Health in Production of Food Animals, Nov. 13-15, St. Louis, MO.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Kaldhone P.R., B.K. Khajanchi, J. Han J., S.C. Ricke, and S.L. Foley. 2017. Virulence evaluation of Incompatibility group 1 (IncI1) plasmids containing Salmonella enterica. South Central Branch Meeting of ASM, Little Rock, AR.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Baxter, M., B. Hargis, J.D. Latorre, S. Ricke, S.H. Park, S. Dridi, L. Greene, S. Bickler, R. Merino, and M. Tellez. 2017. Evaluating the effect of corn and/or rye based diets on gut health, bone strength and body weight in modern broiler chickens. International Poultry Scientific Forum, Atlanta, GA, Jan. 30-31st.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Ricke, S. S.I. Lee, S.H. Park, K. Hiett, H. Sellers, and M. Rothrock. 2017. Microbial population analysis of broilers in different flock and embryonic ages from GI, yolk and egg wash via Illumina Miseq and QIIME pipeline. International Poultry Scientific Forum, Atlanta, GA, Jan. 30-31st.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Ricke, S., S.I. Lee, S.H. Park, S. Roto, P. Rubinelli, H. Pavlidis, and D. McIntyre. 2017. Impact of incubation time and addition of original-XPC on the microbiota in an in vitro broiler cecal bioassay. International Poultry Scientific Forum, Atlanta, GA, Jan. 30-31st.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Rubinelli, P., S.H. Park, H. Pavlidis, D. McIntyre, and S. Ricke. 2017. Inhibition of Campylobacter jejuni by original XPCTM in an anaerobic mixed fecal in vitro culture system. International Poultry Scientific Forum, Atlanta, GA, Jan. 30-31st.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Park, S.H., S.A. Kim, P. Rubinelli, C.A. Baker, and S.C. Ricke. 2017. Effect of Salmonella vaccine strains on broiler chicken cecal microbiota. International Assoc. Food Prot. Annual Meeting, Tampa, FL July. 9-12.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Kim, S.A., S.H. Park, S.I. Lee, and S.C. Ricke. 2017. A novel Salmonella Typhimurium quantification approach in a mixed cell culture spiked in chicken breast. International Assoc. Food Prot. Annual Meeting, Tampa, FL July. 9-12.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Shi, Z., P.M. Rubinelli, and S.C. Ricke. 2017. Poultry serovar Salmonella biofilm formation at the air-liquid interface in media of differing compositions and environmental incubation conditions. Poultry Science Association Annual Meeting, Orlando, FL, July 16th -20th.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Kim, S.A. S.H. Park, A.D. Wolfenden, B.M. Hargis, H.O. Pavlidis, D.R. McIntrye, and S.C. Ricke. 2017. Characterization of mixed bacterial populations in chicken ceca recovered from colonies on Campylobacter selective commercial media using 16S rRNA next generation sequencing. Poultry Science Association Annual Meeting, Orlando, FL, July 16th -20th.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Baxter, M., J.D. Latorre, S.H., S. Ricke, X. Sun, B. Hargis and G. Tellez. 2017. Relationship between the microbiome in different sections of the gastrointestinal tract of broiler chickens fed a corn versus a rye based diets. Poultry Science Association Annual Meeting, Orlando, FL, July 16th -20th.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Kim, S.A., S.H. Park, P.M. Rubinelli, and S.C. Ricke. 2017. Ability of rice brans to control Salmonella Typhimurium in chicken ceca and effects on cecal microbiota. The Korean Society of Food Hygiene and Safety Seoul, South Korea, Nov. 9th-10th.
  • Type: Books Status: Published Year Published: 2017 Citation: Ricke, S.C. (Ed.) 2017. Achieving Sustainable Production of Poultry Meat- Volume 1 Safety, Quality and Sustainability. Burleigh Dodd Publishing, Cambridge, UK. 480 pp.
  • Type: Books Status: Published Year Published: 2017 Citation: Ricke, S.C., G.G. Atungulu, S.H. Park, and C.E. Rainwater (Eds.) 2017. Food and Feed Safety Systems and Analysis. Elsevier Inc., San Diego, CA 406 pp.
  • Type: Book Chapters Status: Published Year Published: 2017 Citation: Ricke, S.C., A.V.S. Perumalla, and N.S. Hettiarachchy. 2017. Chapter 5. Alternatives to antibiotics in preventing zoonoses and other pathogens in poultry: Prebiotics and related compounds. In: S.C. Ricke (Ed.) Achieving Sustainable Production of Poultry Meat- Volume 1 Safety, Quality and Sustainability. Burleigh Dodd Publishing, Cambridge, UK. pp. 87-108.
  • Type: Book Chapters Status: Published Year Published: 2017 Citation: Luj�n-Rhenals, D., R. Morawicki, E.J. Van Loo, and S.C. Ricke. 2017. Chapter 19. Energy and water use in poultry processing. In: S.C. Ricke (Ed.) Achieving Sustainable Production of Poultry Meat- Volume 1 Safety, Quality and Sustainability. Burleigh Dodd Publishing, Cambridge, UK, pp. 389-409.
  • Type: Book Chapters Status: Published Year Published: 2017 Citation: Luj�n-Rhenals, D., R. Morawicki, E.J. Van Loo, and S.C. Ricke. 2017. Chapter 20. Waste management and emissions in poultry processing. In: S.C. Ricke (Ed.) Achieving Sustainable Production of Poultry Meat- Volume 1 Safety, Quality and Sustainability. Burleigh Dodd Publishing, Cambridge, UK, pp. 411-431.
  • Type: Book Chapters Status: Published Year Published: 2017 Citation: Ricke, S.C. 2017. Chapter 22. Microbial ecology of eggs: A focus on Salmonella and microbial contamination in post-harvest table shell egg production. In: Anderson de Souza SantAna (Ed.) Quantitative Microbiology in Food Processing  Modeling the Microbial Ecology. John Wiley & Sons, Ltd., Oxford, UK. pp. 416-441.
  • Type: Book Chapters Status: Published Year Published: 2017 Citation: OBryan, C.A., P.G. Crandall, and S. C. Ricke. 2017. Chapter 6. Antimicrobial resistance in foodborne pathogens. In: S.C. Ricke, G.G. Atungulu, S.H. Park, and C.E. Rainwater (Eds.) Food and Feed Safety Systems and Analysis. Elsevier Inc., San Diego, CA, pp 99-115.
  • Type: Book Chapters Status: Published Year Published: 2017 Citation: Swaggerty, C.L., N. Corcionivoschi, S.C. Ricke, and T.R. Callaway. 2017. Chapter 7. The first 30 years of shiga toxin-producing Escherichia coli (STEC) in cattle production: Incidence, pre-harvest ecology, and management. In: S.C. Ricke, G.G. Atungulu, S.H. Park, and C.E. Rainwater (Eds.) Food and Feed Safety Systems and Analysis. Elsevier Inc., San Diego, CA pp. 117-131.
  • Type: Book Chapters Status: Published Year Published: 2017 Citation: Swaggerty, C.L., N. Corcionivoschi, S.C. Ricke, and T.R. Callaway. 2017. Chapter 8. The first 30 years of shiga toxin-producing Escherichia coli (STEC) in cattle production: Pre-harvest intervention strategies. In: S.C. Ricke, G.G. Atungulu, S.H. Park, and C.E. Rainwater (Eds.) Food and Feed Safety Systems and Analysis. Elsevier Inc., San Diego, CA pp. 133-151.
  • Type: Book Chapters Status: Published Year Published: 2017 Citation: Ricke, S.C., T.M. Dawoud, Z. Shi, P. Kaldhone, and Y.M. Kwon. 2017. Chapter 9. Foodborne Salmonella in Laying Hens and Egg Production. In: S.C. Ricke, G.G. Atungulu, S.H. Park, and C.E. Rainwater (Eds.) Food and Feed Safety Systems and Analysis. Elsevier Inc., San Diego, CA pp. 153-171.
  • Type: Book Chapters Status: Published Year Published: 2017 Citation: Zhuang, X., C. Zhao, K. Liu, P. Rubinelli, S.C. Ricke, and G.G. Atungulu. 2017. Chapter 10. Cereal grain fractions as potential sources of prebiotics: Current status, opportunities, and potential applications. In: S.C. Ricke, G.G. Atungulu, S.H. Park, and C.E. Rainwater (Eds.) Food and Feed Safety Systems and Analysis. Elsevier Inc., San Diego, CA pp. 173-191.
  • Type: Book Chapters Status: Published Year Published: 2017 Citation: OBryan, C.A., S.J. Pendleton, S.C. Ricke, and P.G. Crandall. 2017. Chapter 11. Interventions to reduce Shiga-toxin producing Escherichia coli on beef carcasses at slaughter. In: S.C. Ricke, G.G. Atungulu, S.H. Park, and C.E. Rainwater (Eds.) Food and Feed Safety Systems and Analysis. Elsevier Inc., San Diego, CA pp.195-212.
  • Type: Book Chapters Status: Published Year Published: 2017 Citation: Saldivar, J., M.L. Davis, M.J. Johnson, and S.C. Ricke. 2017. Chap. 13. Listeria monocytogenes and growth at low temperatures: Mechanisms and implications for foodborne disease. In: S.C. Ricke, G.G. Atungulu, S.H. Park, and C.E. Rainwater (Eds.) Food and Feed Safety Systems and Analysis. Elsevier Inc., San Diego, CA pp. 227-248.
  • Type: Book Chapters Status: Published Year Published: 2017 Citation: OBryan, C.A., O. K. Koo, M. L. Sostrin, S.C. Ricke, P.G. Crandall, and M.G. Johnson. 2017. Chapter 15. Characteristics of bacteriocins and use as food antimicrobials in the United States. In: S.C. Ricke, G.G. Atungulu, S.H. Park, and C.E. Rainwater (Eds.) Food and Feed Safety Systems and Analysis. Elsevier Inc., San Diego, CA, pp. 273-286.
  • Type: Book Chapters Status: Published Year Published: 2017 Citation: Park, S.H., M. Aydin, P. Fan, S. Lee, L. Teng, S.A. Kim, S. Ahn, S.C. Ricke, Z. Shi and K.C. Jeong. Chapter 16. Detection strategies for foodborne Salmonella and prospects for utilization of whole genome sequencing approaches. In: S.C. Ricke, G.G. Atungulu, S.H. Park, and C.E. Rainwater (Eds.) Food and Feed Safety Systems and Analysis. Elsevier Inc., San Diego, CA pp. 289-308.
  • Type: Book Chapters Status: Published Year Published: 2017 Citation: Blevins, R., S.A. Kim, S.H. Park, R. Rivera, and S.C. Ricke. Chap. 18. Historical, current and future prospects for food safety in poultry product processing systems. In: S.C. Ricke, G.G. Atungulu, S.H. Park, and C.E. Rainwater (Eds.) Food and Feed Safety Systems and Analysis. Elsevier Inc., San Diego, CA pp. 323-345.
  • Type: Book Chapters Status: Published Year Published: 2017 Citation: Ricke, S.C., J. Hacker, K. Yearkey, Z. Shi, S.H. Park, and C. Rainwater. 2017. Chap. 19. Unravelling food production microbiomes: Concepts and future directions. In: S.C. Ricke, G.G. Atungulu, S.H. Park, and C.E. Rainwater (Eds.) Food and Feed Safety Systems and Analysis. Elsevier Inc., San Diego, CA pp. 347-374.
  • Type: Book Chapters Status: Published Year Published: 2017 Citation: Thompson, D.R., C. Rainwater, J. Di, and S.C. Ricke. Student cross-training opportunities for combining food, transportation, and critical infrastructure cybersecurity into an academic food systems education program. In: S.C. Ricke, G.G. Atungulu, S.H. Park, and C.E. Rainwater (Eds.) Food and Feed Safety Systems and Analysis. Elsevier Inc., San Diego, CA pp. 375-391.


