Progress 08/01/12 to 07/31/14
Outputs
Target Audience: Progress and results of this project have been disseminated to both scientific and stakeholder audiences. Since the last reporting period I have given seminars discussing population genetic structuring of onion thrips in relation to economically important traits at UC Davis, Cornell, University of Hawaii, Auburn University, University of Thessaloniki, Greece, and Monsanto. I have also given a talk to NY onion growers at the NY Empire Expo and published an article in Onion World Magazine about the implications of this project on insecticide resistance management in NY muck onion agroecosystems. This Fall I will be presenting results at the National meeting of the Entomological Society of America, and have been invited to give a talk at the International Symposium of Thrips and Tospoviruses meeting next spring. Changes/Problems: No major changes were made to the project. The only challenge encountered was an unexpected result suggesting that ongoing gene flow occurs between two of the previously characterized lineages of onion thrips. To explain our results we needed a laboratory study to provide validation that this is possible. Luckily, a Masters student examining the potential for gene flow between these two groups generated data that corroborated with our results. Currently, we are awaiting publication of those results so that we can proceed with publishing our manuscript. What opportunities for training and professional development has the project provided? This project has allowed me to develop technical and analytical skills needed to use population genetics approaches to inform insect pest management decisions. During this project I have also had the opportunity to practice and refine oral and written communication skills, network, and develop two additional collaborations to further assess related questions in NY and Greek agroecosystems. How have the results been disseminated to communities of interest? The results and applications of these studies have also been disseminated outside of the scientific community. I have given a talk to NY onion growers at the NY Empire Expo and published an article in Onion World Magazine. I also plan to assist Brian Nault at Cornell with preparation of any additional educational materials that may include content from this study. What do you plan to do during the next reporting period to accomplish the goals?
Nothing Reported
Impacts
What was accomplished under these goals?
The genetic analyses needed to address these objectives were completed. Population genetic structuring of NY onion thrips were evaluated in relation to geographic distance, genetic distance, and reproductive mode. In 2011 and 2012 samples were collected early- and late-season to assess seasonal changes in population structuring. A manuscript is currently in preparation.
Publications
- Type:
Journal Articles
Status:
Other
Year Published:
2015
Citation:
Population genetic structure of arrhenotokous and thelytokous Thrips tabaci in New York onion cropping systems
- Type:
Journal Articles
Status:
Published
Year Published:
2014
Citation:
A.L. Jacobson, E.L. Vargo, G.G. Kennedy, and B.A. Nault. (2014) Onion thrips population genetics and implications for management. Onion World Magazine. February Issue.
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Progress 08/01/12 to 07/31/13
Outputs
Target Audience: Updates on the results of these studies have been shared with Ed Vargo's, George Kennedy's, and Brian Nault's lab groups at North Carolina State University and Cornell University. Research seminars have also been given at the University of Georgia, the University of Thessaloniki, Greece, and to PIs of the Thrips-Tospovirus CAP Grant. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided? This project has provided me the opportunity to work with new lab groups at North Carolina State University, and with researchers at Cornell University. It has also generated collaborations with researchers in Greece, where Thrips tabaci is an economically important pest, and primary vector of TSWV in tobacco production systems. Because of my experience working with thrips I was selected to participate in a mentoring program that is part of the Thrips-Tospovirus CAP grant. This grant will also be funding my attendance at the Annual Meeting of the Entomological Society of America this year, and will provide opportunities to attend more professional and grower meetings next year (see below). How have the results been disseminated to communities of interest? Updates on the results of these studies have been shared with Ed Vargo, George Kennedy, and Brian Nault lab groups at North Carolina State University and Cornell University. Currently the manuscript for objective 1 is being prepared for publication. What do you plan to do during the next reporting period to accomplish the goals? The microsatellite marker experiments, sequencing of mtCOI, and sequencing of sodium channel markers will be finished this fall. After the experiments have concluded the data will be analyzed and manuscripts prepared. After all of the analyses have been conducted I will also help to prepare extension materials with Brian Nault for NY onion producers. Next year I plan to give a talk at the NY Empire State EXPO, and if possible, a departmental seminar at Cornell. In addition, I am scheduled to give a departmental seminar at U.C. Davis, and plan to attend the International Thrips-Tospovirus Meeting to disseminate the results of my studies.
Impacts
What was accomplished under these goals?
Since beginning my postdoctoral fellowship I have completed the research for objective 1 of my USDA fellowship. I am currently preparing a manuscript to present the results of objective 1. Progress has also been made on objective 2. The microsatellite marker component for first year samples (of the 2-year sampling plan) have been run, and analyzed. The last year of thrips collections is also almost completed: early season thrips collection trips took place in late May 2013, and the late season collection trip will take place at the end of September. The second year’s thrips samples are currently being processed.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2013
Citation:
Manuscript from this project:
Thrips tabaci population genetic structure in relation to geographic location and reproductive forms in New York onion cropping systems: In preparation. Target Journal, Molecular Ecology.
Manuscript publication from Ph.D. (not funded by this grant):
A.L. Jacobson and G.G. Kennedy. (2013) Effect of cyantraniliprole on feeding behavior and virus transmission of Frankliniella fusca and Frankliniella occidentalis (Thysanoptera: Thripidae) on Capsicum annuum. Crop Protection. In Press.
A.L. Jacobson and G.G. Kennedy. (2013) Electrical penetration graph studies to investigate the effects of cyantraniliprole treatments on feeding behavior of Myzus persicae (Hemiptera: Aphididae) on Capsicum annuum. Pest Manag. Sci. In press
A.L. Jacobson and G.G. Kennedy. (2013) Specific insect-virus interactions are responsible for the vector competence of different Thrips tabaci isolines to transmit different Tomato spotted wilt virus isolates. PLoS One. 8(1): doi:10.1371/journal.pone.0054567
A.L. Jacobson, W. Booth, E.L. Vargo, and G.G. Kennedy. (2013) Thrips tabaci population genetic structure and polyploidy in North Carolina in relation to competency as a vector of Tomato spotted wilt virus. PLoS One. 8(1): doi:10.1371/journal.pone.0054484
A.L. Jacobson, J.S. Johnston, D. Rotenberg, A.E. Whitfield, W. Booth, E.L. Vargo and G.G. Kennedy. (2013) Genome size and ploidy inference in four species of Thysanoptera, Thripidae and Aeolothripidae. Insect Molecular Biology. 22(1): 12-17. doi:10.1111/j.1365-2583.2012.01165.x
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