Source: UNIVERSITY OF MAINE submitted to
FACTORS AFFECTING ORGANIC MATTER DECOMPOSITION AND STABILIZATION IN SOILS.
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
COMPLETE
Funding Source
Reporting Frequency
Annual
Accession No.
0229942
Grant No.
(N/A)
Cumulative Award Amt.
(N/A)
Proposal No.
(N/A)
Multistate No.
(N/A)
Project Start Date
Oct 1, 2012
Project End Date
Sep 30, 2017
Grant Year
(N/A)
Program Code
[(N/A)]- (N/A)
Recipient Organization
UNIVERSITY OF MAINE
(N/A)
ORONO,ME 04469
Performing Department
Plant, Soil and Environmental Sciences
Non Technical Summary
Because soil organic matter stores both nutrients and water, it is a key to soil quality in both agricultural and forested ecosystems. The overall goal of this proposal is to help us understand the factors that preserve organic matter in soils and also understand how soil organic matter levels may be affected by rising temperatures over the coming decades. Many studies suggest that soil organic matter may be lost in areas that are warming due to climate change because microbial decomposition of organic matter is generally more rapid as temperature increases. This basic information about ecosystem processes is also important for agricultural systems, particularly organic and sustainable systems which depend on building levels of soil organic matter to supply nutrients and increase water holding capacity. Compost is an organic amendment often used by organic and sustainable producers to build soil quality and, sometimes, to control pests. More information about different types of compost will allow more effective use of compost. Maine has appropriate compost feedstocks, such as seafood waste, wood waste, and animal manure, for producing high quality compost for organic and sustainable agricultural producers. Research on such composts may allow producers to develop high value composts targeted toward specific goals of nutrient management or pest control. A number of laboratory incubation studies will be conducted over several weeks or months. Materials to be incubated at a range of temperatures include: soluble and sorbed organic matter extracted from composts and plant residues, and compost amended and unamended soils. Composts produced by a number of commercial producers in Maine using a variety of feedstocks and production methods will be included in the incubation studies. Soil microbial and chemical properties will be determined periodically during the incubations. Information generated by this project will cause a change in knowledge by scientists interested in the response of soil organic matter to warming conditions. Also the information generated by this project may cause a change in knowledge and action by producers of commercial compost, as well as by organic growers and gardeners. Anticipated outcomes from this project are increased knowledge of the decomposition characteristics and chemical composition of a range of composts produced commercially in Maine. From this knowledge it may be possible to design strategies to produce compost which suppresses pests. It may also be possible to produce composts which balance maturity with the ability to optimally stimulate soil microbial activity.
Animal Health Component
(N/A)
Research Effort Categories
Basic
(N/A)
Applied
(N/A)
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
1020110200050%
4030110200050%
Goals / Objectives
The project objectives are: 1. Determine the decomposition kinetics of soluble and sorbed organic matter in soils as a function of temperature. 2. Determine the influence of compost chemistry and composition on rate of compost decomposition. 3. Determine the influence of soil amendments on soil chemistry and quality. The project outputs include: experiments conducted; results produced, analyzed, and synthesized; and knowledge to be discussed at conferences, meetings, and workshops.
Project Methods
The concentration of total dissolved organic carbon in the water extracts of soil and compost will be determined using a Shimadzu TOC 5000 analyzer. Fluorescence EEM/PARAFAC characterization of the extracted organic matter will be conducted with a Hitachi F-4500 spectrofluorometer. Microbial decomposition of the soluble organic matter will be conducted using a short term laboratory incubation. Solutions containing both fresh and microbially-decomposed soluble organic matter will be used to evaluate the extent of soluble organic matter sorption onto synthetic goethite. After sorption, the goethite plus sorbed organic matter will be incubated at a range of temperatures to provide the decomposition kinetics of sorbed organic matter. The identical quantity of soluble (non-sorbed) carbon will be incubated at the same range of temperatures to provide the decomposition kinetics of the soluble organic matter. The temperature sensitivity of the decomposition of both the labile and stabile pools will be assessed by using the Arrhenius equation. Composts produced at different facilities using a variety of feedstocks, will be collected, dried and ground, and analyzed by standard methods. Two simultaneous incubation experiments will be conducted using compos-amended soils and an unamended control soil, a continuous carbon mineralization assay and an incubation to be destructively sampled for water stable aggregates, pH, extractable carbon and nitrogen, and microbial biomass. For both of the incubation experiments, field-moist soil will be pre-incubated at 25 C for several days, then mixed with dried and ground composts (5g C/kg soil). For the carbon mineralization assay, there will be three replicates housed in an incubator at 25 C. At appropriate sampling intervals (e.g. days 1, 3, 7, 11, 15, and thereafter at intervals of 2-3 weeks for several months) gas samples will be withdrawn from the vials and analyzed for CO2 using a Li-Cor LI-7000 CO2 Analyzer. Carbon mineralization of amended and unamended soils will be measured at 10, 15, 20, and 30 C for 60 days for a subset of the composts using two replications. A second set of compost-treated soils with an unamended control will be incubated at 25 C. These soils will be destructively sampled on approximately days 0, 3, 7, 14, 21, 35, 59, and 118. A 50 g subsample will be air-dried for future water stable aggregate analysis using an Eijkelkamp wet sieving apparatus, using 1 mm and 0.25 mm screen sizes, to determine aggregate stability. The remaining soil will be stored at 4 C until analysis for microbial biomass and pH. Water extractable organic carbon will be collected and its concentration and fluorescence spectra will be determined as previously described. Inorganic nitrogen will be extracted with 1 N KCl and quantified using a O.I. Alpkem A/E analyzer. Data will be analyzed by ANOVA. Fishers least significant difference will be calculated as appropriate when a treatment effect is indicated by the ANOVA. Information will be published in scientific journals and discussed at local and regional meetings of stakeholders.