Progress 10/01/15 to 09/30/16

Outputs
Target Audience:Salmonellosis is one of the most common foodborne diseases with a range anywhere from 1 to 4 million human infections reported each year in the United States. Further, it is one of the more costly foodborne diseases in the U.S. Given that foodborne Salmonella spp. can originate from a wide variety of food production environments, reduction of this organism at all stages of food production is critical. This project's research program reflects an integrated approach for controlling Salmonella spp. and other foodborne pathogens by examining virulence expression of Salmonella under typical food production and processing conditions. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?Postdocs and graduate students continued training on molecular techniques including microbiome analyses. How have the results been disseminated to communities of interest?Sixteentalks were given by the PIto a wide range of audiences, locally and in national forums. What do you plan to do during the next reporting period to accomplish the goals?Conitnue research progress.

Impacts
What was accomplished under these goals? Salmonella research: The overall goal of this study was to compare conventional plating with molecular methods for quantitating attached representative strains for Salmonella Typhimurium and Heidelberg on reusable plastic containers (RPC) coupons, respectively. We attached Salmonella enterica serovar Typhimurium ATCC 14028 and serovar Heidelberg SL486 (parent and an antibiotic resistant marker strain) to plastic coupons (2.54 cm squared) derived from previously used shipping containers by growing for 72 h in tryptic soy broth. The impact of the concentration of sanitizer on log reductions between unsanitized and sanitized coupons was evaluated by exposing attached S. Typhimurium cells to sodium hypochlorite (NaClO). Differences in sanitizer effectiveness between serovars were also evaluated with attached S. Typhimurium compared to attached S. Heidelberg populations after being exposed to peracetic acid (PAA). Treatment with NaClO caused an average of 2.73 log CFU of S. Typhimurium per coupon removed with treatment at 200 ppm while 3.36 log CFU were removed at higher (200,000) ppm. Treatment with PAA caused an average of 2.62 log CFU removed for S. Typhimurium and 1.41 log CFU for S. Heidelberg (parent) and 1.61 log CFU (marker). Lastly, scanning electron microscopy (SEM) was used to visualize cell attachment and coupon surface topography. SEM images showed that remaining attached cell populations were visible even after sanitizer application. Conventional plating and qPCR yielded similar levels of enumerated bacterial populations indicating a high concordance between the two methods. Therefore, qPCR could be used for the rapid quantification of Salmonella attached on RPC. Prebiotics and gut microbiome: The purpose of this study was to evaluate the difference in microbial populations from naked neck chicken ceca fed with commercial prebiotics derived from brewer's yeast cell wall via an Illumina MiSeq platform. A total of 147 day-of-hatch naked neck chickens were distributed into 3 groups consisted of 1) C: control (no prebiotic), 2) T1: Biolex1 MB40 with 0.2%, and 3) T2: Leiber1 ExCel with 0.2%, consistently supplemented prebiotics during the experimental period. At 8 weeks, a total of 15 birds from each group were randomly selected and ceca removed for DNA extraction. The Illumina Miseq platform based on V4 region of 16S rRNA gene was applied for microbiome analysis. Both treatments exhibited limited impact on the microbial populations at the phylum level, with no significant differences in the OTU number of Bacteroidetes among groups and an increase of Proteobacteria OTUs for the T1 (Biolex1 MB40) group. In addition there was a significant increase of genus Faecalibacterium OTU, phylum Firmicutes. According to the development of next generation sequencing (NGS), microbiome analysis based on 16S rRNA gene proved to be informative on the prebiotic impact on poultry gut microbiota in pasture-raised naked neck birds.

