Progress 10/01/12 to 09/30/15
Outputs Target Audience:The major hypothesis of this project is that the induction of robust memory T cell populations using attenuated Listeria monocytogenes will protect not only be protetive against the development of encephalic listeriosis in cattle, but might be a powerful vaccine vector for the induction of T cell response agains antigens from other infectious agents or maligencies. Thus, during this funding period we have testing whether a novel, attenuated strain of L. monocytogenes can induced protective immunity against secondary L. monocytogenes challenge, as well as other infectious agents. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?
Nothing Reported
How have the results been disseminated to communities of interest?
Nothing Reported
What do you plan to do during the next reporting period to accomplish the goals?
Nothing Reported
Impacts What was accomplished under these goals?
We have demonstrated that the prs2A- htrA- L. monocytogenes strain is a highly attenuated mutant of Listeria monocytogenes. Even though it is highly attenuated it remains highly immunogenic and provide robust protective immunity against subsequent lethal L. monocytogenes challenge through the induction of strong Listeria-specific CD8+ and CD4+ T cell responses.
Publications
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Progress 10/01/13 to 09/30/14
Outputs Target Audience: One hypothesis of this project is that the induction of robust memory T cell populations using attenuated Listeria monocytogenes will protect against the development of encephalic listeriosis. Thus, during this funding period we have testing whether novel, attenuated strains of L. monocytogenes can induced protective immunity against L. monocytogenes infection. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?
Nothing Reported
How have the results been disseminated to communities of interest?
Nothing Reported
What do you plan to do during the next reporting period to accomplish the goals? We are currently writing a manuscript based on the finding of this chaperone deficient strain of L. monocytogenes is highly immunogenic and likely a good vaccine candidate.
Impacts What was accomplished under these goals?
We have recently demonstrated that the prs2A- htrA- L. monocytogenes strain is highly immunogenic and provide robust protective immunity against subsequent lethal L. monocytogenes challenge through the induction of strong Listeria-specific CD8+ and CD4+ T cell responses.
Publications
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Progress 01/01/13 to 09/30/13
Outputs Target Audience: One hypothesis of this project is that the induction of robust memory T cell populations using attenuated Listeria monocytogenes will protect against the development of encephalic listeriosis. Thus, during this funding period we have testing whether novel, attenuated strains of L. monocytogenes can induced protective immunity against L. monocytogenes infection. Changes/Problems:
Nothing Reported
What opportunities for training and professional development has the project provided?
Nothing Reported
How have the results been disseminated to communities of interest?
Nothing Reported
What do you plan to do during the next reporting period to accomplish the goals? We are currently writing a manuscript based on the finding of this chaperone deficient strain of L. monocytogenes is highly immunogenic and likely a good vaccine candidate.
Impacts What was accomplished under these goals?
We have recently demonstrated that the prs2A- htrA- L. monocytogenes strain is highly immunogenic and provide robust protective immunity against subsequent lethal L. monocytogenes challenge.
Publications
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Progress 01/01/12 to 12/31/12
Outputs OUTPUTS: No public presentation outputs to report. PARTICIPANTS: This project involved: 1) Joshua Obar, PI; experimental design and analysis; 2) Carly Grant, Undergraduate Research Assistant; conducted and analyzed the experiments; 3) Tyler Johnson, Master's Student; conducted experiments; 4) Julianne Zickovich, Research Associate; conducted and analyzed the experiments. TARGET AUDIENCES: Not relevant to this project. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.
Impacts These studies have shown us that the prsA2- htrA- Listeria monocytogenes mutant induces robust T cells responses which are highly protective against future Listeria monocytogenes infections. Specifically, the prsA2- htrA- Listeria monocytogenes mutant induces good maturation of antigen-presenting cells, which leads to robust induction of Listeria-specific memory CD4 and CD8 T cells. Thus, these data support the use of the prsA2- htrA- Listeria monocytogenes mutant as a vaccine vector. We will next examine the ability the prsA2- htrA- Listeria monocytogenes mutant to induce protective immunity during HKL + LM co-infection, a model of Circling Disease in ruminants.
Publications
- No publications reported this period
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