Progress 10/01/12 to 09/30/17

Outputs
Target Audience: Nothing Reported Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided?One graduate student working on this project received an M.S. degree. Another student's M.S. degree is in progress. How have the results been disseminated to communities of interest?We presented results at a recent professional meeting: Audrey Laffely, Susan Erich, and Ellen Mallory Evaluating Root Effects on the CO2 Flush from Laboratory Dried and Rewetted Soils. Soil Science Society of America meeting, Tampa, FL. Oct. 25, 2017. Audrey Laffely (graduate student) also gave a presentation on her project to the public at the Roger Farm field day, Jully 6, 2017. What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? High yield agricultural systems, such as high tunnel (HT) vegetable production, require a large supply of soil nutrients, especially nitrogen (N). Compost is a common amendment used by HT growers both to supply nutrients and to improve physical and biological soil properties. We examined commercially-available composts and their effects on soil N, plant N uptake, and tomato yield in HT cultivation. In addition, a laboratory study examined N and carbon (C) mineralization from the composts, and the usefulness of compost properties as predictors of compost N mineralization was assessed under field and laboratory conditions. The field study used a randomized complete block design with four replications to compare four compost treatments (all added at the rate of 300 kg total N ha-1 ) with unamended soil and an inorganic N treatment (110 kg N ha-1 ). Tomatoes were grown in Monmouth, ME during the summers of 2013 and 2014. Compost NO3--N and NH4+-N application rates were significantly correlated with soil NO3--N and NH4+-N concentrations throughout the growing season. Marketable yield was positively correlated with compost total inorganic N and NO3--N in both years, and with NH4+-N in 2014. There were no significant differences among composts in percentage of organic N mineralized and no correlations were observed with any measured compost property. In the laboratory study, all compost-amended soils had relatively high rates of CO2 release for the initial few days and then the rates declined. The compost-amended soils mineralized 4-6% of the compost organic N. This study suggested compost inorganic N content controls N availability to plants in the first year after compost application. The CO2 flush is the amount of CO2 released from laboratory dried and rewetted soil; it has been used as a rapid, inexpensive soil quality test. We examined the effects of root growth on the CO2 flush in a field experiment. We found that although the CO2 flush was significantly higher from planted plots than from bare plots, the effect was relatively minor. LiCOR results were similar to the commercial CO2 flush test (Solvita, Woods End Laboratory, Mt. Vernon, ME). Planted plots had higher soluble organic C and higher microbial biomass compared to bare plots.