Publications

  • Type: Books Status: Published Year Published: 2016 Citation: Ricke, S.C. and R.K. Gast (Eds.). 2016. Producing Safe Eggs-The Microbial Ecology of Salmonella. Elsevier, Inc., San Diego, CA. 436 pp.
  • Type: Book Chapters Status: Published Year Published: 2016 Citation: Dawoud, T.M., Z. Shi, Y.M. Kwon, and S.C. Ricke. 2016. Chapter 7. Overview of salmonellosis and foodborne Salmonella: Historical and current perspectives. In S.C. Ricke and R.K. Gast (Eds.). Producing Safe Eggs-The Microbial Ecology of Salmonella. Elsevier, Inc., San Diego, CA. pp. 113-138.
  • Type: Book Chapters Status: Published Year Published: 2016 Citation: Kaldhone, P.R., S. L. Foley, and S.C. Ricke. 2016. Chapter 12. Salmonella Heidelberg in layer hens and egg production: Incidence and potential issues. In S.C. Ricke and R.K. Gast (Eds.). Producing Safe Eggs-The Microbial Ecology of Salmonella. Elsevier, Inc., San Diego, CA. pp. 235-256.
  • Type: Book Chapters Status: Published Year Published: 2016 Citation: Callaway, T.R., T.S. Edrington, J.A. Byrd, D.J. Nisbet, and S.C. Ricke. 2016. Chapter 15. Use of direct fed microbials in layer hen production-Performance response and Salmonella control. In S.C. Ricke and R.K. Gast (Eds.). Producing Safe Eggs-The Microbial Ecology of Salmonella. Elsevier, Inc., San Diego, CA. pp. 301-322.
  • Type: Book Chapters Status: Published Year Published: 2016 Citation: Ricke, S.C. 2016. Chapter 16. Gastrointestinal ecology of Salmonella Enteritidis in laying hens and intervention by prebiotic and non-digestible carbohydrate dietary supplementation. In S.C. Ricke and R.K. Gast (Eds.). Producing Safe Eggs-The Microbial Ecology of Salmonella. Elsevier, Inc., San Diego, CA. pp. 323-345.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2016 Citation: Yearkey, K., J. Hacker, C. Rainwater, and S.C. Ricke. 2016. An illustration of machine learning for the identification of bacteria through DNA sequencing data. 8th Annual Arkansas Association for Food Protection Educational Conference, Chancellor Hotel, Fayetteville, AR, Sept. 13-15.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2016 Citation: Kaldhone, P.R., J. Han, R. Nayak, S.L. Foley, and S.C. Ricke. 2016. Evaluation of the genetics and functionality of Incompatibility1 (IncI1) plasmids from Salmonella enterica. 8th Annual Arkansas Association for Food Protection Educational Conference, Chancellor Hotel, Fayetteville, AR, Sept. 13-15.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2016 Citation: Kaldhone, P.R., J. Deck, B.K. Khajanchi , R. Nayak, S.L. Foley, and S.C. Ricke. 2016. Virulence assessment of Salmonella enterica containing incompatibility group 1 (IncI1) plasmids. 8th Annual Arkansas Association for Food Protection Educational Conference, Chancellor Hotel, Fayetteville, AR, Sept. 13-15.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2016 Citation: Baxter, M., S.C. Ricke, J.D. Latorre, B. M. Hargis, S. Bickler, S. H. Park, S. Dridi, R. Merino, and G. Tellez. 2016. Evaluating the effect of corn and/or rye based diets on gut health, bone strength and body weight in broiler chickens. 8th Annual Arkansas Association for Food Protection Educational Conference, Chancellor Hotel, Fayetteville, AR, Sept. 13-15.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2016 Citation: Baxter, M., J.D. Latorre, N. Anthony, S. Dridi, D.A. Koltes, S.C. Ricke, S. Park, E.S. Greene, R. Merino, S. Bickler, B.M. Hargis, and G. Tellez. 2016 Nutritional rehabilitation responses in two genetic lines of chickens. Symposium on Gut Health in Production of Food Animals, St Louis, MO., Nov. 14-16.
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Baker, C.A., P.M. Rubinelli, S. H. Park, F.Carbonero, and S.C. Ricke. 2016. Shiga toxin-producing Escherichia coli in food: Incidence, ecology, and detection strategies in the United States. Food Control 59: 407-419.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2016 Citation: Park, S.H., S.I. Lee, and S.C. Ricke. 2016. In-depth analysis of broiler chicken gut microbiome responses over time fed a commercial yeast-based prebiotic. Amer. Soc. Microbiol. General Annual Meeting, Boston, MA, June 16-20.
  • Type: Book Chapters Status: Published Year Published: 2016 Citation: OBryan, C.A., S.C. Ricke, and P.G. Crandall. 2016. Chapter 18. Chemical and physical sanitation and pasteurization methods for intact eggs. In S.C. Ricke and R.K. Gast (Eds.). Producing Safe Eggs-The Microbial Ecology of Salmonella. Elsevier, Inc., San Diego, CA. pp. 373-390.
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Lee, S.I., S.H. Park, and S.C. Ricke. 2016. Assessment of cecal microbiota, integrons, fermentation, and Salmonella frequency in conventionally raised broilers in response to a commercial prebiotic. Poultry Sci. 95:144153.
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Sanad, Y.M., K. Johnson, S.-H. Park, B. Shaheen, J. Han, S.L. Foley, P.B. Kenney, P.B., S. Ricke, and R. Nayak. 2016. Molecular characterization of Salmonella enterica serovars isolated from a turkey production facility in the absences of selective antimicrobial pressure. Foodborne Pathogens and Disease 13: 80-87.
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Baker, C.A., S.H. Park, S.A. Kim, P.M. Rubinelli, S.M. Roto, S.I. Lee, S.Ramsaroop, M. Miller, and S.C. Ricke. 2016. Formalin-fixed cells as an internal standard approach for the detection and quantitative assessment of shiga toxin-producing Escherichia coli (STEC). Food Control 63:76-82.
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Ghosh, A., S.C. Ricke, and K.E. Gibson. 2016. Application of essential oil compounds and bacteriophage to control Staphylococcus aureus. Curr. Microbiol. 72: 426435.
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Howton, J., E. Keifer, C.A. Murphy, S. Sirsat, C.A. OBryan, S.C. Ricke, P.G. Crandall, and J. Neal. 2016. A comparison of online food safety training programs using the customizable tool for online training evaluation. Food Control 59: 82-87.
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Herzig, G.P.D., M. Aydin, S. Dunigan, P. Shah, K. C. Jeong, S.H. Park, S.C. Ricke, and S. Ahn. 2016. Magnetic bead-based immunoassay coupled with tyramide signal amplification for detection of Salmonella in foods. J. Food Safety 36: 383391.
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Koo, O.-K., H.-J. Kim, C.A. Baker, S.H. Park, and S.C. Ricke. 2016. Comparison of the microbial diversity in Korean ground beef products with PCR denaturing gradient gel electrophoresis (DGGE) and 454 pyrosequencing analyses. Food Biotechnol. 30: 63-77.
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Koo, O.K., H. Kim, H.J. Kim, C.A. Baker, and S.C. Ricke. 2016. Bacterial community analysis of tatsoi cultivated by hydroponics. J. Environ. Sci. Health, Part B 51: 490496.
