Progress 03/19/12 to 09/30/16
Outputs
Target Audience:Researchers working in poultry health and vaccine manufacturers. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?We were able to train post doctoral fellow and graduate students on how to use recombiant technology generate novel candidate vaccines. How have the results been disseminated to communities of interest?The results were presented at an international conference. What do you plan to do during the next reporting period to accomplish the goals?If we get continued funding we will use the candidate vaccine to develop next generation vector vaccines capable of protecting multiple poultry diseases.
Impacts
What was accomplished under these goals?
Marek's disease is caused by a herpesvirus that causes a cancer like disease in chicken. The goal of this project was to develop safe and efficacious vaccines capable of protecting commercial chickens against Marek's disease. Earlier we have shown that deletion of the viral oncogene, Meq, results in superior vaccines, but such vaccines lacked safety. In this project we improved the safety of recombinant vaccines by gene deletion method. The resulting candidate vaccine was superior because it had improved safety profile without affecting protective efficacy against highly virulent field strains.
Publications
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2014
Citation:
Sun A, Khan O, Zhuang G, Lupiani B, and Reddy SM. Double deletion Mareks disease virus as a potential vaccine candidate. 10th International Symposium on Mareks Disease and Avian Herpesviruses. East Lansing MI, July 2014.
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Progress 10/01/13 to 09/30/14
Outputs
Target Audience:Vaccine manufacturers and academia Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?Trained post doctoral fellow in animal experimentation methodology. How have the results been disseminated to communities of interest?Presented the work in an international conference and talked to industry about potential for commercial use. What do you plan to do during the next reporting period to accomplish the goals?
Nothing Reported
Impacts
What was accomplished under these goals?
Marek's disease genes play a major role in pathogenesis. The objective of this grant to improve the safety of serotype 1 vaccines by deleting genes involved in replication. To this objective we have deleted two important viral genes from the virus to generate a safe and efficacious vaccine capable of protecting chickens against highly virulent field strains.
Publications
- Type:
Conference Papers and Presentations
Status:
Other
Year Published:
2014
Citation:
Sun A, Khan O, Zhuang G, Lupiani B, and Reddy SM. Double deletion Mareks disease virus as a potential vaccine candidate. 10th International Symposium on Mareks Disease and Avian Herpesviruses. East Lansing MI, July 2014.
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Progress 01/01/13 to 09/30/13
Outputs
Target Audience:Scientists, academia, poultry producers and vaccine manufacturers. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?Through this project we trained a postdoctoral fellow on development of MDV mutant viruses with potential use as vaccines. How have the results been disseminated to communities of interest?They have been published as scientific articles. What do you plan to do during the next reporting period to accomplish the goals?Plan to see if the products generated will protect against MD.
Impacts
What was accomplished under these goals?
Developed tools to introduce mutations into the genome of a highly virulent strain of Marek's disease virus. Using these tools we studied gene function of various viral genes. Specifically we showed the role ofribonucleotide reductase gene in pathogenesis.
Publications
- Type:
Journal Articles
Status:
Published
Year Published:
2013
Citation:
Reddy SM, Sun A, Khan OA, Lee LF and Lupiani B. 2013. Cloning of a very virulent plus, 686 strain of Mareks disease virus as a bacterial artificial chromosome. Avian Diseases 57 (2 Suppl): 469-473.
- Type:
Journal Articles
Status:
Published
Year Published:
2013
Citation:
Sun A, Lee LF, Khan OA, Heidari M, Zhang H, Lupiani B and Reddy SM. 2013. Deletion of Mareks disease virus large subunit of ribonucleotide reductase impairs virus growth in vitro and in vivo. Avian Diseases 57 (2 Suppl): 464-468.
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Progress 01/01/12 to 12/31/12
Outputs
OUTPUTS: Trained one post-doctoral fellow. The results were presented at a international conference so that researchers could utilize the information to developed control strategies to control economically important poultry diseases. Collaborations were established with other scientists working in the poultry disease field. Target audiences include researchers and poultry vaccine manufacturers. PARTICIPANTS: Individuals that worked on the project include Sanjay Reddy, Aijun Sun, Blanca Lupiani, Owais Khan Partnered with USDA ARS laboratory in East Lansing Michigan Collaborated with Lucy Lee Trained one post doctoral fellow. TARGET AUDIENCES: Target audiences include researchers and poultry vaccine manufacturers. PROJECT MODIFICATIONS: Not relevant to this project.
Impacts
Marek's disease (MD) is a lymphoproliferative and neuropathic disease of chickens, caused by a highly contagious, cell-associated, oncogenic herpesvirus called Mareks disease virus (MDV). Over the past 40 years, MD has been successfully controlled by the use of live virus vaccines. Vaccination protects against tumor development and mortality but does not provide sterilizing immunity. Thus, MDV continue to evolve towards greater virulence with concomitant reduction of vaccine efficacy. As a consequence, MD remains an economical important disease and the Animal Protection Program of AFRI considers MD research as high priority. Two independent BAC clones from the vv+ 686 strain of MDV-1. These cloned viruses were stably maintained in bacteria and are amenable for site directed mutagenesis. Pathogenesis studies showed that BAC derived viruses are highly oncogenic although they showed slightly reduced pathogenic phenotype compared to parental virus. These viruses were stable based on limited genomic analysis of the viral genome present in tumors. Even though a BAC clone from a hypervirulent MDV-1 that shares characteristics with vv+ strains has been recently reported to our knowledge this is the first report of the construction of a BAC clone from a vv+ MDV that was classified based on it ability to cause significant disease in birds vaccinated with bivalent vaccine (HVT+SB-1). The availability of these clones will aid in understanding the role played by individual MDV genes in pathogenesis. Using these BAC clones we deleted two important genes of MDV, preliminary results indicated that they are attenuated. We propose to further evaluate the efficacy of these clones to protect against highly virulent MDV field strains.
Publications
- Sun A., Lupiani B., Lee L.F., Reddy S.M. Cloning of a very virulent plus, 686 strain of Mareks disease virus as a bacterial artificial chromosome. 9th International Meeting on Mareks Disease and Avian Herpesviruses. Berlin, Germany June 2012
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