Publications

  • Type: Conference Papers and Presentations Status: Published Year Published: 2017 Citation: Audrey Laffely, Susan Erich, and Ellen Mallory Evaluating Root Effects on the CO2 Flush from Laboratory Dried and Rewetted Soils. Soil Science Society of America meeting, Tampa, FL. Oct. 25, 2017


Progress 10/01/15 to 09/30/16

Outputs
Target Audience: Nothing Reported Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? Nothing Reported How have the results been disseminated to communities of interest?Through journal articles and presentations at grower meetings and professional meetings What do you plan to do during the next reporting period to accomplish the goals?A new graduate student is planning her thesis project around the goals of the project.

Impacts
What was accomplished under these goals? High yield agricultural systems, such as high tunnel (HT) vegetable production, require a large supply of soil nutrients, especially nitrogen (N). Compost is a common amendment used by HT growers both to supply nutrients and to improve physical and biological soil properties. We examined commercially-available composts and their effects on soil N, plant N uptake, and tomato yield in HT cultivation. In addition, a laboratory study examined N and carbon (C) mineralization from the composts, and the usefulness of compost properties as predictors of compost N mineralization was assessed under field and laboratory conditions. The field study used a randomized complete block design with four replications to compare four compost treatments (all added at the rate of 300 kg total N ha-1 ) with unamended soil and an inorganic N treatment (110 kg N ha-1 ). Tomatoes were grown in Monmouth, ME during the summers of 2013 and 2014. Compost NO3--N and NH4+-N application rates were significantly correlated with soil NO3--N and NH4+-N concentrations throughout the growing season. Marketable yield was positively correlated with compost total inorganic N and NO3--N in both years, and with NH4+-N in 2014. There were no significant differences among composts in percentage of organic N mineralized and no correlations were observed with any measured compost property. In the laboratory study, all compost-amended soils had relatively high rates of CO2 release for the initial few days and then the rates declined. The compost-amended soils mineralized 4-6% of the compost organic N. This study suggested compost inorganic N content controls N availability to plants in the first year after compost application.

Publications

  • Type: Journal Articles Status: Published Year Published: 2016 Citation: Marshall, K.; Erich, M.S.; Hutton, M.; Hutchinson, M.; Mallory, E.B. 2016. Nitrogen availability from compost in high tunnel tomato production. Compost Sci. Util. 24:147-158.


Progress 10/01/14 to 09/30/15

Outputs
Target Audience: Nothing Reported Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? Nothing Reported How have the results been disseminated to communities of interest?Through a peer-reviewed journal article. What do you plan to do during the next reporting period to accomplish the goals?Another peer-reviewed journal article plus a presentation to the New England Fruit and Vegetable Conference in NH in December 2015.

Impacts
What was accomplished under these goals? The Araucaria moist forest was once widespread in southern Atlantic Brazil but today, due to agriculture and timber harvesting, only remnants remain, mainly in mountainous regions of southern Brazil. This study combines Hedley sequential extraction to determine phosphorus (P) fractionation with 31P NMR, fluorescence spectroscopy, and ESI-FT-ICR-MS to examine soil P and organic matter chemistry at five different Araucaria moist forest sites, including two natural sites, grassland and native forest, and three altered sites, two apple orchard sites (fertilized apple rows and unfertilized soil between apple rows) and a pine plantation site without fertilization. This study examined how land use change has altered soil chemistry compared to native vegetation, focusing specifically on P and C fractions. Although the native grassland site contained the highest amount of soil C, the sites were similar in total P. The largest extractable fraction at all sites was NaOH-extractable organic P (Po), and the quantity of Po (0.1M plus 0.5M extractions) did not differ significantly among the soils. The31P NMR analysis revealed the majority of NaOH-extractable P was present as either inorganic P (Pi) orthophosphate (49%) or Po monoesters (34%). Pi orthophosphate was highest in pine plantation and apple orchard in the row sites (66 and 67% of P detected, respectively) and lowest in native grassland (20% of P detected), while Po was highest in the native grassland soil (75% of P detected). In our samples ~ 1% or fewer of labile C compounds contained P, while 5-6% of adsorbed C compounds contained both N and P. Using 31P NMR and ESI-FT-ICR-MS approaches, we found evidence of chemical changes in native soil P and organic matter to due land use change, specifically loss of organic forms of P and increased organic matter aromaticity in apple soils compared to native soils. This research shows that the conversion of native grasslands to apple orchards led to a decrease in organic matter quality and Po depletion while conversion to pine plantations has a low impact on these parameters. These analytical techniques are promising approaches to improving our understanding of both P and C chemistry and the effects of land use changes on coupled biogeochemical cycles.