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Park, S.H., S.I. Lee, and S.C. Ricke. 2016. Microbial populations in naked neck chicken ceca raised on pasture flock fed with commercial yeast cell wall prebiotics via an Illumina MiSeq platform. PloS ONE 11(3): e0151944. doi:10.1371/journal.pone.0151944.
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Koo, O.-K., C.A. Baker, H.J. Kim, S.H. Park, and S.C. Ricke. 2016. Metagenomic assessment of the microbial diversity in ground pork products from South Korean Markets. J. Environ. Sci. Health, Part B 51: 622627.
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Shi, Z., C.A. Baker, S.I. Lee, S.H. Park, S.A. Kim, and S.C. Ricke. 2016. Comparison of methods for quantitating Salmonella enterica Typhimurium and Heidelberg strain attachment to reusable plastic shipping container coupons and preliminary assessment of sanitizer efficacy. J. Environ. Sci. Health, Part B 51: 602608.
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Sharma, S., J.P. Adams, R. Sakul, E.M. Martin, S.C. Ricke, K.E. Gibson, and D.J. Carrier. 2016. Loblolly pine (Pinus taeda L.) essential oil yields affected by environmental and physiological changes. Journal of Sustainable Forestry 35: 417-430.
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Rubinelli, P., S. Roto, S.A. Kim, Si H. Park, H.O. Pavlidis, D. McIntyre, and S.C. Ricke. 2016. Reduction of Salmonella Typhimurium by fermentation metabolites of Diamond V Original XPC in an in vitro anaerobic mixed culture. Front. Vet. Sci. 3:83. doi: 10.3389/fvets.2016.00083.
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Baker, C.A., P.M. Rubinelli, S.H. Park, and S.C. Ricke. 2016. Immuno-based detection of shiga toxin-producing pathogenic Escherichia coli  A review on current approaches and potential strategies for optimization. Crit. Revs. Microbiol. 42: 656675.
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Kwon, Y.M., S.C. Ricke, and R.K. Mandal. 2016. Transposon sequencing: Methods and expanding applications. Appl. Micro. Biotechnol. 100: 3143.
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Chalova, V.I., J.H. Kim, P.H. Patterson, S.C. Ricke, and W.K. Kim. 2016. Reduction of nitrogen excretion and emission in poultry: A review for organic poultry. J. Environ. Sci. Health, Part B 51: 230-235.
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Jarvis, N.A., C.A. OBryan, S.C. Ricke, M.G. Johnson, and P.G. Crandall. 2016. A review of minimal and defined media for growth of Listeria monocytogenes. Food Control 66: 256-269.
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Jarvis, N.A., C.A. OBryan, T. M. Dawoud, S. H. Park, Y.M. Kwon, P.G. Crandall, and S.C. Ricke. 2016. An overview of Salmonella thermal destruction during food processing and preparation. Food Control 68: 280-290.
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Chalova, V.I., J.H. Kim, P.H. Patterson, S.C. Ricke, and W.K. Kim. 2016. Reduction of nitrogen excretion and emission in poultry: A review for conventional poultry. Worlds Poultry Sci. J. 72: 509-519.
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Adam, B.D., R. Holcomb, M. D. Buser, B. Mayfield, J. Thomas, C.A. OBryan, P.G. Crandall, and S.C. Ricke. 2016. Enhancing food safety, product quality, and value-added in food supply chains using whole-chain traceability. The International Food and Agribusiness Management Review 19 (Issue A): 191-214.
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Roto SM, Y.M. Kwon and S.C. Ricke. 2016. Applications of in ovo technique for the optimal development of the gastrointestinal tract and the potential influence on the establishment of its microbiome in poultry. Front. Vet. Sci. 3:63. doi: 10.3389/fvets.2016.00063.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2016 Citation: Roto, S., P. Rubinelli, S. H. Park, and S.C. Ricke. 2016. Effect of original- XPCTM on the cecal microbiome of broilers influencing the survival of Salmonella in an anaerobic in vitro mixed culture assay-fed broilers. International Poultry Scientific Forum, Atlanta, GA, Jan. 26-27th.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2016 Citation: Wages, J., J. Handley, S. H. Park, and S.C. Ricke. 2016. Comparison of indicator microbial populations occurring during multiple stages of commercial poultry carcass processing by conventional plating method and next generation sequencing. International Poultry Scientific Forum, Atlanta, GA, Jan. 26-27th.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2016 Citation: Rubinelli, P.M., S.M. Roto, S.H. Park, and S.C. Ricke. 2016. Effect of feeding original XPC" on Salmonella populations in the cecum (hindgut) of experimentally Salmonella-challenged 6 weekold broiler chickens. International Poultry Scientific Forum, Atlanta, GA, Jan. 26-27th.
  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Roto, S.M., P.M. Rubinelli, S. H. Park, and S.C. Ricke. 2016. Effects of Original XPCTM and broiler maturity on Salmonella survival and cecal microbiome modulation utilizing an anaerobic in vitro mixed culture assay. Poultry Science Association Annual Meeting, New Orleans, LA, July 11th -14th.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2016 Citation: Kim, S.A., S.H. Park, S.I. Lee, and S.C. Ricke. 2016. Assessment of different microbial populations on chicken carcasses at various first processing steps using a next generation sequencing approach. Poultry Science Association Annual Meeting, New Orleans, LA, July 11th -14th.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2016 Citation: Kim, S.A., S.H. Park, and S.C. Ricke. 2016. Development of a rapid method to quantify Salmonella Typhimurium using a combination of MPN and qPCR with a shortened enrichment time. International Assoc. Food Prot. Annual Meeting, St. Louis, MO, July 31st- Aug. 3rd.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2016 Citation: Park, S.H., S.A. Kim, S.I. Lee, P. Rubinelli, S. Roto, Z. Shi, C. Owens-Hanning, and S.C. Ricke. 2016. Evaluation of antimicrobial effects on pathogen reduction on chicken carcass during first processing. International Assoc. Food Prot. Annual Meeting, St. Louis, MO, July 31st- Aug. 3rd.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2016 Citation: Clark, J., P. Crandall, N. Jarvis, C. O'Bryan, and S. Ricke. 2016. Comparing growth kinetics of Listeria spp. isolates from pastured poultry to varied sources. International Assoc. Food Prot. Annual Meeting, St. Louis, MO, July 31st- Aug. 3rd.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2016 Citation: Gavron, N.A., J.V. Stokes, S.H. Park, S.C. Ricke, J.K. Lee, K.J. Graham, S. Cannalioto, and A.S. Varela-Stokes. 2016. Refining approaches to assess microbiota and low-profile pathogens in the tick vector, Amblyomma maculatum. Merial-NIH National Veterinary Scholars Symposium. July 28-21. The Ohio State University College of Veterinary Medicine.


