Progress 07/01/11 to 06/30/13
Outputs Target Audience: This project is directly related to the catfish health research community. Research outcomes should help gain more information on motile aeromonad septicemia in catfish and accelerate research on vaccine development for this disease. The targeted audiences also include fish health community, catfish producers, and microbiologists. Changes/Problems: Transposon end mapping in A. hydrophila mutants has not been as efficient as we were expecting, which slowed down the progress. What opportunities for training and professional development has the project provided? A MS student, Safak Kalindamar, has been trained as part of his MS research. How have the results been disseminated to communities of interest? Findings were presented in the American Society for Microbiology South Central Branch Meeting and departmental seminar. What do you plan to do during the next reporting period to accomplish the goals?
Nothing Reported
Impacts What was accomplished under these goals?
Random transposon mutations have been introduced into Aeromonas hydrophila genome. Efforts are focused on identification of transposon insertion sites. In addition, potential vaccine targets on the A. hydrophila genome have been identified by submitting its genome to vaccine target prediction and analysis system.
Publications
- Type:
Journal Articles
Status:
Accepted
Year Published:
2012
Citation:
Kalindamar S, Fuqua K, Lu J, M, Lawrence ML, Karsi A. 2012. Identification of the Edwardsiella ictaluri genes causing impaired growth. American Society for Microbiology South Central Branch Meeting, Starkville, MS, USA.
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Progress 01/01/12 to 12/31/12
Outputs OUTPUTS: Random transposon mutations have been introduced into Aeromonas hydrophila genome. Effects of random transposon insertions are being analyzed to identify possible virulence related A. hydrophila genes. In addition, the A. hydrophila genome sequence has been submitted to vaccine target prediction and analysis system to predict potential vaccine targets. PARTICIPANTS: Dr. Attila Karsi is the project director and a MS student has been working on this project. TARGET AUDIENCES: This project is directly related to the catfish health research community. Research outcomes should help gain more information on motile aeromonad septicemia in catfish and accelerate research on vaccine development for this disease. The targeted audiences also include fish health community, catfish producers, and microbiologists. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.
Impacts We produced a random transposon mutant library from an epidemic strain of A. hydrophila. Functional characterization of interrupted genes is being conducted. Moreover, surface-exposed antigens with potential immunogenic properties have been identified, some of which will be expressed in a live attenuated Edwardsiella ictaluri strain.
Publications
- No publications reported this period
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Progress 01/01/11 to 12/31/11
Outputs OUTPUTS: Because of the late release of funds, this project started recently. PARTICIPANTS: Dr. Attila Karsi is the project director and a MS student has been assigned to this project. TARGET AUDIENCES: This project is directly related to the catfish health research community. Research outcomes should help gain more information on motile aeromonad septicemia in catfish and accelerate research on vaccine development for this disease. The targeted audiences also include fish health community, catfish producers, and microbiologists. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.
Impacts We have completed several conjugations to transfer plasmid carrying the bacterial lux genes into Aeromonas hydrophila. Although we were able to obtain bioluminescent A. hydrophila colonies, it appears plasmid stability should be improved. Research undertaken to date revealed that transposon mutagenesis works in A. hydrophila. Thus, we are in process of producing random transposon mutant library. Functional characterization of interrupted genes should lead to a better understanding of A. hydrophila pathogenesis. Moreover, surface-exposed antigens are being identified using bioinformatics tools.
Publications
- No publications reported this period
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