Publications

  • Type: Journal Articles Status: Published Year Published: 2015 Citation: Boeira de Oliveira , C.M., Erich, M.S., Gatiboni, L.C., Ohno, T. 2015. Phosphorus fractions and organic matter chemistry under different land use on Humic Cambisols in Southern Brazil. Geoderma Regional 5:140-149.


Progress 10/01/13 to 09/30/14

Outputs
Target Audience: Two presentations were given at the 2014 Maine Vegetable and Fruit School, March 10 and 11, 2014 in Portland and Bangor Maine on compost use as a soil amendment to supply nitrogen. The audience was primarily growers, scientists, and students. A poster was presented at the annual American Society for Horticultural Sciences meeting in July, 2014. The audience was primarily scientists and students. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? One graduate student is working on this project How have the results been disseminated to communities of interest? Presentations were given at the Maine Vegetable and Fruit School in Portland and Bangor, Maine and at the American Societyof Horticultural Science meeting in Orlando, FL. What do you plan to do during the next reporting period to accomplish the goals? A publication will be submitted in the next reporting period.

Impacts
What was accomplished under these goals? We conducted an experiment examing four finished composts from commercial producers in Maine, including their properties and how they performed in a field study. The composts were analyzed for nutrient content, pH, conductivity, lignin, and soluble C. A randomized complete block design with four replications was used to compare the four compost treatments with an unamended control and a control receiving inorganic N in a field study. Composts were all added at the rate of 270 lb. of total N acre-1, while inorganic fertilizer was added at the rate of 100 lb. N acre-1. Tomatoes were grown in these treatments in caterpillar tunnels at the Maine Agricultural and Forest Experiment Station Farm in Monmouth, ME during the summers of 2013 and 2014. Soil nitrate (NO3- ), ammonium (NH4+), and moisture were measured throughout the growing season, along with total marketable tomato yield and tomato N uptake at the end of the season. Composts had significantly different properties. In 2013 compost treatments affected early season soil NO3- and NH4+, and total marketable yield, and composts with high levels of inorganic N produced higher total marketable yields. Results from 2013 were reported at the American Society for Horticultural Sciences annual meeting Orlando, FL. The citation for this presentation is: Marshall, K., S. Erich, M. Hutton, M. Hutchinson, E. Mallory. 2014 Compost for High Tunnel Tomato Production. (Abstract) https://ashs.confex.com/ashs/2014/webprogram/Paper19760.html

Publications


    Progress 10/01/12 to 09/30/13

    Outputs
    Target Audience: Because it is early in the project, we do not yet have many results to report. My graduate student reported on her study of compost as a soil amendment for tomatoes grown in high tunnels at the Highmoor Farm Field day at Highmoor Farm, Monmouth, Maine on July 31, 2013. The audience included scientists, producers, and staff from state and private agencies. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? I have one graduate student working on a project on the effects of four different composts on yield and nitrogen content of tomato plants grown in high tunnels. She developed her thesis proposal, conducted one summer of field research, and is now analyzing her data. She is also planning a laboratory incubation to look at compost decomposition and nitrogen release. How have the results been disseminated to communities of interest? Nothing Reported What do you plan to do during the next reporting period to accomplish the goals? My graduate student will carry out a laboratory incubation and conduct another summer of field research.

    Impacts
    What was accomplished under these goals? I was sabbatic leave in the spring semester of 2013, spending 10 weeks at the Institute of Agro-Environmental Sciences in Tsukuba, Japan, where I conducted a short term experiment examining the chemistry and biodegradation of extractable C. Using two soils (tilled and no-till) and two incubation temperatures (15 and 35), both water- and pyrophosphate-extractable C were extracted at 4 times of incubation. The following properties were measured: pH, uv absorbance, ICP [Al, Fe, Ca, Mg, K] , NPOC, total N, and biodegradability. Data is being analyzed and interpreted.

    Publications