Progress 10/01/14 to 09/30/15

Outputs
Target Audience:Salmonellosis is one of the most common foodborne diseases with a range anywhere from 1 to 4 million human infections reported each year in the United States. Further, it is one of the more costly foodborne diseases in the U.S. Given that foodborne Salmonella spp. can originate from a wide variety of food production environments, reduction of this organism at all stages of food production is critical. This project's research program reflects an integrated approach for controlling Salmonella spp. and other foodborne pathogens by examining virulence expression of Salmonella under typical food production and processing conditions. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?Postdocs and graduate students continued training on molecular techniques including microbiome analyses How have the results been disseminated to communities of interest?Eight talks were given to wide range of audiences, locally and national forums. What do you plan to do during the next reporting period to accomplish the goals?Continue research progress

Impacts
What was accomplished under these goals? Salmonella research: Theaims of this research were to compare the aerobic growth responses of selected predominant foodborne Salmonella serovars, and evaluate how the spent media from different serovars affects the growth of a well-characterized S. Typhimurium strain. Growth responses were similar for most strains in spent media except for S. Typhimurium (ATCC 14028), which exhibited a decrease in growth in the presence of S. Heidelberg (ARI-14) spent media. This research will provide a better understanding of the growth differences among several Salmonella serovars in nutrient limited spent media. Determining the compounds, and possibly the signals that are responsible for the inhibitory action of SH spent media on ST (ATCC 14028) will likely involve the assessment of SH genetically, as well as the compounds present in the spent media. Additionally, determining the genes expression levels that correlate with the inhibitory action will provide guidance to the underlying mechanisms and interactions between these two Salmonella strains, and potentially provide insight into the overall interactions among these Salmonella serovars. To the best of our knowledge, this is the first experiment that studies the effect of SH spent media on the growth response of ST, and whether or not there is a competitive interaction between these two serovars. Prebiotics and gut microbiome: Rice bran has been investigated for its prebiotic properties. A prebiotic has been defined as "a non-digestible food ingredient that beneficially affects the host by selectively stimulating the growth and/or activity of one or a limited number of bacteria in the colon". Several studies have demonstrated that Salmonella colonization in the animal gut can be reduced by inclusion of rice bran in the diet, which acts as a source of oligosaccharides for the promotion of anaerobic microorganisms in the lower gastrointestinal tract. These microbes are able to combat pathogens such as Salmonella that are often commensals in chicken and other poultry and that can contaminate chicken meat during evisceration at the meat packaging plant. We have recently compared inhibition of Salmonella by the bran fraction from several different cultivars of rice, including two grown in Arkansas, in an in vitro anaerobic mixed culture system (bioassay) that mimics conditions within the hindgut (ceca) of broiler chickens. Our results indicate that bran from Arkansas-grown purebred Lakast rice and from Calrose rice exhibit superior inhibition of Salmonella in comparison to the three other rice brans tested, Jasmine, RedWalls, and an Arkansas hybrid rice. The Arkansas-grown Lakast was similar in its ability to inhibit Salmonella as compared to a commercial prebiotic. The future goals of this research will be to screen additional Arkansas-grown rice varieties for the reduction of Salmonella in our in vitro system, and to test the best-performing varieties in a feeding trial with a Salmonella challenge in live chickens. .

Publications

  • Type: Journal Articles Status: Published Year Published: 2015 Citation: Patterson, J.A. and S. C. Ricke. 2015. Effect of ethanol and methanol on growth of ruminal bacteria Selenomonas ruminantium and Butyrivibrio fibrisolvens. J. Environ. Sci. Health, Part B, 50: 62-67.
  • Type: Journal Articles Status: Published Year Published: 2015 Citation: Beers, K., J. Ferguson, S. H. Park, P. Cook, C.A. Baker , M. Miller, D. Caldwell, S. Ramsaroop, and S. C. Ricke. 2015. Comparison of the RAPID-B� flow cytometer and the BAX� system for the detection of non-O157 shiga toxin-producing Escherichia coli (STEC) in beef products. Food Control 50: 72-75.
  • Type: Journal Articles Status: Published Year Published: 2015 Citation: Milillo, S.R., B. Lungu, C. A. OBryan, S.E. Dowd, A. Muthaiyan, M. G. Johnson, P.G. Crandall, and S. C. Ricke. 2015. Listeria monocytogenes batch culture growth response to metabolic inhibitors. J. Environ. Sci. Health, Part B, 50: 146-150.
  • Type: Journal Articles Status: Published Year Published: 2015 Citation: Park, S.H. and S. C. Ricke. 2015. Development of multiplex and quantitative PCR assays for simultaneous detection of Salmonella genus, Salmonella subspecies I, S. Enteritidis, S. Heidelberg, and S. Typhimurium. J. Appl. Microbiol. 118, 152-160.
  • Type: Journal Articles Status: Published Year Published: 2015 Citation: Rivera Calo, J., C.A. Baker, S.H. Park, and S.C. Ricke. 2015. Specificity of Salmonella Typhimurium strain (ATCC 14028) growth responses to Salmonella serovar-generated spent media. J. Environ. Health Part B 50: 423429.
  • Type: Journal Articles Status: Published Year Published: 2015 Citation: Mertz, A.W., C.A. OBryan, P.G. Crandall, S.C. Ricke, and R. Morawicki. 2015. The elimination of Listeria monocytogenes attached to stainless steel or aluminum using multiple hurdles. J. Food Sci. 80: M1557-M1562.
  • Type: Journal Articles Status: Published Year Published: 2015 Citation: Kwak, H.-L., S.-K. Han, S. Park, S. H. Park, J.-Y. Shim, M. Oh, S.C. Ricke, and H.-Y. Kim. 2015. Development of a rapid and accurate identification method for Citrobacter species isolated from pork products using a Matrix-Assisted Laser-Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS). Journal of Microbiology and Biotechnology 25: 1537-1541.
  • Type: Journal Articles Status: Published Year Published: 2015 Citation: Rubinelli, P.M., S.I. Lee, S.M. Roto, S.H. Park, and S.C. Ricke. 2015. Regulated expression of virulence gene mviN provides protective immunity and colonization control of Salmonella in poultry. Vaccine 33: 53655370.
  • Type: Journal Articles Status: Published Year Published: 2015 Citation: Jarvis, N., C.A. OBryan, S.C. Ricke, and P.G. Crandall. 2015. The functionality of plum ingredients in meat products: a review. Meat Science 102: 4148.
  • Type: Journal Articles Status: Published Year Published: 2015 Citation: Roto, S.M., P. M. Rubinelli, and S.C. Ricke. 2015. An introduction to the avian gut microbiota and the effects of yeast-based prebiotic compounds as potential feed additives. Front. Vet. Sci. 2 (Article 28): 1-18. doi: 10.3389/fvets.2015.00028.
  • Type: Journal Articles Status: Published Year Published: 2015 Citation: Ricke, S.C. 2015. Potential of fructooligosaccharide prebiotics in alternative and nonconventional poultry production systems. Poultry Sci. 94:14111418.
  • Type: Book Chapters Status: Published Year Published: 2015 Citation: OBryan, C.A., P.G. Crandall, S.C. Ricke, and J.B. Ndahetuye. 2015. Chapter 6. Lactic acid bacteria as antimicrobials in food products: types and mechanisms of action. In Handbook of Natural Antimicrobials for Food Safety and Quality. Matt Taylor (Ed.), Woodhead Publishing Ltd., Cambridge, UK. pp 117-136.
  • Type: Book Chapters Status: Published Year Published: 2015 Citation: OBryan, C.A., P.G. Crandall, S.C. Ricke, and J.B. Ndahetuye. 2015. Chapter 7. Lactic acid bacteria as antimicrobials in food products: analytical methods and applications. In Handbook of Natural Antimicrobials for Food Safety and Quality. Matt Taylor (Ed.), Woodhead Publishing Ltd., Cambridge, UK. pp. 137-151.
  • Type: Book Chapters Status: Published Year Published: 2015 Citation: OBryan, C.A., E.S. Whaley, S.R. Milillo, P.G. Crandall, M.G. Johnson, and S.C. Ricke. 2015. Chapter 15. Use of bacteriocins and essential oils for the control of Listeria monocytogenes in processed foods. In: Microbial Food Safety and Preservation Techniques, Eds. V Ravishankar Rai and A Jamuna Bai, CRC Press/Taylor & Francis Group, Boca Raton, FL., pp. 287-304.
  • Type: Book Chapters Status: Published Year Published: 2015 Citation: Ricke, S.C., and S. Saengkerdsub. 2015. Chapter 19. Bacillus probiotics and biologicals for improving animal and human health: Current applications and future prospects In: V. Ravishankar Rai and A Jamuna Bai (Eds.) Beneficial Microbes in Fermented and Functional Foods, CRC Press/Taylor & Francis Group, Boca Raton, FL pp. 341-360.
  • Type: Book Chapters Status: Published Year Published: 2015 Citation: Ricke, S.C. and J.Rivera Calo. 2015. Chapter 5. Antibiotic resistance in pathogenic Salmonella. In: Chin-Yi Chen, Xianghe Yan, and Charlene Jackson (Eds.). Antimicrobial Resistance and Food Safety: Methods and Techniques. Academic Press/Elsevier Science, Amsterdam, The Netherlands. pp. 37-53.
  • Type: Book Chapters Status: Published Year Published: 2015 Citation: Jarvis, N.A., P.G. Crandall, C.A. OBryan, and S.C. Ricke. 2015. Antimicrobial resistance in Listeria spp. In: Chin-Yi Chen, Xianghe Yan, and Charlene Jackson (Eds.). Antimicrobial Resistance and Food Safety: Methods and Techniques. Academic Press/Elsevier Science, Amsterdam, The Netherlands. pp. 137-153.
  • Type: Book Chapters Status: Published Year Published: 2015 Citation: Ricke, S.C., T.M. Dawoud, and Y.M. Kwon. 2015. Chapter 4. Application of molecular methods for traceability of foodborne pathogens in food safety systems. In: S.C. Ricke, J.R. Donaldson, and C.A. Phillips (Eds.) Food Safety: Emerging Issues, Technologies and Systems. Elsevier, Oxford, UK, pp. 37-63.
  • Type: Book Chapters Status: Published Year Published: 2015 Citation: Handley, J.A., S.H. Park, Z. Shi, T.M. Dawoud, Y.M. Kwon, and S.C. Ricke. 2015. Chapter 6. Salmonella and the potential role for microbial process indicators on chicken carcasses. In: S.C. Ricke, J.R. Donaldson, and C.A. Phillips (Eds.) Food Safety: Emerging Issues, Technologies and Systems. Elsevier, Oxford, UK, pp. 81-104.
  • Type: Book Chapters Status: Published Year Published: 2015 Citation: Ricke, S.C., J. Rivera Calo, and P. Kaldhone. 2015. Chapter 7. Salmonella control in food production  Current issues and perspectives in the United States. In: S.C. Ricke, J.R. Donaldson, and C.A. Phillips (Eds.) Food Safety: Emerging Issues, Technologies and Systems. Elsevier, Oxford, UK, pp. 107-133.
  • Type: Book Chapters Status: Published Year Published: 2015 Citation: Ricke, S.C. 2015. Chapter 19. Application of anaerobic microbiology laboratory training and writing comprehension to food safety education. In: S.C. Ricke, J.R. Donaldson, and C.A. Phillips (Eds.) Food Safety: Emerging Issues, Technologies and Systems. Elsevier, Oxford, UK, pp. 395-419.
  • Type: Book Chapters Status: Published Year Published: 2015 Citation: Hanning, I., J. Lingbeck, and S.C. Ricke. 2015. Cardiovascular health and disease prevention: association with foodborne pathogens and potential benefits of probiotics. In: Bioactive Foods in Promoting Health: Probiotics-Prebiotics, 2nd Edition. R.R. Watson and V.R. Preedy (Eds.) Elsevier, San Diego, CA. pp. 793-806.
  • Type: Books Status: Published Year Published: 2015 Citation: Ricke, S.C., J.R. Donaldson, and C.A. Phillips (Eds.) 2015. Food Safety: Emerging Issues, Technologies and Systems. Academic Press, Elsevier, Inc., Oxford, UK, 449 pp.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2015 Citation: Roto, S.M., S.H. Park, Z. Shi, S.I., Lee, and S.C. Ricke. 2015. Broiler GIT microbiome response to diet containing Original-XPC and maturity analyzed via an Illumina MiSeq platform. 7th Annual Arkansas Association for Food Protection Educational Conference - Current Issues in Food Safety, Chancellor Hotel, Fayetteville, AR, Sept. 8-10.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2015 Citation: Lee, S.I., S.H. Park, and S.C. Ricke. 2015. Microbial population assessment of a commercial yeast-based prebiotic fed broiler chickens via an Illumina MiSeq platform and bird performance comparison. 7th Annual Arkansas Association for Food Protection Educational Conference - Current Issues in Food Safety, Chancellor Hotel, Fayetteville, AR, Sept. 8-10.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2015 Citation: Kaldhone, P.R., P.M. Rubinelli, C.A. Baker, and S.C. Ricke. 2015. Quantification of short chain fatty acids produced by Salmonella under anaerobic condition. 7th Annual Arkansas Association for Food Protection Educational Conference - Current Issues in Food Safety, Chancellor Hotel, Fayetteville, AR, Sept. 8-10.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2015 Citation: Roto, S.*, S.I. Lee, P. Rubinelli, S. H. Park, and S. Ricke. 2015. Cecal Salmonella and indigenous microflora reponse to original XPCTM-fed broilers. International Poultry Scientific Forum, Atlanta, GA, Jan. 26-27th.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2015 Citation: Roto, S.*, C. Baker, P. Kaldone, M. Miller, and S. Ricke. 2015. Evaluation of two antibody sera against Salmonella serovars that exhibit a broad range of surface antigens via flow cytometry. International Poultry Scientific Forum, Atlanta, GA, Jan. 26-27th.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2015 Citation: Park, S.H., F.G. Carbonero, and S.C. Ricke. 2015. Evaluation of microbial populations in chicken ceca raised on pasture flock fed with commercial prebiotics via sequencing (Illumina MiSeq). International Assoc. of Food Protection Annual Meeting, Portland, OR.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2015 Citation: Roto, S.*, S.H. Park, and S.C. Ricke. 2015. Analysis of the gut microbiome response of broilers fed with Original-XPCTM and salinomycin via an Illumina MiSeq platform. Poultry Science Association Annual Meeting, Louisville, KY, July 27-29th.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2015 Citation: Rubinelli, P.M., S.I. Lee*, S.H. Park, D. Korver and S.C. Ricke. 2015. Impact of different prebiotics on a Salmonella Enteritidis marker strain in rooster in vitro cecal incubations and on in vivo microbiota from layer hen cecal contents. Poultry Science Association Annual Meeting, Louisville, KY, July 27-29th.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2015 Citation: Rubinelli, P.M. and S.C. Ricke. 2015. Effect of sodium bisulfate and a sodium bisulfate/tannin mixture on Salmonella Typhimurium marker strain survival in adapted and unadapted anaerobic in vitro mixed cultures. Poultry Science Association Annual Meeting, Louisville, KY, July 27-29th.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2015 Citation: Rubinelli, P.M. and S.C. Ricke. 2015. Original XPCTM decreases Salmonella Typhimurium and Enteritidis populations and alters fermentation acid profiles in chicken in vitro cecal incubations. Poultry Science Association Annual Meeting, Louisville, KY, July 27-29th.


Progress 10/01/13 to 09/30/14

Outputs
Target Audience: Poultry and food commodity groups, poultry growers, poultry processors Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? Postdocs and graduate students intiated training on molcular techniques including microbiome analysis How have the results been disseminated to communities of interest? Twenty threetalks were given to wide range of audiences, locally, nationally and in international forums. What do you plan to do during the next reporting period to accomplish the goals? Continue research progress

Impacts
What was accomplished under these goals? 1) Salmonella virulence: Salmonella enterica with 1500 serovars has been considered as one of the most prominent foodborne pathogens in poultry industry. In last several years, the incidence of Salmonella Heidelberg was increased significantly in the USA and identified as one of the primary serovars associated with human illness. Although S. Heidelberg exhibits similar antigenic characteristics with S. Typhimurium one of the more common serovars in human diseases, S. Heidelberg has not been investigated nearly to the same extent. The aims of this research were to develop S. Heidelberg mutant library using transposon mutagenesis and identify essential genes for survival and virulence that could be used a potential targets for more effective intervention methods. Salmonella Heidelberg ARI-14 isolated from poultry was evaluated for ampicillin and kanamycin susceptibility prior to transposon mutant library construction because the EZ-Tn5 pMOD-6 plasmid DNA possesses both antibiotics genes as selection markers. The EZ-Tn5 pMOD-6 plasmid DNA was transformed to S. Heidelberg strain in advance to improve efficiency through host adaptation followed by development of a transposon mutant library. Salmonella Heidelberg ARI-14 strain was susceptible ampicillin (100 ug/ml) and kanamycin (60 ug/ml). The efficiency of EZ-Tn5 pMOD-6 plasmid DNA transformation to ARI-14 for host adaptation was 106 cells per ml. The excised transposon from host-adapted plasmid DNA was utilized for mutant library. The developed S. Heidelberg mutant library can be applied to various growth conditions like low pH and thermal stress to screen essential genes for survival and virulence. Identification of these genes will help to elucidate more effective control measures in food systems. 2) Salmonella metabolism: We hypothesized that growth responses will vary among Salmonella serovars grown in selective and nonselective growth media, and non-selective enrichment will be more efficient than selective enrichment for quantifying Salmonella serovars. In this study, we compared the non-selective enrichment medium Luria-Bertani (LB) to a selective-enrichment media (Tetrathionate (TT)) currently recommended by the USDA. One strain from each of the four most common poultry-associated Salmonella serovars (Typhimurium, Enteritidis, Heidelberg, and Kentucky) was tested. Approximately 100 colony forming units (CFU) of each strain were subsequently sub cultured in 100 mL of LB or TT broth, and incubated at 42°C for 24 h. At 0, 2, 4, 6, 8, 12, and 24 h, subsamples of each medium were diluted and plated in duplicate on LB plates to determine CFU per mL. Among all serovars, there was a 2.07 ± 0.65 log increase in CFU/mL at 12 h, and a 3.33 ± 1.90 log increase in CFU/mL at 24 h of growth in TT. However, there was a 2.24 ± 0.46 log increase in CFU/mL at 4 h, a 6.97 ± 0.25 log increase in CFU/mL at 12 h, and a 7.70 ± 0.43 log increase in CFU/mL at 24 h. Determining the growth responses of various Salmonella serovars will be useful to not only improve enrichment and culture methodology, but also reduce the time it takes to quantify Salmonella with rapid nonculture-based detection methods. 3) Prebiotics and gut microflora: Previous studies have demonstrated that various substances containing complex carbohydrates, such as yeast extracts and rice bran, can have an inhibitory effect on pathogenic bacteria, such as Salmonella, by a so-called “prebiotic” effect. Prebiotics are believed to change the ecology of the hindgut microflora in ways that preclude colonization of the gut by pathogens. We have evaluated bran from different rice cultivars for a prebiotic effect, using an anaerobic in vitro system that combines prebiotics with microflora from the cecum (hindgut) of chickens, for inhibition of Salmonella. The ceca of chickens at 6 weeks of age were removed and placed in an anaerobic chamber. A 0.1 g portion of the cecal contents was diluted and placed in culture bottles with ground chicken feed with or without addition of rice bran at a concentration of 1% w/v. As a positive control,a known prebiotic (a yeast extract) was tested in parallel. Salmonella serovars were then added to each bottle, and the mixed cultures were grown anaerobically at 37 oC with shaking. These cultures were then evaluated for Salmonella survival at 0, 24, and 48 hours after addition of Salmonella. One of the three rice brans tested exhibited a rapid inhibition of Salmonella, which became undetectable after 24 h of culture time, exceeding the inhibition by the known prebiotic. Salmonella was still undetectable in this rice bran at 48 h, while theknown prebiotictreatment had low (50 CFU/ml) but detectable Salmonella. Short chain fatty acid (SCFA) analysis of supernatant from these mixed cultures indicated a ten-fold reduction in both propionic and butyric acid concentrations in the inhibitory rice bran compared to the negative control culture. These results contrasted with the effects ofknown prebioticon SCFA, indicating the development of distinct ecological interactions with Salmonella in response to these prebiotics.

Publications

  • Type: Journal Articles Status: Published Year Published: 2014 Citation: Lingbeck, J.M., P. Cordero, C.A OBryan, M.G Johnson, S.C. Ricke, and Park SH, Gibson KE, Almeida G, Ricke SC (2014) Assessment of Gastrointestinal Microflora in Pasture Raised Chickens Fed Two Commercial Prebiotics. J Prob Health 2: 122. doi: 10.4172/2329-8901.1000122
  • Type: Journal Articles Status: Published Year Published: 2014 Citation: Dawoud, T., T. Jiang, S.C. Ricke, and Y.M. Kwon. 2014. Improving the efficiency of transposon mutagenesis in Salmonella Enteritidis by overcoming host-restriction barriers. Molecular Biotechnol. 56: 10041010.
  • Type: Journal Articles Status: Published Year Published: 2014 Citation: Park, S.H., M. Aydin, A.Khatiwara, M.C. Dolan, D.F. Gilmore, J.L. Bouldin, S. Ahn, and S.C. Ricke. 2014. Application of current and emerging rapid detection methodologies for identification of Salmonella spp. in poultry and poultry products. Food Microbiol. 38: 250-262.
  • Type: Journal Articles Status: Published Year Published: 2014 Citation: Saengkerdsub, S. and S.C. Ricke. 2014. Ecology and characteristics of methanogenic archaea in animals and humans. Crit. Revs. Microbiol. 40: 97116.
  • Type: Journal Articles Status: Published Year Published: 2014 Citation: OBryan, C.A., P.G. Crandall, Davis, M.L., G. Kostadini, K. E. Gibson, W. Q. Alali, D. Jaroni, S.C. Ricke, and J.A. Marcy. 2014. Mobile poultry processing units: a safe and cost-effective poultry processing option for the small-scale farmer in the United States. Worlds J. Poultry Sci. 70: 787-802.
  • Type: Journal Articles Status: Published Year Published: 2014 Citation: Golbach, J.L., S.C. Ricke, C.A. OBryan and P. G. Crandall. 2014. Riboflavin in nutrition, food processing, and analysis - A review. J. Food Res. 3: 23-35.
  • Type: Journal Articles Status: Published Year Published: 2014 Citation: Ricke, S.C. 2014. Application of molecular approaches for understanding foodborne Salmonella establishment in poultry production. Advances in Biology, vol. 2014, Article ID 813275, 25 pages, 2014. doi:10.1155/2014/813275
  • Type: Conference Papers and Presentations Status: Published Year Published: 2014 Citation: Park, S.H. and S.C. Ricke. 2014. Construction of Salmonella Kentucky mutant library using a transposon mutagenesis system. South Central American Society Branch Meeting, Fayetteville, AR, Sept. 12-13.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2014 Citation: Baker, C., S.H. Park, J.R. Calo, and S.C. Ricke. 2014. Comparison of Salmonella serovar growth response in selective and non-selective enrichment media. South Central American Society Branch Meeting, Fayetteville, AR, Sept. 12-13.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2014 Citation: ) Rubinelli, P. and S.C. Ricke. 2014. Evaluation of bran from different rice cultivars as inhibitors of Salmonella in an in vitro mixed culture system with chicken hindgut microflora. South Central American Society Branch Meeting, Fayetteville, AR, Sept. 12-13.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2014 Citation: Lee, S.I., S. H. Park and S.C. Ricke. 2014. Assessment of gastrointestinal microflora shifts and investigation of integron class 1 gene prevalence in conventional flock raised chickens fed with commercial prebiotics. 6th Annual Arkansas Association for Food Protection Educational Conference - Exploration of Emerging Issues in Food Safety, Chancellor Hotel, Fayetteville, AR, Sept. 11-13.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2014 Citation: Roto, S., A. Baker, M. Miller, and S.C. Ricke. 2014. Evaluation of two antibody sera against recent Salmonella enterica outbreak serovars with flow cytometry. 6th Annual Arkansas Association for Food Protection Educational Conference - Exploration of Emerging Issues in Food Safety, Chancellor Hotel, Fayetteville, AR, Sept. 11-13.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2014 Citation: Roto, S., S. I. Lee, S. H. Park, and S.C. Ricke. 2014. Investigation of microflora population and Salmonella prevalence isolated from ceca of chickens fed XPC. 6th Annual Arkansas Association for Food Protection Educational Conference - Exploration of Emerging Issues in Food Safety, Chancellor Hotel, Fayetteville, AR, Sept. 11-13.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2014 Citation: Park, S.H., T. Dawoud, Y. M. Kwon and S.C. Ricke. 2014. Development of Salmonella Heidelberg mutant library using a transposon mutagenesis system. International Assoc. Food Prot. Annual Meeting, Indianapolis, IN Aug. 3-6.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2014 Citation: Lee, S.I, S.H. Park, and S.C. Ricke. 2014. Assessment of gastrointestinal microflora shifts in conventional flock raised chickens fed with commercial prebiotics. Poultry Science Association Annual Meeting, Corpus Christi, TX July, 14-17.


Progress 10/01/12 to 09/30/13

Outputs
Target Audience: Poultry and food commodity groups, poultry growers, poultry processors Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? Nothing Reported How have the results been disseminated to communities of interest? Presented invitedtalks to a wide range of audeinces both nationally and internationally. What do you plan to do during the next reporting period to accomplish the goals? Continue research projects

Impacts
What was accomplished under these goals? 7peer refereed research and reviewarticles; 19 proceedings and symposia; 21 invited talks; 7 published abstracts

Publications

  • Type: Journal Articles Status: Published Year Published: 2013 Citation: 1) Alali, W.Q., C. L. Hofacre, G. F. Mathis, G. Faltys, S. C. Ricke, and M. P. Doyle. 2013. Effect of non-pharmaceutical compounds on shedding and colonization of Salmonella enterica serovar Heidelberg in broilers. Food Control 31: 125-128. 2) Park, S.H., D. Biswas, J. Lingbeck, O. K. Koo and S.C. Ricke. 2013. Enhancement of macrophage chicken cytokine response to Salmonella Typhimurium when combined with bacteriophage P22. FEMS Microbiol. Letts. 339: 137144. 3) Trimble, L.M., W. Q. Alali, K.E. Gibson, S. C. Ricke, P. Crandall, D. Jaroni, and M. Berrang. 2013. Salmonella and Campylobacter prevalence and concentration on pasture-raised broilers processed on-farm, in a mobile processing unit, and at small USDA-inspected facilities. Food Control 34: 177-182. 4) Trimble, L.M., W. Q. Alali, K.E. Gibson, S. C. Ricke, P. Crandall, D. Jaroni, M. Berrang and M.Y. Habteselassie. 2013. Salmonella and Campylobacter in the processing environment of small-scale pastured broiler farms. Poultry Sci. 92: 30603066. 5) Ricke, S.C., C.S. Dunkley, and J.A. Durant. 2013. A review on development of novel strategies for controlling Salmonella Enteritidis colonization in laying hens: Fiber  based molt diets. Poultry Sci. 92: 502525. 6) Ricke, S.C., Khatiwara, A., and Y.M. Kwon. 2013. Application of microarray analysis of foodborne Salmonella in poultry production: A review. Poultry Sci. 92: 22432250. 7) Foley, S.L., T.J. Johnson, S.C. Ricke, R. Nayak and J. Danzeisen. 2013. Salmonella pathogenicity and host adaptation in chicken-associated serovars. Microbiol. and Mol. Biol. Revs. 77: 582-607.